A5101890533- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Soil Carbon and Nitrogen Dynamics
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Crop Yield and Soil Fertility
- Plant responses to water stress
- Plant Micronutrient Interactions and Effects
- Plant Gene Expression Analysis
- Rice Cultivation and Yield Improvement
- Agriculture, Soil, Plant Science
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Resistance
- Plant and animal studies
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Garlic and Onion Studies
- Plant Growth Enhancement Techniques
- Plant Virus Research Studies
- Plant Surface Properties and Treatments
- Postharvest Quality and Shelf Life Management
- Ecology and Vegetation Dynamics Studies
Shandong Agricultural University
2016-2025
Institute of Vegetables and Flowers
2019-2025
Chinese Academy of Agricultural Sciences
2017-2025
Guizhou University
2014-2025
Nankai University
2023-2024
Guangxi University
2021-2024
Guangxi Zhuang Autonomous Region Health and Family Planning
2024
Ministry of Agriculture and Rural Affairs
2021-2024
Northeast Agricultural University
2011-2024
Leshan Normal University
2024
We show that NITRATE REGULATORY GENE2 (NRG2), which we identified using forward genetics, mediates nitrate signaling in Arabidopsis thaliana. A mutation NRG2 disrupted the induction of nitrate-responsive genes after treatment by an ammonium-independent mechanism. The content roots was lower mutants than wild type, may have resulted from reduced expression NRT1.1 (also called NPF6.3, encoding a transporter/receptor) and upregulation NRT1.8 NPF7.2, xylem transporter). Genetic molecular data...
Abstract PHO1 has been recently identified as a protein involved in the loading of inorganic phosphate into xylem roots Arabidopsis. The genome Arabidopsis contains 11 members gene family. cDNAs all homologs have cloned and sequenced. All proteins same topology harbor SPX tripartite domain N-terminal hydrophilic portion an EXS C-terminal hydrophobic portion. domains yeast (Saccharomyces cerevisiae) either transport or sensing sorting to endomembranes. additional unknown function containing...
Abstract Nitrate regulatory mutants (nrg) of Arabidopsis (Arabidopsis thaliana) were sought using a genetic screen that employed nitrate-inducible promoter fused to the yellow fluorescent protein marker gene YFP. A mutation was identified impaired nitrate induction, and it localized NLP7, demonstrating validity this screen. second, independent (nrg1) mapped region containing NRT1.1 (CHL1) transporter on chromosome 1. Sequence analysis in mutant revealed nonsense truncated at amino acid 301....
The PHO1 family comprises 11 members in Arabidopsis thaliana. In order to decipher the role of these genes inorganic phosphate (Pi) transport and homeostasis, complementation pho1 mutant, deficient loading Pi root xylem, was determined by expression homologous under control promoter. Only homologue PHO1;H1 could complement pho1. promoter active vascular cylinder roots shoots. Expression very low Pi-sufficient plants, but strongly induced Pi-deficient conditions. T-DNA knock-out mutants...
Adverse environmental conditions, including high temperature, often affect the growth and production of crops worldwide. F-box protein, a core component Skp1-Cullin-F-box (SCF) E3 ligase complex, plays an important role in abiotic stress responses. A previously cloned gene from wheat, TaFBA1, encodes homologous protein. Yeast two-Hybrid (Y2H) assay showed that TaFBA1 interacted with other SCF proteins. We found expression was induced by heat (45°C). Overexpression enhanced tolerance...
In plants, the chilling response involves decreased photosynthetic capacity and increased starch accumulation in chloroplasts. However, mechanisms that modulate these processes remain unclear. We found SlWHY1 gene is significantly induced by stress (4°C) tomato. Three overexpression (OE) lines grew better than wild type (WT) under stress; OE plants retained intact grana lamellae showed enhanced hydrolysis of starch. By contrast, RNAi inhibited were more affected corresponding WT cultivar....
RNA silencing such as quelling and meiotic by unpaired DNA (MSUD) several other classes of special small RNAs have been discovered in filamentous fungi recently. More than four different mechanisms microRNA-like (milRNAs) production illustrated the model fungus Neurospora crassa including a dicer-independent pathway. To date, very little work focusing on has reported no universal or particular characteristic milRNAs were defined clearly. In this study, degradome libraries constructed...
High temperature has become a major abiotic stress that limits crop productivity. Heat shock transcription factors (HSFs) and heat proteins (HSPs) play important roles in enhancing thermotolerance of plants. SUMOylation is an post-translational modification regulating cellular functions eukaryotes. SIZ1, well-characterized SUMO E3 ligase, mediates the process SUMOylation. In this study, conjugations were clearly induced by high temperature. Overexpression SIZ1 ligase (SlSIZ1) tomato could...
Long noncoding RNAs (lncRNAs) are crucial regulators in many plant biological processes. However, it remains unknown whether lncRNAs can respond to nitrate or function regulation. We detected 695 lncRNAs, 480 known and 215 novel, Arabidopsis seedling roots; six showed altered expression response treatment, among which T5120 the highest induction. Overexpression of promoted nitrate, enhanced assimilation improved biomass root development. Biochemical molecular analyses revealed that NLP7, a...
Agricultural soil is the largest source of nitrous oxide (N2O), a greenhouse gas. Soybean an important leguminous crop worldwide. hosts symbiotic nitrogen-fixing bacteria (rhizobia) in root nodules. In soybean ecosystems, N2O emissions often increase during decomposition Our previous study showed that reductase can be used to mitigate emission from fields nodule by inoculation with nosZ++ strains [mutants increased (N2OR) activity] Bradyrhizobium diazoefficiens. Here, we show reduced at...
The phytohormone abscisic acid (ABA) regulates plant growth and development, as well responses to various stresses, such salt drought. wheat TaFBA1 gene, which encodes an F-box protein, was previously identified in our laboratory by homologous cloning. We found that expression induced ABA drought stress. In this study, wild-type (WT), over-expressing (OEs), gene mutants, recovery (Rs) Arabidopsis plants were used. the germination rate, cotyledon greening root length, photosynthetic...
Nitrate is a key nutrient that affects maize growth and yield, much has yet to be learned about nitrate regulatory genes mechanisms in maize. Here, we identified nine ZmNLP analyzed the functions of two members signaling. qPCR results revealed broad pattern expression for different stages organs with highest levels transcript ZmNLP6 ZmNLP8. When ZmNLP8 were overexpressed Arabidopsis gene mutant nlp7-4, assimilation induction nitrate-responsive transgenic plants recovered WT levels,...
Abstract Nitrate is not only an important nutrient but also a signaling molecule for plants. A few of key molecular components involved in primary nitrate responses have been identified mainly by forward and reverse genetics as well systems biology, however, many underlining mechanisms regulation remain unclear. In this study, we show that the expression NRT1.1 , which encodes sensor transporter (also known CHL1 NPF6.3 ), modulated NIN-like protein 7 (NLP7). Genetic analyses indicate NLP7...
Summary Plants have evolved sophisticated mechanisms to adapt fluctuating environmental nitrogen availability. However, more underlying genes regulating the response nitrate yet be characterized. We report here identification of a regulatory mutant whose mutation mapped Cleavage and Polyadenylation Specificity Factor 30 gene ( CPSF 30‐L ). In mutant, induction nitrate‐responsive was inhibited independent ammonium conditions restored by expression wild‐type 65 kDa encoded . Molecular genetic...
Abstract Nitrate is both an important nutrient and a critical signaling molecule that regulates plant metabolism, growth, development. Although several components of the nitrate pathway have been identified, molecular mechanism remains unclear. Here, we showed growth-related transcription factors HOMOLOG OF BRASSINOSTEROID ENHANCED EXPRESSION2 INTERACTING WITH IBH1 (HBI1) its three closest homologs (HBIs) positively regulate in Arabidopsis thaliana. HBI1 rapidly induced by through NLP6 NLP7,...