A5074345662- Enzyme Structure and Function
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Adaptive optics and wavefront sensing
- Advanced X-ray Imaging Techniques
- X-ray Spectroscopy and Fluorescence Analysis
- Mass Spectrometry Techniques and Applications
- Advanced optical system design
- Advanced Measurement and Metrology Techniques
European Molecular Biology Laboratory
2017-2025
University of Oxford
2017
Universidad de La Laguna
2008
ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20 keV) and variable-focus (20-200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was last of ESRF Structural Biology Group's beamlines to be constructed commissioned as part ESRF's Phase I Upgrade Program has been user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating situ plate screening capability new flexible sample changer (the FlexHCD) were specifically...
Abstract Serial macromolecular crystallography has become a powerful method to reveal room temperature structures of biological macromolecules and perform time-resolved studies. ID29, flagship beamline the ESRF 4th generation synchrotron, is first synchrotron in world capable delivering high brilliance microsecond X-ray pulses at repetition rate for structure determination temperature. The cardinal combination exposure times, innovative beam characteristics adaptable sample environment...
Automated sample changers are now standard equipment for modern macromolecular crystallography synchrotron beamlines. Nevertheless, most only compatible with a single type of holder and puck. Recent work aimed at reducing sample-handling efforts crystal-alignment times beamlines has resulted in new generation compact precise holders cryocrystallography: miniSPINE NewPin [see the companion paper by Papp et al. (2017, Acta Cryst. , D 73 829–840)]. With full data collection possible within...
Most of the sample holders currently used in macromolecular crystallography offer limited storage density and poor initial crystal-positioning precision upon mounting on a goniometer. This has now become limiting factor at high-throughput beamlines, where data collection can be performed matter seconds. Furthermore, this lack limits potential benefits emerging from automated harvesting systems that could provide crystal-position information which would further enhance alignment beamlines....
A procedure has been developed to compute static aberrations after the PSF measured with lucky imaging technique, using a nearby star as point source probe optical system. This is iteratively turned into phase map at pupil Gerchberg-Saxton algorithm, and then converted adequate actuation information for deformable mirror having low actuator number but large stroke capability. The main advantage of this related capability correcting aberration specific pointing direction without need wavefront sensor.
CrystalDirect is a fully automated crystal harvesting system currently operating at the EMBL Grenoble and Hamburg outstations. The harvester automatically harvests, cryo-cools mounts on sample holders, crystals grown an ultra-thin film directly compatible with X-ray data collection. Here, we report CrystalDirect-to-Beam, proof of concept made ESRF beamline ID30B, where was coupled to changer diffractometer beamline. In this setup, pre-identified provided in crystallization plates are...