A5039755803- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Eosinophilic Esophagitis
- Neuroinflammation and Neurodegeneration Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Immune Cell Function and Interaction
- Veterinary medicine and infectious diseases
- Immunotherapy and Immune Responses
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- IL-33, ST2, and ILC Pathways
- Chemokine receptors and signaling
- Reproductive System and Pregnancy
- Dermatology and Skin Diseases
- Digestive system and related health
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Reproductive tract infections research
- Tryptophan and brain disorders
- Genetics, Bioinformatics, and Biomedical Research
- HIV, Drug Use, Sexual Risk
- Immune cells in cancer
Dana-Farber Cancer Institute
2007-2015
Harvard University
2007-2014
The Ohio State University
2000-2013
Interface (United States)
2013
Boston University
2010
National Cancer Institute
2004
Case Western Reserve University
2004
Cornell University
2004
Tulane University
2003-2004
University of Geneva
2004
Elevated plasma lipopolysaccharide (LPS), an indicator of microbial translocation from the gut, is a likely cause systemic immune activation in chronic HIV infection. LPS induces monocyte and trafficking into brain, which are key mechanisms pathogenesis HIV-associated dementia (HAD). To determine whether high levels associated with increased HAD, we obtained peripheral blood samples AIDS patients examined by Limulus amebocyte lysate (LAL) assay, monocytes FACS, soluble markers ELISA....
Topical agents, such as microbicides, that can protect against human immunodeficiency virus (HIV) transmission are urgently needed. Using a chimeric simian/human (SHIV SF162), which is tropic for the chemokine receptor CCR5, we report topical application of high doses PSC-RANTES, an amino terminus-modified analog RANTES, provided potent protection vaginal challenge in rhesus macaques. These experimental findings have potentially important implications understanding HIV and design strategies...
Gastritis due to Helicobacter pylori in mice and humans is considered a Th1-mediated disease, but the specific cell subsets cytokines involved are still not well understood. The goal of this study was investigate immunopathogenesis H. pylori-induced gastritis delayed-type hypersensitivity (DTH) mice. C57BL/6-Prkdc(scid) were infected with reconstituted CD4+, CD4-depleted, CD4+CD45RB(high), or CD4+CD45RB(low) splenocytes from wild-type C57BL/6 C57BL/6(IFN-gamma-/-) C57BL/6(IL-10-/-) Four...
Binding to the CD4 receptor induces conformational changes in human immunodeficiency virus (HIV-1) gp120 exterior envelope glycoprotein. These allow bind coreceptor, either CCR5 or CXCR4, and prime gp41 transmembrane glycoprotein mediate virus-cell membrane fusion entry. Soluble forms of (sCD4) small-molecule mimics (here exemplified by JRC-II-191) also induce these HIV-1 glycoproteins, but typically inhibit entry into CD4-expressing cells. To investigate mechanism inhibition, we monitored...
Human immunodeficiency virus type 1 (HIV-1) fuses with cells after sequential interactions between its envelope glycoproteins, CD4 and a coreceptor, usually CC chemokine receptor 5 (CCR5) or CXC 4 (CXCR4). CMPD 167 is CCR5-specific small molecule potent antiviral activity in vitro. We show that caused rapid substantial (4–200-fold) decrease plasma viremia six rhesus macaques chronically infected simian (SIV) strains SIVmac251 SIVB670, but not an animal the X4 simian–human (SHIV), SHIV-89.6P....
Background: Accumulating data suggest that the state of vaginal epithelium affects a woman's risk HIV transmission and several human non-human primate studies have shown rate or SIV is decreased when estrogen dominant. Systemic can protect against transmission. Objective: To determine safety efficacy topical in preventing Design: The model was used to assess estriol cream ovariectomized macaques. Methods: Twelve macaques were treated intravaginally with eight placebo twice week. systemic...
Previous studies have shown that vaccination and boosting of rhesus macaques with attenuated vesicular stomatitis virus (VSV) vectors encoding Env Gag proteins simian immunodeficiency virus-human (SHIV) hybrid viruses protect from AIDS after challenge the highly pathogenic SHIV 89.6P (23). In present study, we compared effectiveness a single prime-boost protocol consisting VSV expressing Env, Gag, Pol to vector prime followed boost modified vaccinia Ankara (MVA) same proteins. After 89.6P,...
ABSTRACT The Helicobacter pylori chromosomal region known as the cytotoxin-gene associated pathogenicity island ( cag PAI) is with severe disease and encodes proteins that are believed to induce interleukin (IL-8) secretion by cultured epithelial cells. objective of this study was evaluate relationship between PAI, induction IL-8, neutrophilic gastric inflammation. Germ-free neonatal piglets conventional C57BL/6 mice were given wild-type or deficient mutant derivatives H. strain 26695 SS1....
ABSTRACT Vaccination suppresses Helicobacter pylori colonization but does not cure infection. Furthermore, postvaccination gastritis, likely induced by enhanced host response to residual colonization, may exacerbate disease. The goal of this study was determine if adoptive transfer C57BL/6 splenocytes scid/scid (severe combined immunodeficient [SCID]) mice cures infection without exacerbating gastritis. H. -infected and uninfected SCID recipients normal were killed at intervals between 5 51...
Human immunodeficiency virus (HIV-1) variants in brain primarily use CCR5 for entry into macrophages and microglia, but dual-tropic (R5X4) HIV-1 has been detected cerebral spinal fluid (CSF) of some patients with HIV-associated dementia (HAD). Here, we sequenced the gp120 coding region nine full-length env genes cloned directly from autopsy spleen tissue an AIDS patient severe HAD. We then compiled a dataset 30 unique clade B R5X4 Env V3 sequences this subject 16 additional (n = 4 26...
Abstract Background The population of HIV replicating within a host consists independently evolving and interacting sub-populations that can be genetically distinct anatomical compartments. the brain causes neurocognitive disorders in up to 20-30% infected individuals is viral sanctuary site for development drug resistance. primary determinant neurotropism macrophage tropism, which primarily determined by envelope ( env ) gene. However, studies genetic aspects are hindered because existing...
Macrophages play an important role in HIV/SIV pathogenesis by serving as a reservoir for viral persistence brain and other tissues. Infected macrophages have been detected early after infection, but macrophage-tropic viruses are rarely isolated until late-stage infection. Little is known about variants that establish persistent infection brain. Here, we characterize unique SIV envelope glycoprotein (Env) variant from two weeks post-infection blood of SIVmac251-infected macaque closely...
Approximately 20% of all HIV-1 mother-to-child transmission (MTCT) occurs in utero (IU). In a chronic HIV infection, exists as complex swarm genetic variants, and following IU MTCT, viral genomic diversity is restricted through mechanism that remains to be described. The 5' U3R region the long terminal repeat (LTR) contains multiple transcription factor (TF) binding sites regulates transcription. this study, we tested hypothesis sequence polymorphisms LTR are associated with MTCT. To end,...