A5074235213- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Retinoids in leukemia and cellular processes
- Cancer-related molecular mechanisms research
- DNA and Nucleic Acid Chemistry
- Genomics and Phylogenetic Studies
- Electrochemical Analysis and Applications
- Plant Pathogenic Bacteria Studies
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Machine Learning in Bioinformatics
- Protein Degradation and Inhibitors
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Bacterial biofilms and quorum sensing
- Identification and Quantification in Food
Institute of Biomedical Chemistry
2016-2025
University of Tyumen
2023
Russian Academy of Sciences
2008-2012
Academy of Medical Sciences
2008
Universität Greifswald
2005
The final goal of the Russian part Chromosome-centric Human Proteome Project (C-HPP) was established as analysis chromosome 18 (Chr 18) protein complement in plasma, liver tissue and HepG2 cells with sensitivity 10–18 M. Using SRM, we have recently targeted 277 Chr proteins liver, cells. On basis results survey, SRM assays were drafted for 250 proteins: 41 found only tissue, 82 specifically detected depleted 127 mapped both samples. carried out 49 proteins; them successfully registered using...
We report the results obtained in 2012–2013 by Russian Consortium for Chromosome-centric Human Proteome Project (C-HPP). The main scope of this work was transcriptome profiling genes on human chromosome 18 (Chr 18), as well their encoded proteome, from three types biomaterials: liver tissue, hepatocellular carcinoma-derived cell line HepG2, and blood plasma. tissue independently performed using two RNaseq platforms (SOLiD Illumina) also droplet digital PCR (ddPCR) quantitative RT-PCR....
Human hepatoma HepG2 cells are used as an in vitro model of the human liver. High-throughput transcriptomic sequencing is advanced approach for assessing functional state a tissue or cell type. However, influence experimental factors, such sample preparation method and inter-laboratory variation, on profile has not been evaluated. The whole-transcriptome was performed using SOLiD platform validated droplet digital PCR. gene expression compared to results obtained with same another laboratory...
Using 2D electrophoresis the protein expression pattern during growth on carbon sources with different impact catabolite repression of phenol degradation was analysed in a derivative Pseudomonas putida KT2440. The cytosolic cells growing or non-repressive substrate pyruvate almost identical, but showed significant differences to that repressive substrates succinate glucose. Proteins, which were mainly expressed presence pyruvate, could be assigned functional groups transport, detoxification,...
Isatin (indole-2,3-dione) is an endogenous regulator, exhibiting a wide range of biological and pharmacological activities. At doses 100 mg/kg above, isatin neuroprotective in different experimental models neurodegeneration. Good evidence exists that its effects are realized via interaction with numerous isatin-binding proteins identified the brain peripheral tissues studied. In this study, we investigated effect single dose administration to mice (100 mg/kg, 24 h) on differentially...
It is well-known that hyaluronic acid (HA) as a component of brain extracellular matrix (ECM) plays pivotal role in the nervous system and involved synaptic plasticity changes vascular cognitive impairment dementia. HA breakdown feature acute stage stroke injury may be detrimental through enhancement inflammatory response. Recent studies have shown knockout mice lacking synthetase demonstrates epileptic phenotype vivo removal leads to delayed development epileptiform activity cultured...
Information about the characteristics of measuring chips according to their storage conditions is great importance for clinical diagnosis. In our present work, we have studied capability detect nanowire biosensors when they are either freshly prepared or been stored one two years in a clean room. Potential DNA oligonucleotides (oDNAs)—synthetic analogues microRNAs (miRNAs) 198 and 429 that associated with development prostate cancer (PCa)—in buffer solution was demonstrated using biosensor...
Although modern biology is now in the post-genomic era with vastly increased access to high-quality data, set of human genes a known function remains far from complete. This especially true for hundreds mitochondria-associated genes, which are under-characterized and lack clear functional annotation. However, advent multi-omics profiling methods coupled systems algorithms, cellular role many such can be elucidated. Here, we report pathways associated TOMM34, Translocase Outer Mitochondrial...
The ring rot of potato caused by the bacterial pathogen Clavibacter sepedonicus is a quarantine disease posing threat to industry worldwide. sensitive and selective detection C. high importance for its effective control. Here, system reported determine viable bacteria in tubers, based on coupling CRISPR/Cas13a nuclease with NASBA (Nucleic Acid Sequence Based Amplification)—the method isothermal amplification RNA. Detection can be conducted using both instrumental non-instrumental (visual...
Studies of induced granulocytic differentiation help to reveal molecular mechanisms cell maturation. The nuclear proteome represents a rich source regulatory molecules, including transcription factors (TFs). It is important have an understanding perturbations at the early stages processes. By applying proteomic quantitative profiling using isobaric labeling, we found that contents 214, 319, 376, 426, and 391 proteins were altered 3, 6, 9, 12, 72 h, respectively, compared 0 h in HL-60...
Amyloid-β peptide (Aβ) plays a central role in Alzheimer's disease (AD) pathogenesis. Besides extracellular Aβ, intraneuronal Aβ (iAβ) has been suggested to contribute AD onset and development. Based on reported vitro Aβ-DNA interactions nuclear localization of iAβ, the interference iA β with normal DNA expression recently proposed as plausible pathway by which can exert neurotoxicity. Employing sedimentation assay, thioflavin T fluorescence, dynamic light scattering we have studied effects...
Transcriptome profiling is widely employed to analyze transcriptome dynamics when studying various biological processes at the cell and tissue levels. Unlike second generation sequencers, which sequence relatively short fragments of nucleic acids, third DNA/RNA sequencers developed by biotechnology companies “PacBio” “Oxford Nanopore Technologies” allow one transcripts as single molecules may be considered potential molecular counters capable measure number copies each transcript with high...