A5087658589- Pharmacogenetics and Drug Metabolism
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Cholinesterase and Neurodegenerative Diseases
- Electrochemical sensors and biosensors
- Peptidase Inhibition and Analysis
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Hormonal Regulation and Hypertension
- Protein purification and stability
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Eicosanoids and Hypertension Pharmacology
- Protein Structure and Dynamics
- Cholesterol and Lipid Metabolism
Institute of Biomedical Chemistry
2015-2025
Russian Academy of Sciences
2002-2016
Academy of Medical Sciences
2009-2014
Center for Systems Biology
2014
Digital Proteomics (United States)
2014
A global protein interactome ensures the maintenance of regulatory, signaling and structural processes in cells, but at same time, aberrations repertoire protein–protein interactions usually cause a disease onset. Many metabolic enzymes catalyze multistage transformation cholesterol precursors biosynthesis pathway. Cancer-associated deregulation these through various molecular mechanisms results pathological accumulation (its precursors) which can be risk factors. This work is aimed...
Isatin (indole-2,3-dione) is an endogenous indole that has a distinct and discontinuous distribution in the brain other mammalian tissues body fluids. Its output increased under conditions of stress anxiety. itself its analogues exhibit wide range pharmacological activities but specific biological targets still are not well characterized. Affinity chromatography Triton X-100 lysates soluble particulate fractions mouse rat whole homogenates on 5-aminocaproyl-isatin-Sepharose followed by...
Renalase (RNLS) is a protein playing different roles inside and outside cells. A 20-mer synthetic peptide corresponding to the human RNLS amino acid sequence 220-239 (RP220) exhibits number of pharmacologically attractive activities in vitro vivo can bind many renal intracellular proteins. The RP220 contains several cleavage sites for extracellular circulating proteases. Here, we investigated interaction model proteins with renalase 224-232, named RP224-232. We also performed affinity-based...
The interaction of inactivated poliovirus vaccine strains with oriented antibodies immobilized to protein A via Fc fragments has been investigated. Using an SPR biosensor, the kinetic and equilibrium parameters attenuated polioviruses Sabin type 1 2, by various methods were determined. strongest was observed between polyclonal strain 2 β-propiolactone, KD = 1.04⋅10-11 M, as well monoclonal formaldehyde, 1.39⋅10-11 M. high-affinity indicates that D-antigen retained its structure after virus...
Ferredoxins are small iron-sulfur proteins and key players in essential metabolic pathways. Among all types, 3Fe-4S ferredoxins less studied mostly due to anaerobic requirements. Their complexes with cytochrome P450 redox partners have not been structurally characterized. In the present work, we solved structures of both from M. tuberculosis-Fdx alone fusion FdxE-CYP143. Our SPR analysis demonstrated a high-affinity binding FdxE CYP143. According SAXS data, same complex is solution. The...
Cytochromes P450 (CYP) are a family of membrane proteins involved in the production endogenous molecules and metabolism xenobiotics. It is well-known that composition can influence activity orientation CYP proteins. However, little known about how affects ligand binding properties CYP. In this study, we utilized surface plasmon resonance fluorescence lifetime analysis to examine impact micro-environment on interaction between human microsomal CYP51 (CYP51A1) its inhibitor, luteolin...
There is increasing evidence that proteins function in the cell as integrated stable or temporally formed protein complexes, interactomes. Previously, using model systems we demonstrated applicability of direct molecular fishing on paramagnetic particles for interactomics (Ershov et al. Proteomics , 2012, 12 3295). In present study, have used a combination affinity‐based and subsequent MS investigation human liver involved interactions with immobilized microsomal cytochrome b5 ( CYB 5A),...
The amyloid-β peptide is considered as a key player in the development and progression of Alzheimer’s disease (AD). Although good evidence exists that accumulates inside cells, intracellular brain amyloid-binding proteins remain poorly characterized. Proteomic profiling rat homogenates, performed this study, resulted identification 89 individual proteins, approximately 25% them were we had previously identified specifically binding to isatin, an endogenous neuroprotector molecule. A...
Sea anemones are a rich source of Kunitz-type polypeptides that possess not only protease inhibitor activity, but also Kv channels toxicity, analgesic, antihistamine, and anti-inflammatory activities. Two inhibitors belonging to new Heteractis crispa RG (HCRG) polypeptide subfamily have been isolated from the sea anemone crispa. The amino acid sequences HCRG1 HCRG2 identified using Edman degradation method share up 95% their identity with representatives HCGS multigene derived H. cDNA....
Widespread pathologies such as atherosclerosis, metabolic syndrome and cancer are associated with dysregulation of sterol biosynthesis metabolism. Cholesterol modulates the signaling pathways neoplastic transformation tumor progression. Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) catalyzes one key steps in cholesterol biosynthesis. The fairly low somatic mutation frequency CYP51A1, its druggability, well possibility interfering metabolism cells collectively suggest...
There is evidence that the binding of deprenyl, a monoamine oxidase (MAO) B inhibitor, and other propargylamines to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primarily responsible for their neuroprotective antiapoptotic effects. Thus, GAPDH may be target drugs. Using two independent approaches, radioligand analysis an optical biosensor technique, we demonstrate here also interacts with endogenous, reversible MAO isatin. Deprenyl inhibited both [3H]isatin GAPDH, this enzyme isatin...
We describe an experimental approach for direct molecular fishing of prey protein on the surface two types paramagnetic particles (PMP) having different size and composition. Human microsomal cytochrome b(5) (b(5)) its known partner human P450 3A5 (CYP3A5) were used as bait proteins, respectively. For assessing level unspecific binding background α-fetoprotein (aFP) was used. SPR measurements applied quantitative analysis trapped proteins (CYP3A5 aFP) after PMP. It shown that described...
Protein-protein and protein-ligand interactions play a central role in biochemical reactions, understanding these processes is an important task different fields of biomedical science drug discovery. Proteins often work complex assemblies several macromolecules small ligands. The structural functional description protein-protein (PPI) very for basic-, as well applied research. interface areas protein complexes have unique structure properties, so PPI represent prospective targets new...
Abstract A phospholipid‐containing biochip was created by covalently immobilizing phospholipids on the optical biosensor's aminosilane cuvette and employed to monitor interactions of membrane water‐soluble proteins in cytochrome P450‐containing monooxygenase systems with planary layers dilauroylphosphatidylethanolamine (DLPE) distearoylphosphatidylethanolamine (DSPE), differing acyl chain length. It shown that full‐length proteins—cytochrome P4502B4 (d‐2B4), b5 (d‐b5) NADPH‐cytochrome P450...
We attempted to evaluate the affinity of anionic phospholipids cytochrome c by means surface plasmon resonance (SPR) technique and correlate it with active site alterations peroxidase activity. Our experiments showed a strong interdependence between phospholipid fatty acid saturation degree, structure activity complex. Cytochrome Trp59 fluorescence increase in sequence phosphatidyl choline (PC) → phosphatidylserine (PS) cardiolipin (CL) phosphatidic (PA). The association constant ( K )...
Dehydroepiandrosterone (DHEA), a precursor of steroid sex hormones, is synthesized by 17-alpha-hydroxylase/17,20-lyase (CYP17A1) with the participation microsomal cytochrome b5 (CYB5A) and P450 reductase (CPR), followed sulfation two cytosolic sulfotransferases, SULT1E1 SULT2A1, for storage transport to tissues in which its synthesis not available. The involvement CYP17A1 SULTs these successive reactions led us consider possible interaction DHEA-producing redox partners. Text mining...