A5037710598- Glutathione Transferases and Polymorphisms
- RNA and protein synthesis mechanisms
- Parasitic Infections and Diagnostics
- Vector-borne infectious diseases
- Phytase and its Applications
- Genomics, phytochemicals, and oxidative stress
- RNA Research and Splicing
- Amoebic Infections and Treatments
- Pediatric Hepatobiliary Diseases and Treatments
- Essential Oils and Antimicrobial Activity
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Curcumin's Biomedical Applications
- RNA modifications and cancer
- Cellular transport and secretion
- Mosquito-borne diseases and control
- Flowering Plant Growth and Cultivation
- Natural product bioactivities and synthesis
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- Cassava research and cyanide
- Viral Infections and Vectors
- Pharmacological Effects of Medicinal Plants
- Garlic and Onion Studies
Frederick National Laboratory for Cancer Research
2013-2025
Yale University
2016-2024
Siksha O Anusandhan University
2023-2024
Manipal University Jaipur
2021-2023
Galgotias University
2023
Yale Cancer Center
2022
National Agri-Food Biotechnology Institute
2019-2021
National Institutes of Health
2020
National Cancer Institute
2014-2020
Central University of South Bihar
2019
Abstract Human babesiosis is a potentially fatal tick-borne disease caused by intraerythrocytic Babesia parasites. The emergence of resistance to recommended therapies highlights the need for new and more effective treatments. Here we demonstrate that 8-aminoquinoline antimalarial drug tafenoquine inhibits growth different species in vitro, highly against microti duncani mice protects animals from lethal infection atovaquone-sensitive -resistant B. strains. We further show combination...
Withaferin A (WA), a C5,C6-epoxy steroidal lactone derived from medicinal plant (Withania somnifera), inhibits growth of human breast cancer cells in vitro and vivo prevents mammary development transgenic mouse model. However, the mechanisms underlying anticancer effect WA are not fully understood. Herein, we report that tubulin is novel target WA-mediated arrest cells. The G2 mitotic resulting exposure MCF-7, SUM159, SK-BR-3 was associated with marked decrease protein levels β-tubulin....
Loss of function mutations in the actin motor myosin Vb (Myo5b) lead to microvillus inclusion disease (MVID) and death newborns children. MVID results secretory diarrhea, brush border (BB) defects, villus atrophy, inclusions (MVIs) enterocytes. How loss Myo5b increased stool chloride (Cl − ) sodium (Na + is unknown. The present study used loss-of-function human intestine, polarized intestinal cell models crypt (T84) resembling (CaCo2BBe, C2BBe) enterocytes lacking conjunction with...
We report the antitumor effects of nitric oxide (NO) releasing derivatives PARP-1 inhibitor olaparib (1). Compound 5b was prepared by coupling carboxyl group 3b and free amino arylated diazeniumdiolated piperazine 4. Analogue 5a has same structure except that F is replaced H. 13 as a Me2N-N(O)═NO- added para ortho to nitro groups dinitrophenyl ring. The resulting prodrugs are activated glutathione in reaction accelerated S-transferase P1 (GSTP1), an enzyme frequently overexpressed cancers....
Musashi 2 (MSI2) is an RNA binding protein (RBP) that regulates asymmetric cell division and fate decisions in normal cancer stem cells. MSI2 appears to repress translation by 3' untranslated regions (3'UTRs) of mRNA, but the identity functional targets remains unknown. Here, we used individual nucleotide resolution cross-linking immunoprecipitation (iCLIP) identify direct partners integrated these data with polysome profiling obtain insights into function. iCLIP revealed specific thousands...
RAS is the most frequently mutated oncogene in cancer. proteins show high sequence similarities their G-domains but are significantly different C-terminal hypervariable regions (HVR). These interact with cell membrane via lipid anchors that result from posttranslational modifications (PTM) of cysteine residues. KRAS4b unique as it has only one undergoes PTM, C185. Small molecule covalent modification C185 would block any form prenylation and subsequently inhibit attachment to membrane,...
Dietary isothiocyanates abundant as glucosinolate precursors in many edible cruciferous vegetables are effective for prevention of cancer chemically-induced and transgenic rodent models. Some these agents, including phenethyl isothiocyanate (PEITC), have already advanced to clinical investigations. The primary route metabolism is its conjugation with glutathione (GSH), a reaction catalyzed by S-transferase (GST). pi class GST subunit type 1 (hGSTP1) much more than the alpha (hGSTA1)...
Coronary artery disease (CAD) has the highest mortality rate; therefore, its diagnosis is vital. Intravascular ultrasound (IVUS) a high-resolution imaging solution that can image coronary arteries, but software via wall segmentation and quantification been evolving. In this study, deep learning (DL) paradigm was explored along with bias.Using PRISMA model, 145 best UNet-based non-UNet-based methods for were selected analyzed their characteristics scientific clinical validation. This study...
Plant tissue culture is emerging as a pivotal biotechnological tool with profound implications for medical therapy, particularly in the realm of herbal medicine. Medicinal plants have long been cherished their natural healing properties. However, escalating demand, habitat destruction, and overharvesting threatened availability sustainability these valuable resources. addresses concerns by enabling mass propagation medicinal plants. In controlled environments, plant tissues can be multiplied...
Abstract Background The major clinical manifestations of Entamoeba histolytica infection include amebic colitis and liver abscess. However the majority infections remain asymptomatic. Earlier reports have shown that some E. isolates are more virulent than others, suggesting virulence may be linked to genotype. Here we looked at genomic distribution retrotransposable short interspersed nuclear elements EhSINE1 EhSINE2. Due their mobile nature, EhSINE copies occupy different locations among...
Staphylococcus aureus is a notorious pathogen which often causes nosocomial and community attained infections. These infections steadily increased after evolving the resistance due to indecorous practice of antibiotics now become serious health issue. Ouabain Na+/K+-ATPase inhibitor that leads increase heart contraction in patients with congestive failure.In present study, vitro antimicrobial effect ouabain together aminoglycosides was determined against clinical non-clinical S. strains....
Abstract Background Entamoeba histolytica and dispar are closely related protistan parasites but while E. can be invasive, is completely non pathogenic. Transposable elements constitute a significant portion of the genome in these species; there being three families LINEs SINEs. These profoundly influence expression neighboring genes. Thus their genomic location have important phenotypic consequences. A genome-wide comparison genomes has not been carried out. It also known whether...
Babesiosis is a tick-borne disease caused by pathogens belonging to the genus Babesia. In humans, presents as malaria-like illness and can be fatal in immunocompromised elderly people. past few years, human babesiosis has been rising concern worldwide. The transmitted through tick bite, blood transfusion, transplacentally rare cases, with several species of Babesia causing infection. microti, duncani, divergens are particular interest because their important health impact amenability...
Small nucleolar RNAs are a highly conserved group of small found in eukaryotic cells. Genes encoding these diversely located throughout the genome. They functionally conserved, performing post transcriptional modification (methylation and pseudouridylation) rRNA other nuclear RNAs. belong to two major categories: C/D box H/ACA containing snoRNAs. U3 snoRNA is an exceptional member snoRNAs involved early processing pre-rRNA. An antisense sequence present each which guides or target RNA....
Abstract Background Inositol pyrophosphates (PP-InsPs) are high-energy derivatives of inositol, involved in different signalling and regulatory responses eukaryotic cells. Distinct PP-InsPs species characterized by the presence phosphate at a variable number 6-carbon inositol ring backbone, two distinct classes kinases responsible for their synthesis have been identified Arabidopsis, namely ITPKinase (inositol 1,3,4 trisphosphate 5/6 kinase) PP-IP5Kinase (diphosphoinositol pentakisphosphate...
The significant rise in the number of tick-borne diseases represents a major threat to public health worldwide. One such emerging disease is human babesiosis, which caused by several protozoan parasites Babesia genus B. microti responsible for most clinical cases reported date. Recent studies have shown that during its intraerythrocytic life cycle, exports antigens into mammalian host using novel vesicular-mediated secretion mechanism. these secreted proteins immunodominant antigen BmGPI12,...
Cell growth and division are coordinated, ensuring homeostasis under any given condition, with occurring as cell mass doubles. The signals controlling circuit(s) between not well understood; however, it is known in Escherichia coli that the essential GTPase Era, which rate regulated, coordinates two functions may be a checkpoint regulator of both. We have isolated mutant Era separates its effect on division. When overproduced, protein Era647 dominant to wild-type blocks division, causing...
Echinocandin B is a natural product that possesses potent antifungal property against wide array of fungi. This agent produced by Emericella rugulosa. The biosynthetic genes echinocandin are physically organized in two gene clusters (ecd and hty). ecd cluster comprises 12 (ecdA–L). On the other hand, hty contains six (htyA–F). These regulate expression play an essential role chromatin modifications. present study elucidates epigenetic regulatory network production using Histone...
This comprehensive overview delves into the intricate techniques involved in seed production for flowering annuals. From selecting suitable varieties to understanding pollination dynamics, harvesting, and storage, this review provides valuable insights complex process of production. Drawing upon latest research practical considerations, it highlights importance proper management practices maintaining genetic purity viability. By exploring various methodologies abstract aims provide a...
Twelve treatment combinations of the date planting and gibberellic acid corm were replicated three times in Randomized Block Design (RBD) factorial concept investigation conducted Gladiolus (Gladiolus grandiflorus L.) cv. Arka Amar during 2022–2023 rabi season at SOADU, Bhubaneswar (Odisha). The findings showed that on September 15, dipping corms with 150 ppm GA3 concentration resulted maximum number sprouting (2.56), plant height (62.42 cm), floret per spike (20.72), length (11.56 rachis...