A5001437608- bioluminescence and chemiluminescence research
- Mitochondrial Function and Pathology
- Free Radicals and Antioxidants
- Biochemical effects in animals
- Chemical and Physical Studies
- Photoreceptor and optogenetics research
- Antioxidant Activity and Oxidative Stress
- thermodynamics and calorimetric analyses
- Laser Applications in Dentistry and Medicine
- Lipid Membrane Structure and Behavior
- Redox biology and oxidative stress
- Medical and Biological Ozone Research
- Photosynthetic Processes and Mechanisms
- Electron Spin Resonance Studies
- Adipose Tissue and Metabolism
- Vitamin C and Antioxidants Research
- Connexins and lens biology
- ATP Synthase and ATPases Research
- Metabolomics and Mass Spectrometry Studies
- Nitric Oxide and Endothelin Effects
- Chemical Reactions and Isotopes
- Photodynamic Therapy Research Studies
- Biofield Effects and Biophysics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Chemical Sensor Technologies
Sechenov University
2018-2024
Lomonosov Moscow State University
2015-2024
Pirogov Russian National Research Medical University
2012-2024
Ministry of Health of the Russian Federation
1995-2024
Federal Medical-Biological Agency
2024
A.V. Shubnikov Institute of Crystallography
2010-2022
Russian Academy of Sciences
1987-2022
Federal Scientific Research Centre Crystallography and Photonics
2018-2022
Moscow State University
2009-2019
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
1994-2015
During apoptosis, cytochrome c (cyt c) is released from intermembrane space of mitochondria into the cytosol where it triggers caspase-dependent machinery. We discovered that cyt plays another critical role in early apoptosis as a cardiolipin (CL)-specific oxygenase to produce CL hydroperoxides required for release pro-apoptotic factors [Kagan, V. E., et al. (2005) Nat. Chem. Biol. 1, 223-232]. quantitatively characterized activation peroxidase activity by and hydrogen peroxide. At low ionic...
Activation of peroxidase catalytic function cytochrome c (cyt c) by anionic lipids is associated with destabilization its tertiary structure. We studied effects several phospholipids on the protein structure monitoring (1) Trp59 fluorescence, (2) Fe−S(Met80) absorbance at 695 nm, and (3) EPR heme nitrosylation. Peroxidase activity was probed using substrates protein-derived radicals. activation cyt did not require complete unfolding or breakage bond. The energy changed in parallel stability...
It has recently been shown that nitrosyl complexes of hemoglobin (NO-Hb) are sensitive to low-level blue laser irradiation, suggesting irradiation can facilitate the release biologically active nitric oxide (NO), which affect tissue perfusion. The aim this study was evaluate therapeutic value for local perfusion after surgical intervention. Blood withdrawn from a rat, exposed NO and infused back same rat or used in vitro experiments. In vitro, an increase NO-Hb levels (electron paramagnetic...
Experimental results on laser beam modulation at selective melting (SLM) are presented. The is a possible way to improve the efficiency of SLM process. optical diagnostics shows energy loss up 30%. This could be consequence high thermal gradients in melt pool resulted by Gaussian power density distribution. distribution can changed flat-top one or inverse-Gaussian (donut) one. An experimental stand with 200W source was developed. Twenty single tracks for each three modes were obtained Co-Cr...
Light-absorbing endogenous cellular proteins, in particular cytochrome c, are used as intrinsic biomarkers for studies of cell biology and environment impacts. To sense c against real biological backgrounds, we combined photothermal (PT) thermal-lens single-channel schematic a back-synchronized measurement mode multiplex transient high resolution (ca. 350 nm) imaging mode. These multifunctional PT techniques using continuous-wave (cw) Ar+ laser nanosecond pulsed optical parametric oscillator...
Abstract— Hemolysis induced by irradiation with ultraviolet (UV) light at 254 nm showed a pronounced oxygen effect: under in vacuum, the rate of hemolysis was decreased an order magnitude. Irradiation air but not vacuum caused peroxidation erythrocyte membrane lipids. These results suggest that lipid photoperoxidation is one causative factors UV hemolysis. different wavelengths UV‐induced membranes developed while antioxidant α‐tocopherol directly photooxidized. It shown process photolysis...