A5009163259- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Cell death mechanisms and regulation
- Urological Disorders and Treatments
- Virus-based gene therapy research
- Retinoids in leukemia and cellular processes
- Urinary Bladder and Prostate Research
- Testicular diseases and treatments
- Immunotherapy and Immune Responses
- Pediatric Urology and Nephrology Studies
- Ureteral procedures and complications
- Kidney Stones and Urolithiasis Treatments
- Protein Degradation and Inhibitors
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Sperm and Testicular Function
- Renal and related cancers
Wuhan Union Hospital
2006-2020
Union Hospital
2010-2019
Huazhong University of Science and Technology
2010-2019
Guangxi Medical University
2008
First Affiliated Hospital of GuangXi Medical University
2008
Union Hospital
2007
Union Hospital
2007
National Natural Science Foundation of China
2006
Tongji Hospital
2000
Wuhan College
1983
Increasing evidences suggest that circular RNAs (circRNAs) exert crucial functions in regulating gene expression. In this study, we perform RNA-seq and identify 6,154 distinct circRNAs from human bladder cancer normal tissues. We find hundreds of are significantly dysregulated further show circHIPK3, also named cancer-related RNA-2 (BCRC-2), is down-regulated tissues cell lines, negatively correlates with grade, invasion as well lymph node metastasis, respectively. Over-expression circHIPK3...
Circular RNAs (circRNAs) are a new member of noncoding (ncRNAs) that have recently been described as key regulators gene expression. Our previous study had identified the negative correlation between circHIPK3 and bladder cancer grade, invasion, well lymph node metastasis. However, roles circRNAs in cellular proliferation remain largely unknown. We analyzed circRNA high-throughout sequencing from human tissues determined related circRNA-3 (BCRC-3, GenBank: KU921434.1) candidate derived PSMD1...
Background/Aims: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as cell growth, apoptosis and migration. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified several cancers, little is known about its role prostate cancer progression. The aim this study was to detect MEG3 expression clinical tissues, investigate functions the development underlying mechanism. Methods: levels were detected by qRT-PCR both tumor...
Abstract Aberrant expression of long noncoding RNA GAS5 in bladder cancer (BC) cells was identified recent studies. However, the regulatory functions and underlying molecular mechanisms BC development remain unclear. Here, we confirmed that there a negative correlation between level tumor clinical stage. Functionally, overexpression reduced cell viability induced apoptosis T24 EJ cells. Mechanistically, effectively repressed EZH2 transcription by directly interacting with E2F4 recruiting to...
Abstract People who develop bladder cancer frequently succumb to the intractable disease. Current treatment strategies are limited presumably due underlying molecular complexity and insufficient comprehension. Therefore, exploration of new therapeutic targets in remains necessary. Here, we identify that bromodomain-4 protein (BRD4), an important epigenome reader bromodomain extraterminal domain (BET) family member, is a key upstream regulator enhancer zeste homologue 2 (EZH2), represents...
Recent studies have demonstrated that long noncoding RNAs (lncRNA) important roles in cancer biology, and the downregulation of lncRNA growth arrest-specific transcript 5 (GAS5) has been reported a variety human cancers. However, its role prostate is largely unknown. This study aims to investigate biological underlying mechanism GAS5 on proliferation cancer. The results demonstrate expression significantly decreased cells compared with epithelial cells. Ectopic inhibited cell induced...
// Wei Xiang 1, * , Jun He Chao Huang 1 Lejun Chen Dan Tao 2 Xinchao Wu Miao Wang Gang Luo Xingyuan Xiao Fuqing Zeng Guosong Jiang Department of Urology, Union Hospital, Tongji Medical College, Huazhong University Science and Technology, Hubei Province, Wuhan 430022, China Oncology, The Fifth Hospital Wuhan, 430050, These authors have contributed equally to this work Correspondence to: Jiang, e-mail: jiangguosongdoc@hotmail.com Zeng, zengfuqingpro@hotmail.com Keywords: clear cell renal...
Renal cell carcinoma (RCC) remains an intractable genitourinary malignancy. Resistance to chemotherapy or targeted therapies in RCC is presumably due the complicated underlying molecular mechanisms and insufficient understanding. The aim of this research was assess expression role bromodomain-4 protein (BRD4) evaluate effects BRD4 inhibitor JQ1 for treatment.BRD4 expressionlevels were assessed by qRT-PCR western blot tissues cells. knockdown on cells MTT assay flow cytometry. vivo treatment...
Background and Purpose Gambogic acid ( GA ) methyl jasmonate MJ are increasingly being recognized as novel natural anticancer compounds. Here, we investigated the antitumour effects of in combination with on human bladder cancer cells. Experimental Approach Cell viability was detected by cell counting kit‐8 assay. apoptosis assessed H oechst 33258 staining flow cytometry. Protein levels were determined immunoblotting expressions mRNA miRNAs RT‐PCR . Differential a group downstream genes...
Background/Aims: Excess fibrosis may lead to chronic pain, scarring, and infertility as endometriosis develops progresses. The pathogenesis of has been linked transforming growth factor-β (TGF-β), the most potent promoter fibrosis. Methods: Levels NR4A1 P-NR4A1 protein in human endometrial endometriotic tissue were assessed by western blotting immunohistochemistry. expression levels fibrotic markers stromal cells evaluated real-time PCR. degree mouse lesions was detected Masson trichrome...
Heparanase (HPA), an endo-h-D-glucuronidase that cleaves the heparan sulfate chain of proteoglycans, is overexpressed in majority human cancers. Recent evidence suggests small interfering RNA (siRNA) induces transcriptional gene silencing (TGS) cells. In this study, transfection siRNA against −9/+10 bp (siH3), but not −174/−155 (siH1) or −134/−115 (siH2) region relative to transcription start site (TSS) locating at 101 upstream translation site, resulted TGS heparanase prostate cancer,...
// Wei Xiang 1, 2 , Xinchao Wu 1 Chao Huang Miao Wang Xian Zhao Gang Luo Yawei Li Guosong Jiang Xingyuan Xiao Fuqing Zeng Department of Urology, Union Hospital, Tongji Medical College, Huazhong University Science and Technology, Wuhan 430022, Hubei Province, PR China No.1 Correspondence to: Xiao, email: xiaoxingyuan2007@gmail.com Zeng, fuqingzengpro@126.com Keywords: bladder cancer, PTTG1, miR-146a-3p Received: April 15, 2016 Accepted: November 11, Published:...
We combine the telomerase extension reaction and microRNA (miRNA)-induced rolling circle amplification, followed by graphene oxide (GO) nicking enzyme-assisted signal amplification as a method to analyze miRNA-21 in urine samples with following merits. First, it is binary assay can simultaneously output double signals that correspond quantities of miRNA, respectively. Second, activity enhanced using DNA molecular beacon probe inhibit formation G-quadruplex. Third, background noise decreased...
Abstracttromal cell-derived factor-1 (SDF-1)/CXCR4 pathway has been shown to play an important role in prostate cancer (PCa) metastasis and siRNA expression using cell-specific promoters demonstrated be a potential tool for targeted gene therapy. Here, we illustrate that human telomerase reverse transcriptase (hTERT) promoter-induced CXCR4 knockdown inhibits PCa bone metastasis. We first investigated expressions interactions of CXCR4/SDF-1 cells, developed retrovirus system could stably...
Special AT-rich sequence-binding protein-1 (SATB1) has been reported to be aberrantly expressed in various cancers and correlated with the malignant behavior of cancer cells. However, function SATB1 RCC remains unclear. With combination immunohistochemistry, western blotting, immunofluorescence, qRT-PCR, cell proliferation, migration invasion assays, we found that levels mRNA protein were dramatically increased human ccRCC tissues (P<0.001 for both), upregulation was significantly associated...