Deregulations of long non-coding RNAs (lncRNAs) have been implicated in cancer initiation and progression. Current methods can only capture differential expression lncRNAs at the population level ignore heterogeneous individual patients.We propose a method (LncRIndiv) to identify differentially expressed (DE) patients by exploiting disrupted ordering levels each disease sample comparison with stable normal ordering. LncRIndiv was applied lncRNA profiles lung adenocarcinoma (LUAD). Based on...

The adjuvant chemotherapy, such as cisplatin, doxorubicin, and methotrexate has significantly improved survival of osteosarcoma patients. However, the chemoresistance which arose with chemotherapy blocks achieving favorable outcomes for some patients finally led to relapse or metastatic disease. Studies have shown paradoxical functions autophagy in tumor development, been demonstrated by microRNAs. In present study, we determined involvement during cell line, U-2 OS, further regulation...

Oxidative stress is considered to be an important cause of dysfunction in chondrocytes and articular cartilage degradation, which leads the pathogenesis osteoarthritis (OA) aging. The present study aimed assess effects widely applied antioxidant, ascorbic acid (AA), on human against hydrogen peroxide (H2O2) vitro. Using annexin V‑fluorescein isothiocyanate, 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide senescence‑associated β‑galactosidase assays, identified that AA reduced...

Cardiovascular disease (CAD) is a major global public health issue, with mortality rates being significantly impacted by cold temperatures. Stable and reliable electrocardiogram (ECG) monitoring in environments crucial for early detection treatment of CAD. However, existing skin sensor struggle to balance freeze resistance, breathability, flexibility, conductivity adhesion at Here, we introduce solvent cross-linking strategy an situ transfer method prepare ultrathin bioionic gels, featuring...

Abstract Carbon aerogels with exceptional electrical properties are considered promising materials for bioelectronics in signal detection and stimulation. To address the mechanical incompatibilities of carbon bio‐interfaces, particularly dynamic tissues organs, incorporation hydrogels is an effective strategy. However, achieving excellent performance aerogel‐hydrogel hybrids remains a significant challenge. Two key factors contribute to this difficulty: 1) unrestricted hydrogel infiltration...

To analyze the effect of microRNA-22 on autophagy and proliferation to investigate underlying molecular mechanism osteosarcoma cell chemotherapy sensitivity.MG-63 cells were divided into four groups, including a control group, negative (NC) cisplatin + miR-22 group. Proliferation MG-63 that had been treated with transfected mimics was determined using MTT assay colony formation assay. We assessed degree flow cytometry through cellular staining lysosomal marker monodansylcadaverine (MDC). The...

Copy number variation (CNV) is a kind of chromosomal structural reorganization that has been detected, in this decade, mainly by high-throughput biological technology. Researchers have found CNVs are ubiquitous many species and accumulating evidence indicates closely related with complex diseases. The investigation alterations begun to reveal some important clues the pathologic causes diseases disease process. However, published studies focused on single and, so far, experimental results not...

Abstract Cardiac pacemakers play a crucial role in arrhythmia treatment. Existing devices typically rely on rigid electrode components, leading to potential issues such as heart damage and detachment during prolonged cardiac motion due the mechanical mismatch with tissue. Additionally, traditional pacemakers, their batteries percutaneous leads, introduce infection risks limit freedom of movement. A wireless, battery‐free multifunctional bioelectronic device for pacing is developed. This...

Abstract Background Lung cancer, especially non-small cell lung is a leading cause of malignant tumor death worldwide. Understanding the mechanisms employed by main regulators, such as microRNAs (miRNAs) and transcription factors (TFs), still remains elusive. The patterns their cooperation biological functions in synergistic regulatory network have rarely been studied. Results Here, we describe first miRNA-TF regulation human cancer. We identified important regulators (MYC, NFKB1, miR-590,...

RNA-binding proteins (RBPs) are key regulators of gene expression.

// Yunzhen Wei 1, * , Zhiqiang Chang Cheng Wu 1 Yinling Zhu Kun Li and Yan Xu College of Bioinformatics Science Technology, Harbin Medical University, 150081, China These authors contributed equally to this work Correspondence to: Xu, email: xuyan@ems.hrbmu.edu.cn Keywords: pseudogenes, ceRNA, lung adenocarcinoma Received: July 05, 2017 Accepted: 26, Published: August 04, ABSTRACT Pseudogenes are initially regarded as non-functional genomic fossils resulted from inactivating gene mutations...

The irreproducibility problem seriously hinders the studies on transcriptional signatures for predicting relapse risk of early stage colorectal cancer (CRC) patients. Through reviewing recently published 34 literatures development CRC prognostic based gene expression profiles, we revealed a surprising phenomenon that 33 these analyzed samples with and without adjuvant chemotherapy together in training and/or validation datasets. This data misuse could be partially attributed to unclear...

Pancreatic cancer (PC) with homologous recombination deficiency (HRD) has been reported to benefit from poly ADP-ribose polymerase (PARP) inhibitors. However, accurate identification of HRD status for PC patients the transcriptional level is still a great challenge. Here, based on relative expression ordering (REO)-based algorithm, we developed an signature including 24 gene pairs (24-GPS) using profiles The Cancer Genome Atlas (TCGA). samples classified by 24-GPS showed worse overall...

Hypoxia-inducible factor 1-alpha (HIF-1 α ) and some microRNA (miRNAs) play pivotal roles in response to hypoxia-related physiologic pathophysiologic responses. Up date, the regulatory mechanisms of these molecules were largely unknown chondrocytes. In this study, study degradation homeostasis chondrocytes, effects miRNAs HIF-1 on chondrocytes environment investigated. We found that overexpression miR-210 was present hypoxia C28/I2 human significantly by qRT-PCR western plot. Further...

The incidence and mortality of early-onset colorectal cancer (EOCRC; < 50 years old) is increasing worldwide, with a high recurrence rate. inherent heterogeneity EOCRC makes its treatment challenging. Hence, to further understand the biology reveal molecular mechanisms EOCRC, risk signature needed guide clinical management. Based on relative expression orderings (REOs) genes in each sample, prognostic was developed validated utilizing multiple independent datasets. underlying between...

Protein–protein interaction (PPI) prediction method has provided an opportunity for elucidating potential biological processes and disease mechanisms. We integrated eight features involving proteomic, genomic, phenotype functional annotation datasets by a mixed model consisting of full connected Bayesian (FCB) naive to predict human PPIs, resulting in 40 447 PPIs which contain 2740 common with the protein reference database (HPRD) likelihood ratio cutoff 512. Then we applied them exploring...

5‐Fluorouracil (5‐ FU )‐based adjuvant chemotherapy ( ACT ) is widely used for the treatment of colon cancer. Colon cancers with different primary tumor locations are clinically and molecularly distinct, implied through their response to 5‐ ‐based . In this work, using 69 133 samples patients stage II ‐ III right‐sided left‐sided cancer RCC LCC treated post‐surgery , we preselected gene pairs whose relative expression orderings were significantly correlated disease‐free survival by...

Tannin (TA), as a natural phenolic compound with strong antioxidant activity, has been used feed additive for various animals. In this study, we fed diet containing 800 mg/kg of tannin on

Copy number alteration (CNA) represents an important class of genetic variations that may contribute to tumorigenesis, tumor growth and metastatic spread.

Dosage effect is one of the common mechanisms somatic copy number alteration in development colorectal cancer, yet roles dosage-sensitive genes (DSGs) cancer (CRC) remain to be characterized more deeply. In this study, we developed a five-step pipeline identify DSGs and analyzed their characterization CRC. Results showed that our performed better than existing methods, result was significantly overlapped between solid tumor cell line. We also found top five (PSMF1, RAF1, PTPRA, MKRN2, ELP3)...

The aim of this study was to evaluate the effect anti-osteoporosis treatment on radiological and functional results following anterior cervical discectomy fusion (ACDF).Medical records 59 patients (Mean age 68.1 years, 36 males 23 females) who had ACDF surgery between January 2010 December 2013 were retrospectively reviewed. Antiosteoporotic group consisted 31 non-antiosteoporotic 28 patients. height intervertebral space (HIS), alignment (CA), segmental angle (SA), Visual Analogue Scale...

Several studies have already identified the prognostic markers in colorectal cancer (CRC) based on somatic copy number alteration (SCNA). However, very little information is available regarding their value as a marker. Gene dosage effect one important mechanism of and dosage-sensitive genes are more likely to behave like driver genes. In this work, we propose new pipeline identify CRC. The RNAseq data, CRC from TCGA were assayed screen out SCNAs. Wilcoxon rank-sum test was used...