A5056803899- RNA Research and Splicing
- Muscle Physiology and Disorders
- Cardiomyopathy and Myosin Studies
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Genomics and Chromatin Dynamics
- Renal and related cancers
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Reproductive System and Pregnancy
- DNA and Nucleic Acid Chemistry
- Molecular Communication and Nanonetworks
- Pancreatic function and diabetes
- Marine and coastal plant biology
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Photoreceptor and optogenetics research
- Reproductive Biology and Fertility
- Liver physiology and pathology
Université de Bordeaux
2006-2024
Inserm
1988-2024
Bordeaux Population Health
2016-2024
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2016-2018
Centre Hospitalier Universitaire de Bordeaux
2017
Immunology from Concept and Experiments to Translation
2008-2014
Centre National de la Recherche Scientifique
1993-2014
Institut de génétique et de développement de Rennes
1990-1996
Université de Rennes
1990-1996
California Institute of Technology
1988-1990
We investigated the possibility of infecting normal adult human hepatocytes maintained in pure cultures or cocultures with hepatitis B virus (HBV). Several assays different infectious sera and hepatocyte populations from various donors identified only limited HBV replication, significant variations one cell preparation to another. The addition 1.5% dimethyl sulfoxide culture medium markedly enhanced infection process. Indeed, e antigen secretion, appearance both DNA replicative forms major...
Abstract Rhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. Here, we examine properties OLPVR1 (Organic Lake Phycodnavirus Rhodopsin) and two other type 1 viral channelrhodopsins (VCR1s), demonstrate that VCR1s accumulate exclusively intracellularly, and, upon illumination, induce calcium release from...
Previous gene-transfer experiments have identified a 2500-nucleotide 5' domain of the CyIIIa cytoskeletal actin gene, which contains cis-regulatory sequences that are necessary and sufficient for spatial temporal control gene expression during embryogenesis. This is activated in late cleavage, exclusively aboral ectoderm cell lineages. In this study, we focus on interactions demonstrated vitro between regulatory proteins present crude extracts derived from sea urchin embryo nuclei...
The Drosophila Vestigial and Scalloped proteins form heterodimers that control wing development are involved in muscle differentiation. Four vestigial like genes have been described mammals. Similar to the gene, they encode a short conserved domain (TONDU) required for interaction with mammalian paralogues of (i.e., TEAD proteins). We previously identified two Xenopus laevis we report here expression four distinct (vgll1-4) represent amphibian orthologs genes. Vgll1 has unique pattern which...
Abstract Expression of the Cylla cytoskeletal actin gene is a marker differential activation in aboral ectoderm early sea urchin embryo. Gene transfer expreiments have defined 2,300 nucleotide cis‐regulatory domain required for correct spatial and temporal control this gene. This includes at least 20 sites which relatively stable DNA‐protein complexes form vitro on reaction with embryo nuclear extracts. We report sequence whole regulatory map high‐specificity interactions occur. These were...
Drosophila Vestigial is the founding member of a protein family containing highly conserved domain, called Tondu that mediates their interaction with members TEAD transcription factors (Scalloped in Drosophila). In Drosophila, Vestigial/Scalloped complex controls wing development by regulating expression target genes through binding to MCAT sequences. vertebrates, there are four Vestigial-like whose functions still not well understood. Here we describe regulation and function vestigial-like...
WD repeat-containing protein 5 (WDR5) is a common component of mammalian mixed lineage leukemia methyltransferase family members and important for histone H3 lysine 4 methylation (H3K4me), which has been implicated in control activation cell genes during embryogenesis. However, WDR5 not considered to play specific regulatory role epigenetic programming because it ubiquitously expressed. Previous work from our laboratory showed the appearance H3K4me within smooth muscle (SMC)-marker gene...
Abstract Ultraviolet B (UVB) in sunlight cause skin damage, ranging from wrinkles to photoaging and cancer. UVB can affect genomic DNA by creating cyclobutane pyrimidine dimers (CPDs) pyrimidine–pyrimidine (6–4) photoproducts (6–4PPs). These lesions are mainly repaired the nucleotide excision repair (NER) system photolyase enzymes that activated blue light. Our main goal was validate use of Xenopus laevis as an vivo model for investigating impact on physiology. The mRNA expression levels xpc...
We determined the distribution of cis-regulatory sites, previously identified in control domain CyIIIa gene, three other genes displaying diverse spatial patterns expression sea urchin embryo. Competitive gel-shift reactions were carried out using probes from with competitor fragments isolated defined domains genes. is expressed only aboral ectoderm lineages; studied Spec1, also ectoderm; CyI, many different cell types; and SM50, skeletogenic mesenchyme. All four are activated at about same...
In vertebrates, the actin-binding proteins tropomyosins are encoded by four distinct genes that expressed in a complex pattern during development and muscle differentiation. this study, we have characterized transcriptional machinery of alpha-tropomyosin (alpha-Tm) gene cells. Promoter analysis revealed 284-bp proximal promoter region Xenopus laevis alpha-Tm is sufficient for maximal activity three cell types. The types depends on both common cis-regulatory sequences. We identified 30-bp...
Pluripotent mouse embryonic stem cells (mESCs), maintained in the presence of leukemia inhibitory factor (LIF) cytokine, provide a powerful model with which to study pluripotency and differentiation programs. Extensive microarray studies on cultured have led identification three LIF signatures. Here we focus muscle ras oncogene homolog (MRAS), is small GTPase Ras family encoded within Pluri gene cluster. To characterise effects Mras cell differentiation, used gain- loss-of-function...
Diffuse midline glioma (DMG) is a pediatric malignancy with poor prognosis. Most children die less than one year after diagnosis. Recently, mutations in histone H3 have been identified and are believed to be oncogenic drivers. Targeting this epigenetic abnormality using deacetylase (HDAC) inhibitors such as panobinostat (PS) therefore novel therapeutic option currently evaluated clinical trials.BH3 profiling revealed engagement an irreversible apoptotic process of cells exposed PS confirmed...
Transcription enhancer factors 1 (TEF-1 or TEAD) make a highly conserved family of eukaryotic DNA binding proteins that activate not only viral regulatory elements but muscle specific genes and are involved in several developmental processes. In this study, we report the identification expression pattern NTEF-1 (TEAD1) DTEF-1 (TEAD3), two members Xenopus laevis. Both X. laevis (XNTEF-1 XTEAD1) (XDTEF-1 XTEAD3) possess 72 amino acid TEA domain characteristic TEF-1 proteins. XNTEF-1 is 426...
ZFP36 constitutes a small family of RNA binding proteins (formerly known as the TIS11 family) that target mRNA and promote their degradation. In mammals, are encoded by four genes and, although they show similar activities in cellular destabilization assay, there is still limited knowledge targets it not whether or have redundant functions. present work, we used Xenopus embryo, model system allowing gain- loss-of-function studies, to investigate, individual had distinct We overexpression...
Primary cultures of non-proliferating hepatocytes isolated by the two-step collagenase perfusion method from woodchuck naturally infected with hepatitis virus (WHV) were used to study WHV propagation in vitro. Hepatocytes carrying DNA exhibited a very high level survival and retained their morphological characteristics for 2 3 months. Over this time, they found produce virus-specific proteins release viral particles polymerase activity into medium. Using Southern blot analysis recombinant B...