A5101566722- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Soybean genetics and cultivation
- Plant and Fungal Interactions Research
- Plant responses to water stress
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Seed Germination and Physiology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Machine Learning in Bioinformatics
- Genetic and Environmental Crop Studies
- Enterobacteriaceae and Cronobacter Research
- Insect symbiosis and bacterial influences
- Mycobacterium research and diagnosis
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Genetically Modified Organisms Research
- Genomics and Phylogenetic Studies
- Influenza Virus Research Studies
Institute of Crop Sciences
2015-2025
Chinese Academy of Agricultural Sciences
2015-2025
Genome editing is a valuable technique for gene function analysis and crop improvement. Over the past two years, CRISPR-Cas9 system has emerged as powerful tool precisely targeted editing. In this study, we predicted 11 U6 genes in soybean (Glycine max L.). We then constructed vectors (pCas9-GmU6-sgRNA pCas9-AtU6-sgRNA) using U6-10 Arabidopsis U6-26 promoters, respectively, to produce synthetic guide RNAs (sgRNAs) mutagenesis. Three genes, Glyma06g14180, Glyma08g02290 Glyma12g37050, were...
We found GmNAC06 plays an important role in salt stress responses through the phenotypic, physiological and molecular analyses of OE, VC, Mutant composite soybean. Salinization affects 20% all cultivated land worldwide because high salinity irrigation water excessive use water, this amount is increasing daily. NAC (NAM, ATAF, CUC) have been to be involved stress. In study, a soybean gene, (Glyma06g21020.1), was cloned functionally characterized. The results expression analysis suggested that...
Small auxin-upregulated RNAs (SAURs) are genes regulated by auxin and environmental factors. In this study, we identified a SAUR gene in wheat, TaSAUR75. Under salt stress, TaSAUR75 is downregulated wheat roots. Subcellular localization revealed that was localized both the cytoplasm nucleus. Overexpression of increased drought tolerance Arabidopsis. Transgenic lines showed higher root length survival rate expression some stress-responsive than control plants under stress. Less H2O2...
Environmental stimuli can activate a series of physiological and biochemical responses in plants accompanied by extensive transcriptional reprogramming. Long non-coding RNAs (lncRNAs), as versatile regulators, control gene expression multiple ways participate the adaptation to biotic abiotic stresses.In this study, soybean seedlings were continuously cultured for 15 days with high salinity solutions started from seed germination. Strand-specific whole transcriptome sequencing stringent...
Emerging evidence indicates that long non-coding RNAs (lncRNAs) play important roles in diverse biological processes. However, the functions of most plant lncRNAs are still unknown. We previously discovered a soybean abiotic-stress-related lncRNA, lncRNA77580, and cloned entire full-length sequence. Here, order to fully identify function lncRNA77580 stress response, we created transgenic lines overexpressing lncRNA77580. Compared with wild type, overexpression enhances drought tolerance...
RNA silencing such as quelling and meiotic by unpaired DNA (MSUD) several other classes of special small RNAs have been discovered in filamentous fungi recently. More than four different mechanisms microRNA-like (milRNAs) production illustrated the model fungus Neurospora crassa including a dicer-independent pathway. To date, very little work focusing on has reported no universal or particular characteristic milRNAs were defined clearly. In this study, degradome libraries constructed...
Rice bean (Vigna umbellata) is an underexploited domesticated legume crop consumed for dietary protein in Asia, yet little known about the genetic diversity of this species. Here, we present a high-quality reference genome rice landrace (FF25) built using PacBio long-read data and Hi-C chromatin interaction map, assess phylogenetic position speciation time within Vigna genus. We sequence 440 landraces (two core collections), GWAS based on growth sites at three widely divergent latitudes...
MicroRNAs (miRNAs) play important roles in post-transcriptional gene silencing by directing target mRNA cleavage or translational inhibition. Currently, hundreds of miRNAs have been identified plants, but no report has published wild soybean (Glycine soja Sieb). We constructed a small-RNA library consisting 2 880 sequences with high quality, which 1 347 were 19-24 nt length. By utilizing the miRNA, Rfam and domesticated expressed sequence tag database, we analyzed predicted secondary...
The transcription factor dehydration-responsive element binding protein (DREB) is able to improve tolerance abiotic stress in plants by regulating the expression of downstream genes involved environmental resistance. objectives this study were evaluate salt GmDREB1 transgenic wheat (Triticum aestivum L.) and its physiological responses stress. Compared with wild type, lines overexpressing showed longer coleoptiles radicles a greater radicle number at germination stage, as well root length,...
Salinity is a major abiotic stress that affects plant growth and development. Plant roots are the sites of salt uptake. Here, an isobaric tag for relative absolute quantitation based proteomic technique was employed to identify differentially expressed proteins (DEPs) from seedling salt-tolerant genotype Han 12 salt-sensitive Jimai 19 in response treatment. A total 121 NaCl-responsive DEPs were observed 19. The main ubiquitination-related proteins, transcription factors, pathogen-related...
Klebsiella pneumoniae is a significant pathogen capable of causing infections in the respiratory, urinary, and bloodstream. In this study, bioinformatics-based pan-genome analysis identified specific conserved LptD MerR protein gene sequences K. pneumoniae. Based on these sequences, primers probes were designed, reaction system conditions optimized to establish TaqMan probe-based duplex real-time quantitative PCR method for detecting The was tested genomic DNA from 14 common pathogens...
The NAC (NAM, ATAF1/2 and CUC2) transcription factor family plays a key role in plant development responses to abiotic stress. GmNAC15 (Glyma15g40510.1), member of the soybean, was functionally characterized, especially with regard its salt tolerance. In present study, qRT-PCR (quantitative reverse PCR) analysis indicated that induced by salt, drought, low temperature stress, ABA treatment roots leaves. overexpression soybean (Glycine max) hairy enhanced Transgenic improved survival wild...
Plant microRNAs (miRNAs) regulate gene expression mainly by guiding cleavage of target mRNAs. In this study, a degradome library constructed from different soybean (Glycine max (L.) Merr.) tissues was deep-sequenced. 428 potential targets small interfering RNAs and 25 novel miRNA families were identified. A total 211 targets, including 174 conserved 37 soybean-specific Among them, 121 first discovered in soybean. The signature distribution primary miRNAs (pri-miRNAs) showed that most...
SMALL AUXIN-UP RNAs (SAURs) regulated by abiotic stress play multiple functions in plants. However, the of SAURs are largely unknown. In this study, we cloned a novel SAUR gene, TaSAUR78, from wheat, and found that TaSAUR78 interacted with TaVDAC1 (voltage-dependent anion channel). Salt decreased expression increased TaVDAC1. Overexpression enhanced tolerance to salt, drought, freezing stresses transgenic Arabidopsis reduced accumulation reactive oxygen species (ROS) under salt stress....
The engineering of plants with enhanced tolerance to abiotic stresses typically involves complex multigene networks and may therefore have a greater potential introduce unintended effects than the genetic modification for simple monogenic traits. For this reason, it is essential study in transgenic engineered stress tolerance. We selected drought- salt-tolerant wheat overexpressing transcription factor, GmDREB1, investigate pleiotropic using RNA-seq analysis. compared transcriptome...
Abstract Background Natural antisense transcripts (NATs) are a class of RNAs that contain sequence complementary to other transcripts. NATs occur widely in eukaryotes and play critical roles post-transcriptional regulation. Soybean NAT sequences predicted the PlantNATsDB, but detailed analyses these remain be performed. Results A total 26,216 NATs, including 994 cis -NATs 25,222 trans -NATs, were soybean. Each sense transcript had 1–177 We identified 21 trans-NATs using RT-PCR amplification....
Long non-coding RNAs (lncRNAs) affect gene expressions via a wide range of mechanisms and are considered important regulators numerous essential biological processes, including abiotic stress responses. However, the functions most lncRNAs yet to be determined. Moreover, date, no effective methods have been developed study function plant lncRNAs. We previously discovered salt stress-related lncRNA, lncRNA77580 in soybean (Glycine max L.). In this study, we cloned full-length found that it...
MicroRNAs (miRNAs) are small regulators of gene expression that act on many different molecular and biochemical processes in eukaryotes. To date, miRNAs have not been considered the current evaluation system for GM crops. In this study, RNAs from dry seeds a wheat line overexpressing GmDREB1 non-GM cultivars were investigated using deep sequencing technology bioinformatic approaches. As result, 23 differentially expressed identified confirmed between acceptor. Notably, more tae-miRNAs...