A5057568759- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Neural Networks and Applications
- Ionosphere and magnetosphere dynamics
- Magnetic confinement fusion research
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Non-Destructive Testing Techniques
- Photosynthetic Processes and Mechanisms
- Gene expression and cancer classification
- Fluid Dynamics and Turbulent Flows
- Plasma and Flow Control in Aerodynamics
- Allergic Rhinitis and Sensitization
- interferon and immune responses
- Aerodynamics and Acoustics in Jet Flows
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Manufacturing Process and Optimization
- Entomological Studies and Ecology
- Psychology of Moral and Emotional Judgment
- Microtubule and mitosis dynamics
- Sports injuries and prevention
- RNA regulation and disease
Hiroshima University
2002-2025
Research Organization of Information and Systems
2024
The University of Tokyo
2004-2022
Hiroshima University of Economics
2022
Tokyo University of Science
1977-2019
RIKEN Advanced Science Institute
2016
Hokkaido University
2015
Ishinomaki Senshu University
2010
National Institute of Advanced Industrial Science and Technology
2009-2010
Tokyo Metropolitan University
2010
Incomplete flagellar structures were detected in osmotically shocked cells or membrane-associated fraction of many nonflagellate mutants Salmonella typhimurium by electron microscopy. The predominant types these the cistron specific. incomplete basal bodies flaFI, flaFIV, flaFVIII, and flaFIX mutants, structure homologous to a body flaFV polyhook-basal complex flaR hook-basal flaL flaU mutants. No bases their parts early-fla group flaAI, flaAII, flaAIII, flaB, flaC, flaD, flaE, flaFII,...
Escherichia coli mutants with defects in 29 flagellar genes identified so far were examined by electron microscopy for possession of incomplete structures membrane-associated fractions. The results are discussed consideration the known transcriptional interaction genes. Hook-basal body detected flaD, flaS, flaT, flbC, and hag mutants. flaE mutant had a polyhook-basal structure. An intact basal appeared flaK Putative precursors flaM, flaU, flaV, flaY. No homologous to or its parts flaA, flaB,...
Base-editing technologies, particularly cytosine base editors (CBEs), allow precise gene modification without introducing double-strand breaks; however, unintended RNA off-target effects remain a critical concern and are understudied. To address this gap, we developed PiCTURE, standardized computational pipeline for detecting quantifying transcriptome-wide CBE-induced events. PiCTURE identifies both canonical ACW (W = A or T/U) motif-dependent non-canonical off-targets, revealing broader WCW...
Base-editing technologies, particularly cytosine base editors (CBEs), allow precise gene modification without introducing double-strand breaks; however, unintended RNA off-target effects remain a critical concern and are under studied. To address this gap, we developed the Pipeline for CRISPR-induced Transcriptome-wide Unintended Editing (PiCTURE), standardized computational pipeline detecting quantifying transcriptome-wide CBE-induced events. PiCTURE identifies both canonical ACW (W = A or...
Abstract Genome editing (GE) is widely recognized as an effective and valuable technology in life sciences research. However, certain genes are difficult to edit depending on some factors such the type of species, sequences, GE tools. Therefore, confirming presence or absence practices previous publications crucial for designing establishment research using GE. Although Editing Meta-database (GEM: https://bonohu.hiroshima-u.ac.jp/gem/) aims provide comprehensive information possible, it does...
Flagellar filaments were reconstituted by polymerization with exogenously supplied flagellin monomers at the tips of normal hooks on Salmonella cells which missing because mutations in either flaL or flaU gene genes H1 and H2. Reconstitution did not occur polyhooks flaR mutant cells. Thus, absence flagellar was probably caused inability to work as nuclei for flagellin. A Phf+ produced isolated from a polyhook mutant. Genetic analysis showed that it carried an intracistronic suppressor...
The materials around us usually exist as mixtures of constituents, each constituent with possibly a different elasto-viscoplastic property. How can we describe the material property such mixture is core question this paper. We propose nonlinear blending model that capture intriguing flowing behaviors differ from individual constituents (Fig. 1). used laboratory device, rheometer , to measure properties various fluid-like foods, and found an elastic Herschel-Bulkley has nice agreements...
Due to the importance of physical and chemical properties mold flux used in production high-quality steel, particular suppression surface defects on steel sheets, steelmaking engineers have attempted develop new types flux. This paper presents results research entrapment heat transfer between solidified shell. The authors been developing a with non-Newtonian fluid using nitrogen. That is, viscosity molten is low at high shear rate reduce friction shell, but prevent entrapment. In order...
Nine temperature-sensitive nonflagellate mutants defective in flaFV were isolated from a strain of Salmonella typhimurium. Among them three found to produce flagella with abnormally shaped (either straight or irregularly curved) hooks at the permissive temperature. Two mutations that rendered located one eight segments defined by deletion mapping. The mutation curved was different segment. An flaR introduced into latter mutant. At temperature, resulting double mutant produced polyhooks whose...
Human disease-associated gene data are accessible through databases, including the Open Targets Platform, DisGeNET, miRTex, RNADisease, and PubChem. However, missing entries in such databases anticipated because of curational errors, biases, text-mining failures. Additionally, extensive research on human diseases has led to challenges registering comprehensive data. The lack essential hinders knowledge sharing should be addressed. Therefore, we propose an analysis pipeline explore...
Global transcriptional responses to dehydration and rehydration were determined in Anabaena sp. PCC 7120. Nearly 300 genes up- or downregulated during both rehydration. While as many 133 showed dehydration-specific downregulation, only 29 upregulation. In contrast, while 13 rehydration-specific 259 The upregulated responded rapidly transiently, whereas those did so gradually persistently. expression of various involved DNA repair, protein folding NAD synthesis, well responding nitrogen...
An electron cyclotron range of frequency (ECRF) program has been initiated to study the local heating and current drive in JT-60U. A 110 GHz was adopted couple fundamental O-mode from low-field side with an oblique toroidal injection angle for drive. Experiments were performed at power ~1.5 MW by using three gyrotrons, each which generated output up ~0.8 3 seconds. strongly peaked T e profile observed central temperature increased ~15 keV when absorbed on axis. The clarified significant...
Analysis of RNA-sequencing (RNA-seq) data is an effective means to analyze the gene expression levels under specific conditions and discover new biological knowledge. More than 74,000 experimental series with RNA-seq have been stored in public databases as 20 October 2021. Since this huge amount accumulated from past studies a promising source insights, we focused on meta-analysis 1783 runs two types stressors: oxidative stress (OS) hypoxia. The collected OS were organized dataset retrieve...
Method names are important for the software development process. It has been shown by some studies that quality of method affects comprehension. In response, approaches evaluate comprehensibility have proposed. However, effectiveness existing is limited because they focus on part names.
Allergen from the house dust mite ( Dermatophagoides sp.) is a major trigger factor of allergic disorders, and its characterization crucial for development specific diagnosis or immunotherapy. Here we report identification novel farinae ) antigen whose primary structure belongs to gelsolin family, group actin cytoskeleton‐regulatory proteins. Isolated cDNA, termed Der f 16, encodes 480 amino acids comprising four‐repeated gelsolin‐like segmental structure, which not seen in conventional...
Our understanding of the physics internal transport barriers (ITBs) is being advanced by analysis and comparisons experimental data from many different tokamaks worldwide. An international database consisting scalar two-dimensional profile for ITB plasmas developed to determine requirements formation sustainment ITBs perform tests theory-based models in an effort improve predictive capability models. Analysis using indicates that: (a) power required form decreases with increased negative...
Multitrichously polar flagellated mutants were isolated from a monotrichously strain of Pseudomonas aeruginosa. The ability the mutant cells to swarm in semisolid media at given gel strengths was increased by multiflagellation. Observations electron microscopy revealed that number flagella produced per cell cycle increased. F116 phage-mediated transduction showed multiflagellation occurred single mutation and sites linked fla cluster this organism.
Cellular innate immune system recognizing pathogen infection is critical for the host defense against viruses. Hepatitis B virus (HBV) a DNA with unique life cycle whereby and RNA intermediates present at different phases. However, it still unclear whether viral or templates are recognized by pattern-recognition receptors (PRRs) to trigger antiviral response. Here in this article, we review recent advances progress of HBV studies, focusing on nucleic acid sensors pathways involved...
Target genes for a cAMP receptor protein, AnCrpA, were screened using an Anabaena oligonucleotide microarray and real‐time quantitative reverse transcription polymerase chain reaction (RT‐PCR) analysis. Several gene expressions, including some involved in nitrogen fixation, downregulated the ancrpA disruptant when cells grown with nitrate. Electrophoretic mobility shift assays (EMSAs) revealed that AnCrpA bound to 5′ upstream region of nifB , all1439 hesA all5347 hglE coxBII presence cAMP,...
DNA-binding sites for SYCRP1, which is a regulatory protein of the cyanobacterium Synechocystissp. PCC6803, were predicted whole genome sequence by estimating changes in binding free energy () SYCRP1 those sites. The values calculated summing DeltaDeltaG derived from systematic single base-pair substitution experiments (symmetrical and cooperative model). Of sites, 23 with value <3.9kcal.mol(-1) located upstream or between ORFs selected as putative SYCRP1. In order to confirm whether...
We previously characterized a 177-kDa allergen, M-177, from Dermatophagoides farinae. Thereafter, counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 3, are digested fragments of M-177. The aims this study were clone the cDNA Der f 14 corresponding elucidate allergenic capacities N-terminal fragment (Der 14-N). Recombinant allergens produced trigger-factor-fused proteins in Escherichia coli. 14-N showed highest...
In the presence of p-fluorophenylalanine, a normally flagellated strain Pseudomonas aeruginosa produced straight flagellar filaments at distal ends preexisting flagella, indicating polar growth on its flagella.