A5021152668- Mitochondrial Function and Pathology
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- CRISPR and Genetic Engineering
- Gut microbiota and health
- Retinoids in leukemia and cellular processes
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Dietary Effects on Health
- RNA Research and Splicing
- Biological Stains and Phytochemicals
- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- Protein Kinase Regulation and GTPase Signaling
- Histone Deacetylase Inhibitors Research
- Natural product bioactivities and synthesis
- Metabolism and Genetic Disorders
- Fibroblast Growth Factor Research
- Microtubule and mitosis dynamics
- Synthesis and bioactivity of alkaloids
Chungnam National University
2011-2025
Korea Research Institute of Bioscience and Biotechnology
2020-2025
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2014-2017
National Institutes of Health
2014
Vanderbilt University
2013
Regional Cancer Center
2009-2012
Daejeon University
2012
Jeonbuk National University
2009
Seoul National University Bundang Hospital
2007
Targeted disruption of STAT3 function has proven to be a useful cancer therapeutic approach by inducing apoptotic cell death. Cucurbitacin is currently under development as small molecule inhibitor trigger death in many cancers. Here, we systematically studied the molecular mechanisms underlying cucurbitacin-induced death, particular involvement autophagy. Treatment with cucurbitacin resulted non-apoptotic caspase-independent manner. Notably, enhanced excessive conversion lipidated LC3...
The orphan nuclear receptor ESRRA (estrogen related alpha) is critical in mitochondrial biogenesis and macroautophagy/autophagy function; however, the roles of intestinal function remain uncharacterized. Herein we identified that acts as a key regulator homeostasis by amelioration colonic inflammation through activation autophagic flux control host gut microbiota. Esrra-deficient mice presented with increased susceptibility to dextran sodium sulfate (DSS)-induced colitis upregulation...
Abstract TNF receptor 1 can activate signaling pathways leading to the activation of NF-κB. A20, an NF-κB-inducible protein, negatively regulates these and acts as anti-inflammatory mediator. Therefore, A20 is viewed a potential therapeutic target for inflammatory disease. In this study, we examined effect on OVA-induced allergic airway inflammation model in mice. We used adenovirus containing cDNA (Ad-A20) that was delivered intratracheally before OVA challenge. Single administration Ad-A20...
Leucine zipper/EF hand-containing transmembrane-1 (LETM1) is a mitochondrial inner membrane protein that was first identified in Wolf-Hirschhorn syndrome, and deleted nearly all patients with the syndrome. LETM1 encodes for human homologue of yeast Mdm38p, which mitochondria-shaping unclear function. Here, we describe LETM1-mediated regulation ATP production biogenesis. We show overexpression can induce necrotic cell death HeLa cells, reduces biogenesis production. acts as an anchor...
Abstract Background The gut microbiota plays a pivotal role in maintaining intestinal homeostasis and mitigating inflammation. orphan nuclear receptor ESRRA (estrogen-related alpha) has been identified as critical regulator of mitochondrial function autophagy, yet its modulation health remains unclear. Methods We investigated using Esrra-deficient mice dextran sodium sulfate (DSS)-induced colitis model. Colitis severity is through survival rate body weight. Gut barrier was evaluated...
For in vitro DNA assembly, enzymes with exonuclease activities have been utilized to generate relatively long recessed ends on fragments, which can anneal other fragments if they complementary nucleotide sequences. The combined construct be directly delivered competent cells, where the gaps and nicks between are completely rectified. We introduce a versatile sequence- ligation-independent cloning (SLIC) method called ’DNA Assembly Phosphorothioate (PT) T5 Exonuclease’ (DAPE), generates...
3-Phosphoinositide-dependent protein kinase-1 (PDK1) appears to play a central regulatory role in many cell signalings between phosphoinositide-3 kinase and various intracellular serine/threonine kinases. In resting cells, PDK1 is known be constitutively active further activated by tyrosine phosphorylation (Tyr(9) Tyr(373/376)) following the treatment of with insulin or pervanadate. However, little about mechanisms for this additional activation PDK1. Here, we report that SH2 domain Src,...
Objective. Resveratrol is a naturally occurring polyphenol, which possesses chemotherapeutic potential through its ability to trigger apoptosis. The objective of this study was investigate the major determinant for apoptotic cell death induction by resveratrol in fibroblast-like synoviocytes (FLS) derived from patients with RA.
Brazilin is an active compound of Caesalpinia sappan L. (Leguminosae), which possesses pro-apoptotic and anti-inflammation potentials depending on the specific cell type. However, it largely unknown whether autophagy implicated in mechanism underlying its chemotherapeutic anti-inflammatory effects rheumatoid arthritis (RA). Here, we show that treatment RA fibroblast-like synoviocytes (FLS) with brazilin results enhanced level autophagic flux, evidenced by accumulation autophagosome increased...
We report a PCR-induced artifact in testing for homologous recombination zebrafish. attempted to replace the lnx2a gene with donor cassette, mediated by TALEN induced double stranded cut. The construct was flanked homology arms of about 1 kb at 5’ and 3’ ends. Injected embryos (G0) were raised outcrossed wild type fish. A fraction progeny appeared have undergone desired recombination, as tested PCR using primer pairs extending from genomic DNA outside region site within cassette. However,...
Activation of protein kinase C (PKC) by phorbol 12-myristate 13-acetate (PMA) triggers cellular signals that lead to the activation transcription factor NF-kappaB (nuclear kappaB) in various cell types. In addition short-time PMA treatment, here we report prolonged exposure human colonic cancer epithelial cells treated with can also a persistent inhibition activation. selectively causes degradation IkappaB kinases (IKKs) including IKK-gamma and IKK-beta, subsequent tumor necrosis (TNF)...
Abstract Background RET/PTC (rearranged in transformation/papillary thyroid carcinomas) gene rearrangements are the most frequent genetic alterations identified papillary carcinoma. Although it has been established that kinase plays a crucial role intracellular signaling pathways regulate cellular transformation, growth, and proliferation epithelial cells, upstream leads to activation of is largely unknown. Based on observation high levels PLD expression human cancer tissues, we investigated...
Death receptor (DR) ligation elicits two different modes of cell death (necroptosis and apoptosis) depending on the cellular context. By screening a plant extract library from cells undergoing necroptosis or apoptosis, we identified water Terminalia chebula (WETC) as novel potent dual inhibitor DR-mediated death. Investigation underlying mechanisms its anti-necroptotic anti-apoptotic action revealed that WETC constituents (e.g., gallic acid) protected against tumor necrosis factor-induced...
The RNA-binding protein Rbfox3 is a well-known splicing regulator that used as marker for post-mitotic neurons in various vertebrate species. Although recent studies indicate variable expression of non-neuronal tissues, including lung tissue, its cellular function cancer remains largely unknown. Here, we report the number RBFOX3-positive cells tumorous tissue lower than normal tissue. As transforming growth factor-β (TGF-β) signaling pathway important progression, investigated role RBFOX3...
Previous studies have demonstrated that rottlerin, a specific PKCδ inhibitor, potentiates death receptormediated apoptosis through cytochrome c-dependent or -independent pathway. However, its ability to regulate necrotic cell death, as well the underlying mechanism, remains unknown. We found in murine fibrosarcoma L929 cells, treatment with rottlerin protected cells against TNF-induced necrosis, whereas it sensitized induced by co-treatment Hsp90 inhibitor geldanamycin and TNF, manner...
The interleukin-6 (IL-6) family of cytokines is associated with retinal ganglion cell (RGC) survival and glial reactivity in glaucoma. purpose this study was to evaluate glaucoma-related changes glycoprotein-130 (gp130), the common signal transducer IL-6 cytokines, as they relate RGC health, expression cytokine members.For all experiments, we examined healthy retina (young C57), aged (aged predisposed glaucoma DBA/2) IOP-induced DBA/2). We determined gene gp130 members, using quantitative...
March8 is a member of family transmembrane E3 ubiquitin ligases that have been studied mostly for their role in the immune system. We find expressed zebrafish egg and early embryo, suggesting development. Both knock-down overexpression leads to abnormal The phenotype embryos Xenopus animal explants overexpressing implicates impairment cell adhesion as cause effect. In cultured cells, reduction surface levels E-cadherin, major cell-cell molecule. Experiments culture further show E-cadherin...