A5103135785- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Nuclear Structure and Function
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Systemic Sclerosis and Related Diseases
- Hepatitis C virus research
- Viral Infections and Immunology Research
- Acute Myeloid Leukemia Research
- Hepatitis B Virus Studies
Yale University
2016-2022
Newron Pharmaceuticals (Italy)
2011
Novartis (Italy)
2011
Nuclear export factor 1 (NXF1) exports mRNA to the cytoplasm after recruitment by specific adaptor proteins. How and why cells use numerous different adaptors is poorly understood. Here we critically evaluate members of SR protein family (SRSF1–7) for their potential act as NXF1 that couple pre-mRNA processing export. Consistent with this proposal, >1000 endogenous mRNAs required individual proteins nuclear in vivo. To address mechanism, transcriptome-wide RNA-binding profiles SRSF1–7...
SR proteins function in nuclear pre-mRNA processing, mRNA export, and translation. To investigate their cellular dynamics, we developed a quantitative assay, which detects differences nucleocytoplasmic shuttling among seven canonical protein family members. As expected, SRSF2 SRSF5 shuttle poorly HeLa cells but surprisingly display considerable pluripotent murine P19 cells. Combining individual-resolution cross-linking immunoprecipitation (iCLIP) mass spectrometry, show that elevated...
RNA binding proteins (RBPs) regulate the lives of all RNAs from transcription, processing, and function to decay. How RNA-protein interactions change over time space support these roles is poorly understood. Towards this end, we sought determine how two SR proteins-SRSF3 SRSF7, regulators pre-mRNA splicing, nuclear export translation-interact with in different cellular compartments. To do so, developed Fractionation iCLIP (Fr-iCLIP), which chromatin, nucleoplasmic cytoplasmic fractions are...
Musashi 2 (MSI2) is an RNA binding protein (RBP) that regulates asymmetric cell division and fate decisions in normal cancer stem cells. MSI2 appears to repress translation by 3' untranslated regions (3'UTRs) of mRNA, but the identity functional targets remains unknown. Here, we used individual nucleotide resolution cross-linking immunoprecipitation (iCLIP) identify direct partners integrated these data with polysome profiling obtain insights into function. iCLIP revealed specific thousands...
Hepatitis C Virus E1E2 heterodimers are components of the viral spike. Although there is a general agreement on necessity co-expression both E1 and E2 single coding unit for their productive folding assembly, in previous study using an vitro system we obtained strong indications that can achieve absence E2. Here, have studied pathway unescorted from stably expressing CHO cells, compared to observed presence protein. A DTT-resistant conformation achieved by situations, consistent with...
<h3>Background:</h3> There is a limited offer of fully-automated multiplexed devices for testing autoantibodies associated with connective tissue diseases (CTD). <h3>Objectives:</h3> To assess the performance characteristics novel MosaiQ<sup>®</sup> AiPlex CTD microarray immunoassay (AiPlex-CTD) when used MosaiQ system, simultaneous qualitative detection eleven commonly found in CTD. <h3>Methods:</h3> A comparator study was conducted at Hôpital Pitié-Salpêtrière (Paris, France), using...
Abstract Somatic mutations in splicing factors are of significant interest myeloid malignancies and other cancers. U2AF1, together with U2AF2, is essential for 3’ splice site recognition. U2AF1 result aberrant splicing, but the molecular mechanism full spectrum consequences on RNA biology have not been fully elucidated to date. We performed multi-omics profiling vivo binding, turnover S34F Q157R mutants. dissected specific binding signals U2AF2 showed that individually alter U2AF1-RNA...
ABSTRACT Musashi 2 (MSI2) is an RNA binding protein (RBP) that regulates asymmetric cell division and fate decisions in normal cancer stem cells. MSI2 appears to repress translation by 3’ untranslated regions (3’UTRs) of mRNA, but the identity functional targets remains unknown. Here we used iCLIP identify direct partners integrated these data with polysome profiling obtain insights into function. revealed specific thousands target mRNAs largely 3’UTRs, translational differences were...