A5011002662- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- HER2/EGFR in Cancer Research
- RNA and protein synthesis mechanisms
- Galectins and Cancer Biology
- Nitric Oxide and Endothelin Effects
- Protein Structure and Dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Scientific Computing and Data Management
- Adipokines, Inflammation, and Metabolic Diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- DNA and Nucleic Acid Chemistry
- Cytokine Signaling Pathways and Interactions
- HIV Research and Treatment
- Intracranial Aneurysms: Treatment and Complications
- Biochemical and Molecular Research
- Alkaline Phosphatase Research Studies
- Medical Imaging and Pathology Studies
- Diabetes and associated disorders
- Drug Transport and Resistance Mechanisms
- Erythrocyte Function and Pathophysiology
- Advanced Nanomaterials in Catalysis
- Neonatal Health and Biochemistry
- Chemical Synthesis and Analysis
European Molecular Biology Laboratory
2021-2023
BOKU University
2017
Max Perutz Labs
2017
University of Vienna
2017
Proton-dependent oligopeptide transporters (POTs) are promiscuous of the major facilitator superfamily that constitute main route entry for a wide range dietary peptides and orally administrated peptidomimetic drugs. Given their clinical pathophysiological relevance, several POT homologs have been studied extensively at structural molecular level. However, basis recognition transport diverse peptide substrates has remained elusive. We present 14 X-ray structures bacterial DtpB in complex...
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
NADPH oxidases (NOX) are transmembrane proteins, widely spread in eukaryotes and prokaryotes, that produce reactive oxygen species (ROS). Eukaryotes use the ROS products for innate immune defense signaling critical (patho)physiological processes. Despite recent structures of human NOX isoforms, activation electron transfer remains incompletely understood. SpNOX, a homolog from Streptococcus pneumoniae , can serves as robust model exploring transfers family thanks to its constitutive...
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
Fcabs (Fc domain with antigen-binding sites) are promising novel therapeutics. By engineering of the C-terminal loops CH3 domains, 2 antigen binding sites can be inserted in close proximity. To elucidate mode(s) between homodimeric and small antigens, interaction 448 CT6 (having AB, CD EF C-termini engineered) VEGF was investigated. The crystal structures these Fcabs, which form polymers solution, were determined. However, construction heterodimeric (JanusFcabs: one chain Fc-wt,...
Membrane proteins are central to many pathophysiological processes, yet remain very difficult analyze structurally. Moreover, high-throughput structure-based drug discovery has not been exploited for membrane because of lack automation. Here, we present a facile and versatile platform in meso protein crystallization, enabling rapid atomic structure determination at both cryogenic room temperatures. We apply this approach human integral proteins, which allowed us identify different...
Summary Proton-coupled oligopeptide transporters (POTs) are promiscuous of the Major Facilitator Superfamily, that constitute main route entry for a wide range dietary peptides and orally administrated peptidomimetic drugs. Given their clinical pathophysiological relevance, several bacterial mammalian POT homologs have been extensively studied on structural molecular level. However, basis recognition transport peptide substrates has remained elusive. Here we present 14 X-ray structures DtpB...
Summary Membrane proteins are central to many pathophysiological processes yet remain very difficult analyze at a structural level. Moreover, high-throughput structure-based drug discovery has not been exploited for membrane due lack of automation. Here, we present facile and versatile platform in meso protein crystallization, enabling rapid atomic structure determination both cryogenic room temperature single support. We apply this approach two human integral proteins, which allowed us...