A5052597767- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Bladder and Urothelial Cancer Treatments
- Advanced Data Storage Technologies
- Caching and Content Delivery
- Cancer-related gene regulation
- FOXO transcription factor regulation
- RNA Research and Splicing
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Distributed and Parallel Computing Systems
- Protein Degradation and Inhibitors
- Proteoglycans and glycosaminoglycans research
- Nuclear Receptors and Signaling
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- Cancer, Lipids, and Metabolism
- Cloud Computing and Resource Management
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- RNA regulation and disease
Union Hospital
2016-2025
Huazhong University of Science and Technology
2016-2025
Wuhan Union Hospital
2011-2025
Huanggang Normal University
2010-2024
Union Hospital
2023
China Energy Engineering Corporation (China)
2022
New York University
2016-2020
Communication University of China
2013
Hangzhou Vocational and Technical College
2006-2008
Wuhan National Laboratory for Optoelectronics
2008
Increasing evidences suggest that circular RNAs (circRNAs) exert crucial functions in regulating gene expression. In this study, we perform RNA-seq and identify 6,154 distinct circRNAs from human bladder cancer normal tissues. We find hundreds of are significantly dysregulated further show circHIPK3, also named cancer-related RNA-2 (BCRC-2), is down-regulated tissues cell lines, negatively correlates with grade, invasion as well lymph node metastasis, respectively. Over-expression circHIPK3...
Abstract Cisplatin (CDDP)-based chemotherapy is the first-line treatment for muscle-invasive and metastatic bladder cancer, yet most patients rapidly develop resistance. N6-methyladenosine (m6A) methylation a pervasive RNA modification, its specific role potential mechanism in regulation of CDDP chemosensitivity cancer remain unclear. Furthermore, studies have not fully elucidated whether circular (circRNA) can directly regulate m6A modification mRNA. Here we report upregulation novel...
Background/Aims: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as cell growth, apoptosis and migration. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified several cancers, little is known about its role prostate cancer progression. The aim this study was to detect MEG3 expression clinical tissues, investigate functions the development underlying mechanism. Methods: levels were detected by qRT-PCR both tumor...
Abstract Aberrant expression of long noncoding RNA GAS5 in bladder cancer (BC) cells was identified recent studies. However, the regulatory functions and underlying molecular mechanisms BC development remain unclear. Here, we confirmed that there a negative correlation between level tumor clinical stage. Functionally, overexpression reduced cell viability induced apoptosis T24 EJ cells. Mechanistically, effectively repressed EZH2 transcription by directly interacting with E2F4 recruiting to...
Isorhapontigenin (ISO) is a new derivative of stilbene isolated from the Chinese herb Gnetum cleistostachyum. Our recent studies have revealed that ISO treatment at doses ranging 20 to 80 μM triggers apoptosis in multiple human cancer cell lines. In present study, we evaluated potential effect on autophagy induction. We found sublethal induced effectively bladder cells, which contributed inhibition anchorage-independent growth cells. addition, our ISO-mediated induction occurred SESN2...
Abstract Background Cisplatin (CDDP) has become a standard-of-care treatment for muscle-invasive bladder cancer (MIBC), while chemoresistance remains major challenge. Accumulating evidence indicates that circular RNAs (circRNAs) are discrete functional entities. However, the regulatory functions as well complexities of circRNAs in modulating CDDP-based chemotherapy yet to be revealed. Methods Through analyzing expression profile tissues, RNA FISH, circRNA pull-down assay, mass spectrometry...
Recent studies have demonstrated that long noncoding RNAs (lncRNA) important roles in cancer biology, and the downregulation of lncRNA growth arrest-specific transcript 5 (GAS5) has been reported a variety human cancers. However, its role prostate is largely unknown. This study aims to investigate biological underlying mechanism GAS5 on proliferation cancer. The results demonstrate expression significantly decreased cells compared with epithelial cells. Ectopic inhibited cell induced...
// Wei Xiang 1, * , Jun He Chao Huang 1 Lejun Chen Dan Tao 2 Xinchao Wu Miao Wang Gang Luo Xingyuan Xiao Fuqing Zeng Guosong Jiang Department of Urology, Union Hospital, Tongji Medical College, Huazhong University Science and Technology, Hubei Province, Wuhan 430022, China Oncology, The Fifth Hospital Wuhan, 430050, These authors have contributed equally to this work Correspondence to: Jiang, e-mail: jiangguosongdoc@hotmail.com Zeng, zengfuqingpro@hotmail.com Keywords: clear cell renal...
MiR-138 has been shown to be downregulated in various cancers, including head and neck squamous cell carcinoma (HNSCC) clear renal (ccRCC). In the present study, we aimed reveal mechanism of miR-138 induction senescence cells identify its specific target genes. We used qRT-PCR analyze expression levels lines ccRCC samples. The activity β-galactosidase was measured for functional analysis after mimic transfection. To targets miR-138, three types prediction software determine candidate...
Renal cell carcinoma (RCC) remains an intractable genitourinary malignancy. Resistance to chemotherapy or targeted therapies in RCC is presumably due the complicated underlying molecular mechanisms and insufficient understanding. The aim of this research was assess expression role bromodomain-4 protein (BRD4) evaluate effects BRD4 inhibitor JQ1 for treatment.BRD4 expressionlevels were assessed by qRT-PCR western blot tissues cells. knockdown on cells MTT assay flow cytometry. vivo treatment...
Although our most recent studies have identified Isorhapontigenin (ISO), a novel derivative of stilbene that isolated from Chinese herb Gnetum cleistostachyum, for its inhibition human bladder cancer growth, nothing is known whether ISO possesses an inhibitory effect on invasion. Thus, we addressed this important question in current study and discovered treatment could inhibit mouse-invasive development following carcinogen N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) exposure vivo We also...
Abstract Chromatin accessibility plays important roles in revealing the regulatory networks of gene expression, while its application bladder cancer is yet to be fully elucidated. Chloride intracellular channel 3 (CLIC3) protein has been reported associated with progression some tumors, whereas specific mechanism CLIC3 tumor remains unclear. Here, we screened for key genes through identification transcription factor binding site clustered region (TFCR) on basis chromatin and TF motif. was...
Human bladder cancer (BC) is the fourth most common in United States. Investigation of strategies aiming to elucidate tumor growth and metastatic pathways BC critical for management this disease. Here we found that ATG7 expression was remarkably elevated human urothelial carcinoma N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-induced mouse invasive BC. Knockdown resulted a significant inhibitory effect on tumorigenic cells both vitro vivo by promoting p27 inducing cell cycle arrest at G2/M...
Our recent studies demonstrate that X-linked inhibitor of apoptosis protein (XIAP) is essential for regulating colorectal cancer invasion. Here, we discovered RhoGDIβ was a key XIAP downstream effector mediating bladder (BC) invasion in vitro and vivo. We found both expressions were consistently elevated BCs N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-treated mice comparison to tissues from vehicle-treated human the paired adjacent normal tissues. Knockdown attenuated expression reduced...
Muscle-invasive and metastatic bladder cancer have an extremely poor 5-year survival rate of 5%. In comparison, all other cancers (BCs) a 77%. This striking contrast indicates that one the therapeutic kernels for is to elucidate molecular mechanisms underlying its invasiveness metastasis. current study, we demonstrated maternally expressed gene 3 (MEG3) significantly downregulated in human invasive comparison non-invasive cancers, ectopic expression MEG3 dramatically inhibits cells....
Invasive bladder cancer (BC) is one of the most lethal malignant urological tumors. Although miR-200a has been reported as an onco-miRNA that targets PTEN gene in endometrioid carcinoma, its biological significance BC invasion poorly explored. In current study, we found was markedly overexpressed both human tissues and BBN-induced muscle-invasive tissues. We further showed overexpression specifically promoted cell invasion, but not migration, via transcriptional upregulation matrix...
Although several previous studies have reported the implication of various microRNAs (miRNAs) in regulation human bladder cancer (BC) development, alterations and function many miRNAs growth are not explored yet at present. Here, we screened 1,900 known first discovered that miR-411 was one major miRNAs, which down-regulated n-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-induced BCs. This down-regulation also observed BC tissues cell lines. The results from evaluating relationship between...
Fibroblast growth factor receptor (FGFR) family aberrations are common in urothelial cancer. The FGFR tyrosine kinase inhibitor erdafitinib has been approved for locally advanced or metastatic cancer with FGFR2/3 alterations. Despite the initial efficacy of erdafitinib, resistance cannot be avoided. molecular mechanisms underlying have not well investigated. Here, genome-wide CRISPR screen is performed and coatomer protein complex subunit α (COPA) identified as a key target to enhance...