A5054141698- Bladder and Urothelial Cancer Treatments
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Cancer-related gene regulation
- Lipid metabolism and biosynthesis
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Airway Management and Intubation Techniques
- Peroxisome Proliferator-Activated Receptors
- Urinary and Genital Oncology Studies
- Restraint-Related Deaths
- Genetic factors in colorectal cancer
- Kidney Stones and Urolithiasis Treatments
- Genomics, phytochemicals, and oxidative stress
- Colorectal Cancer Surgical Treatments
- Polyamine Metabolism and Applications
- Renal and Vascular Pathologies
- Pediatric Urology and Nephrology Studies
- Kruppel-like factors research
- Nitrogen and Sulfur Effects on Brassica
- Ubiquitin and proteasome pathways
Republic of Burundi
2021-2024
Union Hospital
2016-2023
Huazhong University of Science and Technology
2015-2023
Union Hospital
2015
The aim of this study was to elucidate the value putative cancer stem cell markers Musashi-1, ALDH1, Sox2, and CD49f in predicting prognosis cervical squamous carcinoma (CSCC). Real-time PCR immunohistochemistry staining performed examine expression archived specimens CSCC patients with postoperative chemotherapy. Kaplan–Meier analysis Cox proportional hazards model were used assess prognostic impact CSC for overall survival (OS) recurrent-free (RFS). data showed that all increased tissues...
Purpose: Nudix Hydrolase 21 (NUDT21) is a crucial mediator involved in alternative polyadenylation (APA), and this molecule has been reported to be tumor suppressor human cancers.However, neither the role NUDT21 plays bladder cancer (BC) nor mechanisms which are have investigated.Methods: Expression levels of BC were evaluated with real-time PCR, western blotting, immunohistochemistry (IHC).In vitro vivo assays performed investigate function tumorigenesis cells.The TOP/FOP flash reporter...
Histone acetyltransferase binding to ORC1 (HBO1), a histone acetyltransferase, was recently identified as an oncoprotein; however, its role in bladder cancer remains unknown. In this study, we showed that HBO1 highly expressed at both the mRNA and protein levels cancer. expression associated with clinical features of human cancer, including tumor size ( P = 0.018) T 0.007) classifications. Patients higher had shorter recurrence‐free survival time, whereas patients lower better time....
Calcium activated chloride channel A4 (CLCA4), a tumor suppressor, was shown to contribute the progression of several human cancers, while its role in bladder carcinoma remains unclear. In this study, we showed CLCA4 expression down-regulated tissues and cells compared adjacent non-tumor normal urothelial cells. Low correlated with larger size, advanced stage, poor prognosis patients. Overexpression profoundly attenuated proliferation, growth, migratory invasive capabilities cancer cells,...
About 10-20% of patients with bladder cancer (BC) progress to muscle-invasive diseases, which the underlying key molecular events have yet be addressed. Here, we identified poly(A) binding protein nuclear 1 (PABPN1), a general factor alternative polyadenylation (APA), was downregulated in BC. Overexpression and knockdown PABPN1 significantly decreased increased BC aggressiveness, respectively. Mechanistically, provide evidence that preference PABPN1-bound signals (PASs) depends on relative...
Leupaxin (LPXN) is a member of the paxillin protein family. Several studies have reported that LPXN regulates cancer development; however, role in bladder remains unknown.The expression cells and tissues was determined by real-time PCR, western blotting, immunohistochemistry, respectively. The biological cell proliferation, invasion, angiogenesis explored both vitro vivo.LPXN elevated lines compared to adjacent non-tumor normal urothelial cells. High correlated with large tumor size,...
R-spondin 3 (RSPO3) is a secreted protein that associates directly with Wnt/β-catenin signaling. However, its functional contribution and prognostic value in human bladder cancer remain unclear. Here, we showed RSPO3 upregulated tissues cells, high expression of correlates advanced clinicopathological features, poor prognosis disease progression patients. Furthermore, observed ectopic or knockdown profoundly promoted inhibited, respectively, the invasive ability cells. Mechanistically, via...
Bladder cancer (BC) is a common genitourinary malignancy. This study investigated the regulatory effects of an exonic circRNA, circNUDT21, in progression BC. The circNUDT21 level was overexpressed BC tissues and cell lines as compared to normal controls. Overexpression silencing promoted inhibited, respectively, proliferative invasive abilities cells. Mechanistical analysis showed that acted miR-16-1-3p sponge MDM2 potential downstream target miR-16-1-3p. We further verified overexpression...
This study aimed to validate the value of urothelial stem cell (USC) markers ΔNp63, integrin β4, CD47, and CD44v6 in predicting prognosis non-muscle invasive bladder cancer (NMIBC) located different anatomic regions bladder.The reviewed clinicopathologic data 169 patients with NMIBC. Using real-time PCR immunohistochemistry, expression archived specimens NMIBC were validated. Kaplan-Meier analysis Cox proportional hazards model used assess prognostic impact USC for recurrent-free survival...
Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism in cancer development and progression. Poly(A) binding protein nuclear 1 (PABPN1) a gene that encodes abundant protein, binds with high affinity to nascent poly(A) tails, crucial for 3'-UTR (3'-untranslated region) APA. Although PABPN1 has been recently reported as dominant master APA regulator clear cell renal carcinoma (ccRCC), the underlying functional remain unclear genes subject regulation...
It has been reported that intrinsic apoptosis is associated with the progression of bladder cancer (BC). Recent evidence suggests polyribonucleotide nucleotidyltransferase 1 (PNPT1) a pivotal mediator involved in RNA decay and cell apoptosis. However, regulation roles PNPT1 remain largely unclear.The upstream miRNA regulators were predicted by silico analysis. The expression levels evaluated real-time PCR, Western blotting, immunohistochemistry (IHC), while miR-183-5p qPCR BC lines tissues....
Abstract Alternative polyadenylation (APA) is emerging as a crucial regulatory mechanism in bladder cancer (BC), while it remains elusive whether APA influences the tumor immune microenvironment (TIME) BC. We identified two distinct subtypes of BC by APA-related genes expression profiles. The have different pathological grades, prognostic outcomes, infiltration characteristics, and pathway enrichment. Subsequently, CPSF3 was potential infiltration-related gene Highly expressed positively...
Clear cell renal carcinoma (ccRCC) is characterized by high metastasis potential. It of great importance to explore the mechanisms underlying ccRCC and enable development potent therapeutics. The mitochondrial complex I (CI) had been considered play an important role in cancers, but less known ccRCC.We utilized available public databases ccRCC, including single-cell RNA sequencing (scRNA-seq) data GSE73121 Cancer Genome Atlas-kidney clear (TCGA-KIRC). Principal component analysis (PCA)...
Abstract Technique modifications that aim to improve ergonomics of the surgical procedure without repositioning upper tract urothelial carcinoma patients remain a challenge urologists. We offer novel technique perform intraperitoneal laparoscopic single-site radical nephroureterectomy and pelvic lymph nodes dissection/retroperitoneal dissection in supine position. Our is feasible offers significant improvement operative efficiency, particularly with locally advanced disease.
To evaluate the effect of real-time three-dimensional (3D) ultrasonography (US) in guiding percutaneous nephrostomy (PCN).A hydronephrosis model was devised which ureters 16 beagles were obstructed. The divided equally into groups 1 and 2. In group 1, PCN performed using 3D US guidance, while 2 guided two-dimensional (2D) US. Visualization needle tract, length puncture time number times recorded for two groups.In score visualization 3, 7.3 ± 3.1 s one time, respectively. 2, respective...