A5100657087- Wheat and Barley Genetics and Pathology
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Microfluidic and Capillary Electrophoresis Applications
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- RNA modifications and cancer
- Nitrogen and Sulfur Effects on Brassica
- MicroRNA in disease regulation
- Analytical Chemistry and Chromatography
- Glutathione Transferases and Polymorphisms
- Hydrogels: synthesis, properties, applications
- Genetics and Plant Breeding
- Biochemical and Structural Characterization
- Plant Parasitism and Resistance
- Venomous Animal Envenomation and Studies
Ministry of Education of the People's Republic of China
2018-2024
Northwest University
2018-2024
Shandong Academy of Agricultural Sciences
2014-2023
National Engineering Research Center for Wheat
2014-2023
Fujian Provincial Hospital
2023
Fujian Medical University
2023
Henan Institute of Science and Technology
2022
China-Japan Friendship Hospital
2019-2022
Ministry of Agriculture and Rural Affairs
2021
Nanjing Agricultural University
2021
Recently, the CRISPR/Cas9 system has been widely used to precisely edit plant genomes. Due difficulty in Agrobacterium-mediated genetic transformation of wheat, reported applications were all based on biolistic transformation. In present study, we efficiently applied targeted mutagenesis common wheat (Triticum aestivum L.) protoplasts and transgenic T0 plants using delivered via Agrobacterium tumefaciens. Seven target sites three genes (Pinb, waxy DA1) selected construct individual...
Common wheat supplies vast amounts of dietary carbohydrate and protein for over 60% world population. With continued economic development, demand high-yielding varieties with premium grain quality is increasing. Grain a multigenic trait that simultaneously affected by many factors, it more complicated in hexaploid wheat. CRISPR/Cas9-based genome editing provides great opportunities to create allelic variations much faster precise manner (Li et al., 2020a, 2020b; Sanchez-Leon 2018; Zhang...
Abstract Nitrate is both an important nutrient and a critical signaling molecule that regulates plant metabolism, growth, development. Although several components of the nitrate pathway have been identified, molecular mechanism remains unclear. Here, we showed growth-related transcription factors HOMOLOG OF BRASSINOSTEROID ENHANCED EXPRESSION2 INTERACTING WITH IBH1 (HBI1) its three closest homologs (HBIs) positively regulate in Arabidopsis thaliana. HBI1 rapidly induced by through NLP6 NLP7,...
Summary Phased, small interfering RNAs (phasiRNAs) are important for plant anther development, especially male sterility. PhasiRNA biogenesis is dependent on genes like RNA polymerase 6 (RDR6), DICER‐LIKE 4 (DCL4), or DCL5 to produce 21‐ 24 nucleotide (nt) double‐strand RNAs. Here, we generated mutants of DCL4 , and RDR6 using CRISPR/Cas9 system studied their effects reproductive development phasiRNA production in wheat. We found that mutation caused sever consequence throughout starting...
Common wheat has a large genome with three subgenomes (A, B and D), making it challenging to create mutations at multiple genomic sites simultaneously. The CRISPR/Cas9 system offers game-changing tool for editing crop genomes (Chen et al., 2019). Three main strategies have been developed produce single-guide RNAs (sgRNAs), including the conventional multiplex tandem repeats of separate U3 or U6 promoters (TRSP), tRNA-processing (Xie 2015) ribozyme-processing (Gao Zhao, 2014). Although...
The CRISPR/Cas9 system has been successfully used in hexaploid wheat. Although it reported that the induced mutations can be passed to next generation, gene editing and transmission patterns later generations still need studied. In this study, we demonstrated could achieve efficient mutagenesis five wheat genes via Agrobacterium-mediated transformation of an sgRNA targeting D genome, both A B homologues three tri-genome guides all homologues. High mutation rates putative homozygous or...
Abstract Cervical cancer (CC) is ranked as the fourth most common that occurs in women universally, which normally causes pain lower belly. Plenty of studies have stated expression long non-coding RNAs (lncRNAs) linked to cellular development many kinds cancers. DSCAM-AS1 has been reported act an oncogene other types and aim our study was uncover function regulatory mechanism CC. In this research, findings presented up-regulated CC cells. led by enhancing cell proliferation, migration...
This study aimed to validate the function of CKX gene on grain numbers in wheat.we constructed and transformed a RNA interference expression vector TaCKX2.4 bread wheat line NB1. Southern blotting analysis was used select transgenic plants with single copy. The estimated by Quantitative real-time PCR (qRT-PCR) analysis. Finally, relation between validated.Totally, 20 positive independent events were obtained. Homozygous lines from 5 copy each selected evaluate per spike T3 generation....
The immobilization of enzyme is one the key issues both in field enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) alginate carrier. presence calcium cation, enzyme-alginate suspension could be cross-linked form beads with porous structure at room temperature, CALB was dispersed beads. Activity composite verified by hydrolysis reaction p-nitrophenol butyrate aqueous phase. effects parameters such as pH,...
Copy number variation (CNV) is a kind of chromosomal structural reorganization that has been detected, in this decade, mainly by high-throughput biological technology. Researchers have found CNVs are ubiquitous many species and accumulating evidence indicates closely related with complex diseases. The investigation alterations begun to reveal some important clues the pathologic causes diseases disease process. However, published studies focused on single and, so far, experimental results not...
PhasiRNAs (phased secondary siRNAs) play important regulatory roles in the development processes and biotic or abiotic stresses plants. Some of phasiRNAs involve reproductive grasses, which include two categories, 21-nt (nucleotide) 24-nt phasiRNAs. They are triggered by miR2118 miR2275 respectively, premeiotic meiotic anthers rice, maize other grass species. Wheat (Triticum aestivum) with three closely related subgenomes (subA, subB subD), is a model allopolyploid Knowledge about role...
Base editing using CRISPR technologies is an invaluable tool for crop breeding. One of the major base editors, adenine editor (ABE), has been successfully used in both model plants and many crops. However, owing to limited efficiency, ABE difficult apply polyploid crops such as allohexaploid bread wheat that often require simultaneous mutation multiple alleles fast We have designed a high-efficiency (WhieABE), newly developed high-activity adenosine deaminase TadA8e. In vivo vitro analysis...
Background: The role of thioredoxin-interacting protein (TXNIP) in lipopolysaccharide-induced liver injury mice has been reported, but the underlying mechanisms are poorly understood. Methods: We overexpressed deubiquitinase cells overexpressing TXNIP and then detected level to screen out regulating TXNIP; interaction between was verified by coimmunoprecipitation. After knockdown a overexpression Huh7 HepG2 cells, lipopolysaccharide used establish cellular inflammatory model explore...
ABSTRACT The immobilization of enzymes is one the key issues in both field enzymatic research and industrialization. In this article, we report a facile method for immobilizing Candida antarctica lipase B an alginate carrier. presence calcium cations, enzyme–alginate suspension was crosslinked to form beads with porous structure at room temperature, were well dispersed beads. chiral resolution α‐phenyl ethanol organic phase tested by effects reaction parameters, such as enzyme concentration,...
The NAM-B1 gene is a member of the NAC (NAM, ATAF, and CUC) transcription factor family plays an important role in regulating wheat grain protein content (GPC). ancestral allele has been discovered many tetraploid wild emmer (Triticum turgidum ssp. dicoccoides) accessions few domesticated (T. dicoccum), however, it rarely found hexaploid bread aestivum L.). There are no systematic reports on distribution alleles Chinese cultivars. In this study, 218 cultivars were investigated. collected...
Abstract Background Brain amyloid deposition is one of the main pathological characteristics Alzheimer’s disease (AD). Soluble oligomers formed during process that causes β-amyloid (Aβ) to aggregate into plaques are considered have major neurotoxicity. Currently, drug development for treatment has encountered serious difficulties. Our newly proposed solution accelerate aggregation Aβ reduce amount cytotoxic in brain tissue. This strategy differs from existing reducing total content and...
ThIPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought a major limiting factor wheat (Triticum aestivum L.) production. The present study investigated whether the application of could increase drought resistance transgenic wheat. codon-optimized was transferred into common through Agrobacterium-mediated transformation driven by either constitutive maize ubiquitin promoter or stress-inducible rd29A...
To observe the effects of Xuesetong Soft Capsules (, Notoginseng total saponin) on angiogenesis and vascular endothelial growth factor (VEGF) mRNA expression in ischemic myocardium rats with myocardial infarction. The left coronary artery was ligated to establish animal model acute Rats were randomly divided into Capsule, Shexiangbaoxin Pill (positive control), (negative control) sham operation groups. After 6 weeks, microvessel count (MVC), density (MVD) VEGF evaluated. MVC MVD infarct...
Disease resistance genes encoding proteins with nucleotide binding sites and Leucine-Rich Repeat (NB-LRR) domains include many members involved in the effector-triggered immunity pathway plants. The transcript levels of these defense are negatively regulated by diverse microRNAs (miRNAs) angiosperms gymnosperms. In wheat, using small RNA expression datasets degradome datasets, we identified five miRNA families targeting NB-LRR monocots, some which arose Triticeae species era. These miRNAs...