A5082898276- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Metabolism and Genetic Disorders
- Endoplasmic Reticulum Stress and Disease
- Receptor Mechanisms and Signaling
- Occupational and environmental lung diseases
- Hippo pathway signaling and YAP/TAZ
- Mitochondrial Function and Pathology
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Chemokine receptors and signaling
- Chronic Obstructive Pulmonary Disease (COPD) Research
NIHR Leicester Cardiovascular Biomedical Research Unit
2024-2025
Glenfield Hospital
2023-2025
University of Leicester
2023-2025
NIHR Leicester Biomedical Research Centre
2023-2024
The University of Texas Medical Branch at Galveston
2008
Despite strong causal associations with cardiovascular and metabolic disorders including coronary artery disease, hypertension, type 2 diabetes, as well a range of other diseases, the exact function protein SVEP1 remains largely unknown. Animal models have been employed to investigate how contributes focus on murine exploring its role in development, cardiometabolic disease platelet biology. In this study, we aimed comprehensively phenotype proteome Svep1 +/- mice compared wild-type (WT)...
Quantitation of proteins using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is complex, with a multiplicity options ranging from label-free techniques to chemically and metabolically labeling proteins. Increasingly, for clinically relevant analyses, stable isotope-labeled (SIL) internal standards (ISs) represent the "gold standard" quantitation due their similar physiochemical properties analyte, wide availability, ability multiplex several peptides. However, purchase SIL-ISs...
In order for mass spectrometry to continue grow as a platform high-throughput clinical and translational research, careful consideration must be given quality control by ensuring that the assay performs reproducibly accurately precisely. particular, throughput required large cohort validation in biomarker discovery diagnostic screening has driven growth of multiplexed targeted liquid chromatography coupled tandem (LC-MS/MS) assays paired with sample preparation analysis multiwell plates....
Rationale Heavy‐labelled internal standards increasingly represent the gold standard for absolute quantitation in mass spectrometry (MS)‐based bottom‐up proteomics. The biggest drawbacks of using these are that they have high costs and lengthy lead times. Methods We describe an efficient, low‐cost optimised method to enable ‘in‐house’ heavy labelling synthetic tryptic peptides quantification tandem LC‐MS/MS spectrometry. Our methodology uses 18O water a trypsin‐catalysed oxygen exchange...