A5009051776- Photosynthetic Processes and Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cancer Research and Treatments
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Biochemical and Molecular Research
- Photodynamic Therapy Research Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microbial Community Ecology and Physiology
- Phytochemistry and Bioactivity Studies
- Metal complexes synthesis and properties
- Microbial Metabolism and Applications
- Cassava research and cyanide
- Scientific Computing and Data Management
- Porphyrin Metabolism and Disorders
- Weed Control and Herbicide Applications
- Agriculture and Rural Development Research
- ATP Synthase and ATPases Research
- Diatoms and Algae Research
- Biological Control of Invasive Species
- Allelopathy and phytotoxic interactions
- Synthesis and biological activity
- Pesticide and Herbicide Environmental Studies
European Molecular Biology Laboratory
2020-2022
Institut de Biologie Structurale
2016-2022
Centre National de la Recherche Scientifique
2016-2019
Université Grenoble Alpes
2016-2019
CEA Grenoble
2016-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2019
Laboratoire de Chimie Physique
2016
Université Paris-Sud
2016
Adam Mickiewicz University in Poznań
2014
Until recently, genes coding for homologues of the autofluorescent protein GFP had only been identified in marine organisms from phyla Cnidaria and Arthropoda. New fluorescent-protein have now found phylum Chordata, particularly bright oligomeric fluorescent proteins such as tetrameric yellow lan YFP Branchiostoma lanceolatum . A successful monomerization attempt led to development yellow-green mNeonGreen. The structures mNeonGreen determined compared order rationalize directed evolution...
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
Plant-type L-asparaginases, which are a subclass of the Ntn-hydrolase family, divided into potassium-dependent and potassium-independent enzymes with different substrate preferences. While have already been well characterized, there no structural data for any members group to illuminate intriguing dependence their catalytic mechanism on alkali-metal cations. Here, three crystal structures plant-type L-asparaginase from Phaseolus vulgaris (PvAspG1) differing in type associated alkali metal...
Understanding the mode and site of action a herbicide is key for its efficient development, evaluation toxicological risk, weed control resistance management. Recently, (MoA) cinmethylin was identified in lipid biosynthesis with acyl-ACP thioesterase (FAT) as (SoA). Cinmethylin registered selective use cereal crops grass weeds 2020.Here, we present high-resolution co-crystal structure FAT complex cumyluron by high throughput crystallization screen. We show binding to inhibition cumyluron....
ECFP, the first usable cyan fluorescent protein (CFP), was obtained by adapting tyrosine-based chromophore environment in green to that of a tryptophan-based one. This first-generation CFP superseded popular Cerulean, CyPet, and SCFP3A were engineered rational random mutagenesis, yet latter CFPs still exhibit suboptimal properties pH sensitivity reversible photobleaching behavior. These flaws serendipitously corrected third-generation mTurquoise its successors without an obvious rationale....
EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography services to users worldwide. The HTX lab has strong focus in development of new methods macromolecular crystallography. Through combination crystallization platform, CrystalDirect technology for fully automated crystal mounting and cryocooling CRIMS software we have developed pipelines that can be remotely operated over internet. These include...
Trypanosoma brucei is the causative agent of human African trypanosomiasis (HAT). Nitrogen-containing bisphosphonates, a current treatment for bone diseases, have been shown to block growth T. parasites by inhibiting farnesyl pyrophosphate synthase (FPPS); however, due their poor pharmacokinetic properties, they are not well suited antiparasitic therapy. Recently, an allosteric binding pocket was discovered on FPPS, but its existence trypanosomal FPPS unclear. We applied NMR and X-ray...
Cyan fluorescent proteins (CFPs) are variants of green in which the central tyrosine chromophore has been replaced by a tryptophan. The increased bulk within compact protein and change positioning atoms capable hydrogen bonding have made it difficult to optimize their fluorescence properties, took approximately 15 years between availability first useable CFP, enhanced cyan (ECFP), that variant with almost perfect efficiency, mTurquoise2. To understand molecular bases progressive improvement...
mNeonGreen, an engineered green fluorescent protein (GFP) derived from lancelet, is one of the most brightly homologs Aequorea victoria jellyfish GFP (avGFP) yet reported. In this work, we investigated whether bright fluorescence might be retained in mNeonGreen with modified chromophore structures and altered hues. We found to generally less tolerant than avGFP modification by substitution key chromophore-forming tyrosine residue other aromatic amino acids. However, were ultimately...