A5055767353- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Metabolomics and Mass Spectrometry Studies
- Animal Genetics and Reproduction
- Explainable Artificial Intelligence (XAI)
- Health, Environment, Cognitive Aging
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Climate Change and Health Impacts
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Genetic Associations and Epidemiology
- interferon and immune responses
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Machine Learning in Bioinformatics
- Thermoregulation and physiological responses
- Cancer-related molecular mechanisms research
- Machine Learning in Healthcare
- RNA Research and Splicing
- Genomics, phytochemicals, and oxidative stress
Hiroshima University
2023-2025
Base-editing technologies, particularly cytosine base editors (CBEs), allow precise gene modification without introducing double-strand breaks; however, unintended RNA off-target effects remain a critical concern and are understudied. To address this gap, we developed PiCTURE, standardized computational pipeline for detecting quantifying transcriptome-wide CBE-induced events. PiCTURE identifies both canonical ACW (W = A or T/U) motif-dependent non-canonical off-targets, revealing broader WCW...
Base-editing technologies, particularly cytosine base editors (CBEs), allow precise gene modification without introducing double-strand breaks; however, unintended RNA off-target effects remain a critical concern and are under studied. To address this gap, we developed the Pipeline for CRISPR-induced Transcriptome-wide Unintended Editing (PiCTURE), standardized computational pipeline detecting quantifying transcriptome-wide CBE-induced events. PiCTURE identifies both canonical ACW (W = A or...
Abstract Background Climate change has significantly increased the frequency of exposure to heat, adversely affecting human health and various industrial sectors. Heat stress is an environmental defined as organisms cells abnormally high temperatures. Heat-stress research predominantly focused on response systems involving heat shock factors acting transcription proteins functioning molecular chaperones. However, comprehensively elucidate mechanisms underlying organism’s stress, it essential...