A5039619970- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Lipid metabolism and biosynthesis
- Neurobiology and Insect Physiology Research
- Photosynthetic Processes and Mechanisms
- Weed Control and Herbicide Applications
- Cancer-related gene regulation
- Pesticide and Herbicide Environmental Studies
- Endoplasmic Reticulum Stress and Disease
- Parasitic Infections and Diagnostics
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Nuclear Structure and Function
- Allelopathy and phytotoxic interactions
European Molecular Biology Laboratory
2019-2024
Max Planck Institute for Biophysical Chemistry
2014-2020
Understanding the mode and site of action a herbicide is key for its efficient development, evaluation toxicological risk, weed control resistance management. Recently, (MoA) cinmethylin was identified in lipid biosynthesis with acyl-ACP thioesterase (FAT) as (SoA). Cinmethylin registered selective use cereal crops grass weeds 2020.Here, we present high-resolution co-crystal structure FAT complex cumyluron by high throughput crystallization screen. We show binding to inhibition cumyluron....
DNMT1 is an essential DNA methyltransferase that catalyzes the transfer of methyl groups to CpG islands in and generates a prominent epigenetic mark. The catalytic activity relies on its conformational plasticity ability change conformation from auto-inhibited activated state. Here, we present four cryo-EM reconstructions apo DNTM1: non-productive DNA, H3Ub2-peptide, productive complexes. Our structures demonstrate flexibility DNMT1’s N-terminal regulatory domains during transition...
Cohesin is a protein complex whose core subunits, Smc1, Smc3, Scc1, and SA1/SA2 form ring-like structure encircling the DNA. Cohesins play key role in expression, repair, segregation of eukaryotic genomes. Following catalytic mechanism that insufficiently understood, Esco1 Esco2 acetyltransferases acetylate cohesin subunit thereby inducing stabilization on As prerequisite for structure-guided investigation enzymatic activity, we determine here crystal mouse Esco2/CoA at 1.8 Å resolution. We...
Beyond participating in splicing, some spliceosomal components from higher eukaryotes play pivotal roles coupling splicing to other RNA-processing events. The massive helicase Aquarius (170 kDa) is loaded at a specific location on the intron, where it acts as molecular linker between deposition of exon-junction complex mRNA and formation snoRNPs intronic segments. We have determined crystal structures full-length with non-hydrolysable ATP analog well single-stranded RNA. structure reveals...
Abstract Cohesin is a protein complex encircles the DNA and regulates separation of sister chromatids during cell division. Following catalytic mechanism that insufficiently understood, Esco1 Esco2 acetyltransferases acetylate Smc3 subunit cohesin, thereby inducing stabilization cohesin on DNA. As prerequisite for structure-guided investigation enzymatic activity, we determine here crystal structure mouse Esco2/CoA at 1.8 Å resolution. We reconstitute entire as tetrameric assembly use it...