A5011578561- Pharmacogenetics and Drug Metabolism
- Computational Drug Discovery Methods
- Analytical Chemistry and Chromatography
- Biotin and Related Studies
- Bioinformatics and Genomic Networks
- Advanced Biosensing Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Biomedical Text Mining and Ontologies
- Genetic Associations and Epidemiology
- Metabolomics and Mass Spectrometry Studies
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Biochemical Acid Research Studies
- Protein purification and stability
- Molecular Biology Techniques and Applications
- Eicosanoids and Hypertension Pharmacology
- Advanced Proteomics Techniques and Applications
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Metabolism and Genetic Disorders
- Inflammatory mediators and NSAID effects
- Treatment of Major Depression
- Liver Disease Diagnosis and Treatment
National Academy of Sciences of Belarus
2016-2022
Institute of Bioorganic Chemistry
2014-2022
Center for Systems Biology
2014
Digital Proteomics (United States)
2014
There is increasing evidence that proteins function in the cell as integrated stable or temporally formed protein complexes, interactomes. Previously, using model systems we demonstrated applicability of direct molecular fishing on paramagnetic particles for interactomics (Ershov et al. Proteomics , 2012, 12 3295). In present study, have used a combination affinity‐based and subsequent MS investigation human liver involved interactions with immobilized microsomal cytochrome b5 ( CYB 5A),...
The electrochemical properties of cytochrome P450 2C9 (CYP2C9) and polymorphic modifications 2C9*2 (CYP2C9*2) 2C9*3 (CYP2C9*3) were studied. To analyze the comparative electrocatalytic activity, enzymes immobilized on electrodes modified with a membrane-like synthetic surfactant (didodecyldimethylammonium bromide (DDAB)). An adequate choice type electrode was confirmed by cyclic voltammetry cytochromes under anaerobic conditions, demonstrating well-defined peaks reduction oxidation heme...
Biosensor experiments on investigation of interaction between prostacyclin synthase (PGIS) and different proteins the cytochrome P450 monooxygenase systems were perfomed. Interaction PGIS with microsomal (CYP21A2, CYP2E1) mitochondrial (CYP27A1, CYP11B1, CYP11B2, CYP11A1) P450s was detected. Kinetic equilibrium parameters protein complexes formation determined. Data obtained suggest an essential role these hemoproteins in regulation thromboxane A2 biosynthesis.Vypolneny biosensornye...
The electrochemical properties of cytochrome P450 2C9 (CYP2C9) and polymorphic modifications 2C9*2 (CYP2C9*2) 2C9*3 (CYP2C9*3) were studied. To analyze the comparative catalytic activity, enzymes immobilized on electrodes modified with a membrane-like synthetic surfactant (didodecyldimethylammonium bromide (SPE/DDAB). Peaks reduction oxidation heme iron, which confirms adequate choice type electrode, characterize cyclic voltammograms cytochromes under anaerobic conditions. midpoint...
Identification of new protein-protein interactions (PPI) and characterization quantitative parameters complex formation represent one central tasks protein interactomics. This work is a logical continuation the cycle our previous works devoted to study PPIs among components cytochrome P450-dependent monooxygenase system. Using an optical biosensor Surface Plasmon Resonance (SPR biosensor), comparative analysis on determination kinetic equilibrium between membrane-bound hemoprotein b5 with...
The purpose of research — to develop a pharmacogenetic test predict the speed and features metabolism anticonvulsants, antipsychotics, antidepressants, depending on genetically determined activity enzyme systems proteins-transporters drugs. Materials Methods. During 4 years in study included 260 patients with epilepsy schizophrenia, located hospital treatment Centre Mental Health (Minsk). Patients characterized by presence side effects drug-resistance. Results. As result, taking into account...
A multiplex system to detect mutations at loci rs5985, rs1799983, rs5918, rs2243093, rs4673, rs4646994, rs1722009, rs3980933, rs71103505 associated with the development of cardiovascular diseases has been developed. These belong different types - SNP, STR, Ins/Del therefore, minisequencing and fragment analysis technologies were used determine them. Oligonucleotide was performed amplify all in a single reaction format. The technology, comparison analysis, required additional stages sample...
Azole-containing compounds are often co-administrated with different classes of medications, and widely used as pesticides in agriculture. Besides the main action, azole-containing can directly affect human cytochrome P450 system, inhibiting CYP catalytic activity, thereby leading to metabolism disturbances elimination other drugs, causing toxic effect or resulting a complete absence drug used. In context polypharmacy, it is important take into account influence genetic polymorphism CYP2C9...
A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR by minisequencing (SNaPshot analysis). The conditions for carrying out amplification reactions, as well compositions reaction mixtures, were optimized so that analysis was carried all simultaneously. resulting tested showed a high degree reproducibility...