A5027015596- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Protein Structure and Dynamics
- RNA modifications and cancer
- RNA Research and Splicing
- CO2 Sequestration and Geologic Interactions
- thermodynamics and calorimetric analyses
- Advanced Chemical Sensor Technologies
- Hydrocarbon exploration and reservoir analysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- HIV Research and Treatment
- CO2 Reduction Techniques and Catalysts
- Atmospheric and Environmental Gas Dynamics
- Viral Infections and Immunology Research
- Nuclear Structure and Function
- ATP Synthase and ATPases Research
- Parasitic Diseases Research and Treatment
- Crystallization and Solubility Studies
- Computational Drug Discovery Methods
Institut pour l'avancée des biosciences
2023-2024
Centre National de la Recherche Scientifique
2011-2024
Université Grenoble Alpes
2016-2024
Inserm
2023-2024
European Molecular Biology Laboratory
2016-2021
Unit of Virus Host Cell Interactions
2016
University of Reading
2016
Architecture et Réactivité de l'arN
2011-2013
Institut de Biologie Moléculaire et Cellulaire
2011-2013
Université de Strasbourg
2011
Isothermal titration calorimetry (ITC) is the method of choice for obtaining thermodynamic data on a great variety systems. Here we show that modern ITC apparatus and new processing methods allow researchers to obtain complete kinetic description systems more diverse than previously thought, ranging from simple ligand binding complex RNA folding. We illustrate these features with case (HIV-1 reverse transcriptase/inhibitor interaction) folding riboswitch triggered by its ligand. The...
Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting processing largely depend on manual operations. Here, a series new methods are presented based low X-ray-background film as support photoablation laser that enable automation major operations required preparation crystals diffraction experiments. In this approach, controlled removal mother liquor before mounting...
Barrier-to-autointegration factor (BAF), encoded by the BANF1 gene, is an abundant and ubiquitously expressed metazoan protein that has multiple functions during cell cycle. Through its ability to cross-bridge two double-stranded DNA (dsDNA), it favours chromosome compaction, participates in post-mitotic nuclear envelope reassembly essential for repair of large ruptures. BAF forms a ternary complex with proteins lamin A/C emerin, interaction defective patients recessive accelerated aging...
Riboswitches are non-coding elements upstream or downstream of mRNAs that, upon binding a specific ligand, regulate transcription and/or translation initiation in bacteria, alternative splicing plants and fungi. We have studied thiamine pyrophosphate (TPP) riboswitches regulating thiM operon thiC E. coli, that THIC the plant A. thaliana. For all, we ascertained an induced-fit mechanism involving initial TPP followed by conformational change leading to higher-affinity complex. The...
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
Prodrugs have little or no pharmacological activity and are converted to active drugs in the body by enzymes, metabolic reactions, through human-controlled actions. However, prodrugs promoting their chemical bioconversion without any of these processes not been reported before. Here, we present an enzyme-independent prodrug activation mechanism boron-based compounds (benzoxaboroles) targeting leucyl-tRNA synthetase (LeuRS), including antibiotic that recently has completed phase II clinical...
The dimerization initiation site (DIS) of the HIV-1 genomic RNA is a conserved stem-loop that promotes viral genome by forming loop-loop complex. DIS constitutes potentially interesting target because it crucial for several key steps replication. In this work we describe synthesis rationally designed aminoglycoside conjugate binds with high specificity, as shown an extensive in vitro binding characterization. We propose three-dimensional model drug-RNA interaction perfectly fits data. Our...
Porphyromonas gingivalis and endodontalis are important bacteria related to periodontitis, the most common chronic inflammatory disease in humans worldwide. Its comorbidity with systemic diseases, such as type 2 diabetes, oral cancers cardiovascular continues generate considerable interest. Surprisingly, these two microorganisms do not ferment carbohydrates; rather they use proteinaceous substrates carbon energy sources. However, underlying biochemical mechanisms of their metabolism remain...
The interplay between humans and their microbiome is crucial for various physiological processes, including nutrient absorption, immune defense, maintaining homeostasis. Microbiome alterations can directly contribute to diseases or heighten likelihood. This relationship extends beyond humans; microbiota play vital roles in other organisms, eukaryotic pathogens causing severe diseases. Notably,
CrystalDirect is a fully automated crystal harvesting system currently operating at the EMBL Grenoble and Hamburg outstations. The harvester automatically harvests, cryo-cools mounts on sample holders, crystals grown an ultra-thin film directly compatible with X-ray data collection. Here, we report CrystalDirect-to-Beam, proof of concept made ESRF beamline ID30B, where was coupled to changer diffractometer beamline. In this setup, pre-identified provided in crystallization plates are...