A5102017309- Mast cells and histamine
- Asthma and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Phytochemical compounds biological activities
- Seaweed-derived Bioactive Compounds
- Phytochemistry and Biological Activities
- Allergic Rhinitis and Sensitization
- Neuropeptides and Animal Physiology
- Food Allergy and Anaphylaxis Research
- Natural Compound Pharmacology Studies
- Plant chemical constituents analysis
- Fungal Biology and Applications
- Cancer therapeutics and mechanisms
- Traditional and Medicinal Uses of Annonaceae
- Bioactive natural compounds
- Monoclonal and Polyclonal Antibodies Research
- Natural product bioactivities and synthesis
- Bioactive Compounds and Antitumor Agents
- Electromagnetic Fields and Biological Effects
- Erythropoietin and Anemia Treatment
- Cancer, Hypoxia, and Metabolism
- Pharmaceutical and Antibiotic Environmental Impacts
- Synthesis and Biological Evaluation
- Healthcare and Venom Research
- Sesquiterpenes and Asteraceae Studies
Meijo University
2009-2024
Aichi Medical University
2011-2024
Japan Tobacco (Japan)
2014-2020
Yokohama City University
2019-2020
University of Shizuoka
2010-2013
Aichi University
2004
Inhibition of hypoxia inducible factor prolyl hydroxylase (PHD) represents a promising strategy for the discovery next generation treatment renal anemia. We identified several 5,6-fused ring systems as novel scaffolds PHD inhibitor on basis pharmacophore analysis. In particular, triazolopyridine derivatives showed potent PHD2 inhibitory activities. Examination predominance triazolopyridines in potency by electrostatic calculations suggested favorable π-π stacking interactions with Tyr310....
The discovery and optimization of a series acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) inhibitors based on pyrimido[4,5-b][1,4]oxazine scaffold is described. SAR moderately potent HTS hit was investigated resulting in the phenylcyclohexylacetic acid 1, which displayed good DGAT1 inhibitory activity, selectivity, PK properties. During preclinical toxicity studies metabolite observed that responsible for elevating levels liver enzymes ALT AST. Subsequently, analogues were synthesized to...
Flavonoids inhibit the activity of chemical mediators released from mast cells. Our aim was to investigate effects natsudaidain, a polymethoxyflavone isolated Citrus plants, on cells.We investigated inhibitory which is histamine release, tumour necrosis factor-alpha production and cyclooxygenase-2 expression in Ca ionophore-stimulated rat basophilic leukemia cells (A23187-stimulated RBL-2H3 cells) by spectrofluorometric, ELISA immunoblotting methods.The percent release A23187-stimulated...
We previously demonstrated that some phenylpropanoids are capable of inhibiting activated mast cells. This study evaluated the anti-allergic effects licarin A, a neolignan isolated from various plants, on antigen-stimulated rat cell line.The inhibitory A histamine release, tumour necrosis factor-α (TNF-α) and prostaglandin D2 (PGD2) production, cyclooxygenase-2 (COX-2) expression in dinitrophenyl-human serum albumin (DNP-HSA) basophilic leukemia cells (DNP-HSA-stimulated RBL-2H3 cells), were...
To date, there is limited data on the biological effects of low-intensity pulsed ultrasound (LIPUS) primary malignant bone tumors. The purpose present study was to investigate antitumor LIPUS osteosarcoma cells. cell viability, induction apoptosis, mitochondrial membrane potential and intracellular signaling molecules in LM8 line were investigated. inhibited viability (P=0.0022) (P=0.0019) Flow cytometry analysis terminal deoxynucleotidyl transferase dUTP nick end labeling staining revealed...
We separated and structurally elucidated three new acridone alkaloids (glycomontamine A (1), B (2), C (3)), together with known compounds (glycofoline, kokusaginine dictamnine) from the acetone extract of Glycosmis lanceolata (Blume) D.Dietr. branches collected in Thailand. The were assayed for cell viability using human lung adenocarcinoma line A549, breast T47D, cervix epithelioid carcinoma Hela, acute lymphoid leukaemia NALM-6, dermal fibroblasts. Hela cells treated compound 1 (IC50 17.6...
Two phenylpropanoids, 1-allyl-3,5-dimethoxy-4-(3-methyl-but-2-enyloxy)benzene (4) and 4-allyl-2,6-dimethoxy-3-(3-methyl-2-butenyl)phenol (6), two phytoquinoids, 4R-(-)-illicinone-A (7) 2S,4R-(-)-illicinone-B (8), isolated from plants of the Illicium species significantly inhibited histamine release rat basophilic leukemia (RBL-2H3) cells stimulated with A23187. Furthermore, these compounds caused a decline in TNF-α levels culture supernatants RBL-2H3 following treatment The results indicate...
Aim: We aimed to investigate the synergistic effects of pulsed electromagnetic field (PEMF) and doxorubicin therapy in a mouse osteosarcoma cell line (LM8 cells) vitro. Materials Methods: The PEMF (5 mT, 200 Hz) different durations on proliferative activity LM8 cells were measured by MTT assay. Apoptotic-related factors such as cell-cycle phase, mitochondrial membrane potential, caspase 3/7 investigated using 4’,6-diamidino-2-phenylindole staining apoptosis kits. Identification intracellular...
Abstract The study of the chemical constituents branches and twigs Cratoxylum cochinchinense collected in Singapore led to isolation structural elucidation four new xanthones, named cratoxanthone A (1), B (2), C (3), D (4), together with six known xanthones (5–10) one dihydroanthracenone (11). Eight (including 1 2) 11 were tested for their antiproliferative activity three human carcinoma cell lines (lung adenocarcinoma A549, colorectal Colo205, epidermoid KB) a acute lymphoblastic leukemia...
Quinones may be formed metabolically or abiotically from environmental pollutants and polycyclic aromatic hydrocarbons (PAHs); many are recognized as toxicological intermediates that cause a variety of deleterious cellular effects including mutagenicity. The PAH-o-quinone, 1,2-naphthoquinone (1,2-NQ), exert its genotoxic through interactions with nucleophiles such DNA, however, the mechanisms 1,2-NQ adduct formation still under investigation. With aim to further understand these mechanisms,...
6, 7-Demethylenedesoxypodophyllotoxin (1) was isolated from the seeds of Hernandia ovigera L. (Hernandiaceae) collected in Taiwan, together with several known lignans. This is first example occurrence 1 a natural source. The assignments 13C-nuclear magnetic resonance signals podophyllotoxin analogues this plant were also established by means two-dimensional H-C correlation spectroscopy (COSY) and long range COSY techniques.
Study of the chemical constituents stems Garcinia schomburgkiana Pierre (Guttiferae), collected in Thailand, led to isolation and identification five known compounds two new biphenyl derivatives, schomburgbiphenyl A (1) B (2). Six phenolic isolated from this plant were screened for their cell growth inhibition activity using several human leukemia lines. One compound, oblongifolin C (7), showed significant cytotoxic towards Jurkat, NALM6, K562 HPB-ALL cells.
Study of the chemical constituents Acronychia pedunculata (L.) Miq. (Rutaceae) stems collected in Taiwan led to isolation and identification eight known three new acetophenones, named acrophenone A (1), B (2), C (3). Of them, acrovestone (5), acropyrone (6) acrovestenol (7), which are dimer compounds, strikingly inhibited proliferation human leukemia cell lines.
The pathogenesis and therapy of hypertrophic scar have not yet been established. Our aim was to investigate the antiproliferative antisecretory effects lapachol, isolated from stem bark Avicennia rumphiana Hall. f., on fibroblasts.The lapachol fibroblast proliferation were measured using MTT assay, cell-cycle analyses lactate dehydrogenase assays. type I collagen α-chain (COL1A1), interleukin-6 (IL-6) plasminogen activator inhibitor-1 (PAI-1) mRNA and/or protein levels scar-fibroblasts...