A5039370145- Monoclonal and Polyclonal Antibodies Research
- Genetic Neurodegenerative Diseases
- Pancreatic function and diabetes
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diabetes Management and Research
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- DNA Repair Mechanisms
- Photochemistry and Electron Transfer Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
- Effects and risks of endocrine disrupting chemicals
- Macrophage Migration Inhibitory Factor
- Estrogen and related hormone effects
- Lung Cancer Research Studies
- Bacteriophages and microbial interactions
- Adenosine and Purinergic Signaling
- Protein Interaction Studies and Fluorescence Analysis
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
Twist Bioscience (United States)
2023-2024
The University of Texas Southwestern Medical Center
2021
Southwestern Medical Center
2021
University of Kentucky
2017-2021
Northern Kentucky University
2011-2012
Abstract How cancer cells cope with high levels of replication stress during rapid proliferation is currently unclear. Here, we show that macrophage migration inhibitory factor (MIF) a 3’ flap nuclease translocates to the nucleus in S phase. Poly(ADP-ribose) polymerase 1 co-localizes MIF DNA fork, where activity required resolve and facilitates tumor growth. loss leads mutation frequency increases, cell cycle delays synthesis growth inhibition, which can be rescued by restoring MIF, but not...
CTG•CAG repeat expansions cause at least twelve inherited neurological diseases. Expansions require the presence, not absence, of mismatch repair protein MutSβ (Msh2-Msh3 heterodimer). To evaluate properties that drive expansions, previous studies have tested under-expression, ATPase function or polymorphic variants Msh2 and Msh3, but in disparate experimental systems. Additionally, some destabilize MutSβ, potentially masking effects biochemical alterations variations. Here, human Msh3 was...
Huntington's disease (HD), a neurodegenerative characterized by progressive dementia, psychiatric problems, and chorea, is known to be caused CAG repeat expansions in the HD gene HTT. However, mechanism of this pathology not fully understood. The translesion DNA polymerase θ (Polθ) carries large insertion sequence its catalytic domain, which has been shown allow loop-outs primer strand. As result high levels oxidative damage neural cells Polθ's subsequent involvement base excision repair...
New immune checkpoints are emerging in a bid to improve response rates immunotherapeutic drugs. The adenosine A 2A receptor (A R) has been proposed as target for development due its participation immunosuppression of the tumor microenvironment. Blockade R could restore immunity and, consequently, patient outcomes. Here, we describe discovery potent, selective, and tumor-suppressing antibody antagonist human (hA by phage display. We constructed screened four single-chain variable fragment...
Congenital hyperinsulinism (HI) is a genetic disorder in which pancreatic β-cell insulin secretion excessive and results hypoglycemia that, without treatment, can cause brain damage or death. Most patients with loss-of-function mutations ABCC8 KCNJ11, the genes encoding ATP-sensitive potassium channel (KATP), are unresponsive to diazoxide, only U.S. Food Drug Administration–approved medical therapy require pancreatectomy. The glucagon-like peptide 1 receptor (GLP-1R) antagonist...
Absorption and emission yields for estrone 17β-estradiol were measured in a variety of room temperature solvents. Molar extinction coefficients found to not vary as function solvent, while fluorescence be significantly affected by the polarity hydrogen-bond accepting ability with yield being highest nonpolar, donating solvents, lowest solvent ethyl acetate. Estrone's was factor ten smaller than 17β-estradiol's. Strong excitation wavelength dependences relative amounts between estrone's two...
The fluorescence lifetimes of the estrogens, estrone, 17β-estradiol and 17α-ethinylestradiol, were studied in various solvents. 17α-ethinylestradiol decreased from 4.7 to 0.9 ns as solvent hydrogen bond accepting ability increased, good agreement with other phenolic molecules. Estrone's two bands had distinct lifetimes, 304 nm band having a lifetime shorter than 200 ps, reflecting efficient energy transfer carbonyl group, which ranging 4.4 4.9 depending on solvent. Solvent effects (1) ππ*,...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, escape disease 2019 therapeutics and vaccines, jeopardize public health. To combat SARS-CoV-2 antigenic escape, we developed a rapid, high-throughput pipeline discover monospecific VHH antibodies iteratively develop VHH-Fc-VHH bispecifics capable of neutralizing emerging variants. By panning single-domain phage libraries against ancestral or beta spike proteins, discovered high-affinity with unique...
Abstract Wnt signaling plays an important role in embryonic development and tumorigenesis. These biological effects are exerted by activation of the β-catenin/TCF transcription complex consequent regulation a set downstream genes. Dickkopf WNT Signaling Pathway Inhibitor 1 (DKK1) has been shown to be potent inhibitor via competing binding LRP5/6. DKK1 is tumorigenic multiple cancer types also immunosuppressive MDSCs NK cells. Emerging evidence indicates that involved T cell differentiation...
Abstract Checkpoint inhibitors represent a major advance in cancer immunotherapy, and combinatorial immunotherapies with secondary drivers of anti-tumor immunity provide beneficial effects for patients that do not show strong endogenous immune response. CD40 is member the TNF family receptors has been shown to play crucial role enhancing B cell dendritic activity fostering responses. Engagement inhibitory Fcg-receptor (FcgR) IIb an absolute requirement vivo antitumor agonistic anti-CD40...
<p>Congenital hyperinsulinism (HI) is a genetic disorder in which pancreatic beta cell insulin secretion excessive and results hypoglycemia that can cause brain damage or death without treatment. Most patients with loss-of-function mutations <em>ABCC8</em> <em>KCNJ11</em>, the genes encoding ATP sensitive potassium channel (KATP), are unresponsive to diazoxide, only FDA approved medical therapy, require pancreatectomy. The glucagon-like peptide 1 receptor...
<p>Congenital hyperinsulinism (HI) is a genetic disorder in which pancreatic beta cell insulin secretion excessive and results hypoglycemia that can cause brain damage or death without treatment. Most patients with loss-of-function mutations <em>ABCC8</em> <em>KCNJ11</em>, the genes encoding ATP sensitive potassium channel (KATP), are unresponsive to diazoxide, only FDA approved medical therapy, require pancreatectomy. The glucagon-like peptide 1 receptor...
Abstract Wnt signaling plays an important role in embryonic development and tumorigenesis. These biological effects are exerted by activation of the β-catenin/TCF transcription complex consequent regulation a set downstream genes. DKK1 has been shown to be potent inhibitor via competing binding LRP5/6. is tumorigenic multiple cancer types also immunosuppressive MDSCs NK cells. Emerging evidence indicates that involved T cell differentiation induction evasion immune surveillance accumulating...