A5055353012- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Alzheimer's disease research and treatments
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Electrochemical Analysis and Applications
- DNA and Nucleic Acid Chemistry
- Molecular Biology Techniques and Applications
- Electrostatics and Colloid Interactions
- Trace Elements in Health
- Advanced Proteomics Techniques and Applications
- CRISPR and Genetic Engineering
- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Electrochemical sensors and biosensors
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Plant Pathogenic Bacteria Studies
- Nanopore and Nanochannel Transport Studies
Institute of Biomedical Chemistry
2016-2025
University of Tyumen
2022-2024
Engelhardt Institute of Molecular Biology
2013-2020
Russian Academy of Sciences
2007-2019
Yandex (Russia)
2016-2019
Academy of Medical Sciences
2010-2011
Research Institute of Medical Genetics of Russian Academy of Medical Sciences
1999-2002
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1995-2002
National Institutes of Health
1995-2002
Institute of Macromolecular Chemistry
2002
The final goal of the Russian part Chromosome-centric Human Proteome Project (C-HPP) was established as analysis chromosome 18 (Chr 18) protein complement in plasma, liver tissue and HepG2 cells with sensitivity 10–18 M. Using SRM, we have recently targeted 277 Chr proteins liver, cells. On basis results survey, SRM assays were drafted for 250 proteins: 41 found only tissue, 82 specifically detected depleted 127 mapped both samples. carried out 49 proteins; them successfully registered using...
We report the results obtained in 2012–2013 by Russian Consortium for Chromosome-centric Human Proteome Project (C-HPP). The main scope of this work was transcriptome profiling genes on human chromosome 18 (Chr 18), as well their encoded proteome, from three types biomaterials: liver tissue, hepatocellular carcinoma-derived cell line HepG2, and blood plasma. tissue independently performed using two RNaseq platforms (SOLiD Illumina) also droplet digital PCR (ddPCR) quantitative RT-PCR....
The triggers of late-onset sporadic Alzheimer's disease (AD) are still poorly understood. Impairment protein phosphorylation with age is well-known; however, the role in β-amyloid peptide (Aβ) not studied sufficiently. Zinc-induced oligomerization Aβ represents a potential seeding mechanism for formation neurotoxic oligomers and aggregates. Phosphorylation by Ser8 (pS8-Aβ), localized inside zinc-binding domain peptide, may significantly alter its zinc-induced oligomerization. Indeed, using...
The size-dependent electrophoretic migration and separation of liposomes was demonstrated studied in capillary zone electrophoresis (CZE). were extruded nonextruded preparations consisting phosphatidylcholine/phosphatidylglycerol/cholesterol various ratios ranging from 125 to 488 nm mean diameter. When identical surface charge density subjected CZE Tris-HCl (pH 8) buffers ionic strengths (0.001-0.027), they migrated order their size. Size-dependent can be enhanced or brought about by...
To better understand the electrochemical behavior of deoxyribonucleic acid (DNA) on carbon electrodes, oxidation synthetic oligonucleotides 4 to 72 nucleotides long, their thermally stable duplexes, polymerase chain reaction amplicons 141 base pairs and samples double- single-stranded DNA (dsDNA ssDNA) natural origin was studied screen printed electrodes by square wave cycling voltammetry within potential range 0.5–1.5 V (vs. Ag/AgCl). In voltammograms, ssDNA produced two distinct signals at...
Long-read direct RNA sequencing developed by Oxford Nanopore Technologies (ONT) is quickly gaining popularity for transcriptome studies, while fast turnaround time and low cost make it an attractive instrument clinical applications. There a growing interest to utilize data unravel activated biological processes responsible disease progression response therapies. This trend of particular precision medicine which aims at single-patient analysis. Here we evaluated whether gene abundances...
To gain insight into the mechanisms of size-dependent separation microparticles in capillary zone electrophoresis (CZE), sulfated polystyrene latex microspheres 139, 189, 268, and 381 nm radius were subjected to CZE Tris-borate buffers various ionic strengths ranging from 0.0003 0.005, at electric field 100—500 V cm−1. Size-dependent electrophoretic migration particles was shown be an explicit function κR, where κ−1 r are thickness double layer (which can derived strength buffer) particle...
A gene-centric approach was applied for a large-scale study of expression products single chromosome. Transcriptome profiling liver tissue and HepG2 cell line independently performed using two RNA-Seq platforms (SOLiD Illumina) also by Droplet Digital PCR (ddPCR) quantitative RT-PCR. Proteome shotgun LC-MS/MS as well selected reaction monitoring with stable isotope-labeled standards (SRM/SIS) cells. On the basis SRM/SIS measurements, protein copy numbers were estimated Chromosome 18 (Chr 18)...