Photosystem II (PSII), a large multisubunit pigment–protein complex localized in the thylakoid membrane of cyanobacteria and chloroplasts, mediates light-driven evolution oxygen from water. Recently, high-resolution X-ray structure mature PSII has become available. Two polypeptides, D1 CP43, provide many ligands to an inorganic Mn 4 Ca center that is essential for water oxidation. Because its unusual redox chemistry, often undergoes degradation followed by stepwise assembly. Psb27, small...

We report a new mass-spectrometry-based approach for studying protein-folding dynamics on the submillisecond time scale. The strategy couples temperature jump with fast photochemical oxidation of proteins (FPOP), whereby folding/unfolding is followed by changes in oxidative modifications OH radical reactions. Using flow system containing protein barstar as model, we altered protein's equilibrium conformation applying and demonstrated that its reactivity free radicals serves reporter...

Alcohol oxidase I (AOX1) promoter is the most popular but strictly-regulated methanol inducible for heterologous protein expression in Pichia pastoris. In recent years, AOX1 libraries have been developed with deletion or insertion methods. The present research manipulated poly (dA:dT) tracts this to control strength, which hadn't tried before. There were 34 variants derived from native constructed. And integrated into same genomic location and upstream of reporter gene porcine growth hormone...

Abstract Temperature is one of the key environmental factors influencing crop fertility and yield. Understanding how plants sense respond to temperature changes is, therefore, crucial for improving agricultural production. In this study, we characterized a temperature-sensitive male sterile mutant in rice (Oryza sativa), glutamyl-tRNA synthetase 1-2 (ers1-2), that shows reduced at high temperatures restored low temperatures. Mutation ERS1 resulted severely delayed pollen development meiotic...

Dysregulation of transcription is a hallmark cancer, including bladder cancer (BLCA). CRISPR-Cas9 screening using lentivirus library with single guide RNAs (sgRNAs) targeting human factors and chromatin modifiers used to reveal genes critical for the proliferation survival BLCA cells. As result, nuclear factor Y subunit gamma (NFYC)-37, but not NFYC-50, observed promote cell tumor growth in BLCA. Mechanistically, NFYC-37 interacts CBP SREBP2 activate mevalonate pathway transcription,...

ABSTRACT The immobilization of enzymes is one the key issues in both field enzymatic research and industrialization. In this article, we report a facile method for immobilizing Candida antarctica lipase B an alginate carrier. presence calcium cations, enzyme–alginate suspension was crosslinked to form beads with porous structure at room temperature, were well dispersed beads. chiral resolution α‐phenyl ethanol organic phase tested by effects reaction parameters, such as enzyme concentration,...

In the realm of Medical Visual Language Models (Med-VLMs), quest for universal efficient fine-tuning mechanisms remains paramount, especially given researchers in interdisciplinary fields are often extremely short training resources, yet largely unexplored. Given unique challenges medical domain, such as limited data scope and significant domain-specific requirements, evaluating adapting Parameter-Efficient Fine-Tuning (PEFT) methods specifically Med-VLMs is essential. Most current PEFT on...

In the present of study, we have reported complete mitochondrial DNA sequence hybrid Pelteobagrus fulvidraco (♀) × vachelli (♂) that is obtained by artificial hybridization. The total length genome 16,527 bp, with base compositions 30.84% A, 25.54% T, 28.22% C, and 15.40% G. It contains two ribosomal RNA genes, 13 protein-coding 22 transfer a major non-coding control region (D-loop region). arrangement these genes same as observed in teleosts. All protein initiation codons are ATG, except...

Grafting is an important agricultural practice to control soil-borne diseases, alleviate continuous cropping problems and improve stress tolerance in vegetable industry, but it relatively less applied pepper production. A recent study has revealed the key roles of β-1, 4-glucanase graft survival. We speculated that GH9 family gene encoding glucanase may be involved obstacles grafting. Therefore, we performed a systematic analysis pepper, tomato tobacco. total 25, 24 42 genes were identified...

CTX-M-199, a novel chimeric β-lactamase which mediated resistance to sulbactam and tazobactam, was recently identified in Hangzhou, China. This study investigated the prevalence of fecal carriage bacteria producing CTX-M-199 other CTX-M-1/9/1-type enzymes among healthy individuals characterized genetic features bla CTX–M– 1/9/1 -bearing mobile elements. A total 74 Enterobacterales strains carrying various genes, including 64 ( n = 40, rate 0.74%), 199 23, 0.40%), 123 5, 0.10%), 153 CT X–M–...

Uracil-DNA glycosylase (UDG) is one of the most important base excision repair (BER) enzymes involved in uracil-induced DNA lesion by removing uracil from damaged DNA. Uracil may occur due to cytosine deamination or deoxy uridine monophosphate (dUMP) residue misincorporation during synthesis. Medical evidences show that an abnormal expression UDG related different types cancer, including colorectal lung and liver cancer. Therefore, research crucial cancer treatment prevention as well other...

Abstract Alternative polyadenylation (APA) is emerging as a crucial regulatory mechanism in bladder cancer (BC), while it remains elusive whether APA influences the tumor immune microenvironment (TIME) BC. We identified two distinct subtypes of BC by APA-related genes expression profiles. The have different pathological grades, prognostic outcomes, infiltration characteristics, and pathway enrichment. Subsequently, CPSF3 was potential infiltration-related gene Highly expressed positively...

The complete mitochondrial genome of Pseudobagrus vachelli has been sequenced. is 16 529 bp in length, with the base composition 31.61% A, 26.88% T, 26.55% C, and 14.96% G, containing 2 ribosomal RNA genes, 13 protein-coding 22 transfer genes a major non-coding control region (D-loop region). gene order orientation are similar some typical fish species. data will provide useful molecular information for phylogenetic studies concerning P. its related

As an important passive location method, pedestrian positioning technology plays a large role in indoor scenes and other that cannot be covered by GNSS signals. To enhance the accuracy of mobile phones, geomagnetic matching methods are often used to correct resulting position. However, some methods, such as ICCP, not accurate enough require amount calculation. In meantime, they also lack heading constraint. When magnetic interference is severe, it will have great impact on calculating lead...

Abstract Centromeres consist of highly repetitive sequences that are challenging to map, clone, and sequence. Active genes exist in centromeric regions, but their biological functions difficult explore owing extreme suppression recombination these regions. In this study, we used the CRISPR/Cas9 system knock out transcribed gene Mitochondrial Ribosomal Protein L15 (OsMRPL15), located region rice (Oryza sativa) chromosome 8, resulting gametophyte sterility. Osmrpl15 pollen was completely...

The phase behavior and critical parameters of the CO2 (1) + ethanol (2) dichloromethane (3) ternary system were determined, density mixtures was measured in both subcritical supercritical regions system. isothermal compressibility (KT) under some typical conditions calculated using data. data also Peng−Robinson equation state (PR EOS). results showed that at fixed composition temperature is very sensitive to pressure as approaches critical, bubble, dew pressures (both are close point...

In this study, the mitochondrial genome of Oxyeleotris lineolatus was first determined. The length entire mtDNA sequence 16,522 bp with (A + T) content 53.81%, and it contained 13 protein-coding genes, two rRNAs, 22 tRNAs, a control region. gene order orientation are similar to some typical fish species. data will provide useful molecular information for phylogenetic studies concerning O. its related

SUMMARY The pairing and synapsis of homologous chromosomes are crucial for their correct segregation during meiosis. LINC (Linker Nucleoskeleton Cytoskeleton) complex can recruit kinesin protein at the nuclear envelope, affecting telomere bouquet formation pairing. Kinesin‐1‐like Pollen Semi‐Sterility1 (PSS1) plays a pivotal role in male meiotic chromosomal behavior is essential fertility rice. However, its exact meiosis, especially as involved synapsis, has not been fully elucidated. Here,...

Context-aware emotion recognition (CAER) is a complex and significant task that requires perceiving emotions from various contextual cues. Previous approaches primarily focus on designing sophisticated architectures to extract emotional cues images. However, their knowledge confined specific training datasets may reflect the subjective biases of annotators. Furthermore, acquiring large amounts labeled data often challenging in real-world applications. In this paper, we systematically explore...