Clb1 and Clb2 are functionally redundant B-type cyclins, the clb1Δ clb2Δ double mutant is lethal. In normal mitotic growth, plays central role in G2-M progression. We previously demonstrated that RNA-binding protein Puf5 positively regulates CLB1 expression by downregulating of repressor Ixr1. The decreased puf5Δ mutation caused a severe growth defect mutant. On contrary, CLB2 was unchanged between wild-type strain unsynchronized cultures, did not show retardation. Therefore, we assumed main...

We fabricated and experimentally investigated a nanostructure known as quantum-well (QW) microtube, which is fine tube with micron- or nanometer-order diameter by rolling semiconductor GaAs QW. Although the wall thickness only 40 nm, system retains quantum properties of QW, photoluminescence from QW subband can be clearly observed. Even though width sufficiently small to make type-II band-aligned, type-I transition caused uniaxial strain in microtube allows for optical emission.

Abstract Ti–Ni–Si glassy alloy supercapacitors, devices that store electric charge on their TiO 2 surfaces contain many nanometer‐sized cavities, display advantages over other power‐source technologies. The use of de‐alloying and anodic oxidization methods has made possible the synthesis a surface accessible to electron trapping. Until recently, no studies have addressed “dry” storage in light alloys. Our device realizes AC from 193 453 K with voltage variation 10 150 V, DC capacitance ∼4.8...

We had developed an electron beam recorder for high-density mastering. The has a capability to record patterning beyond 200 Gbit/in 2 density because it characteristic of fine convergence. behavior scattering is important realizing patterning. Scattered electrons degrade resolution and can not be realized. Thus, we adopted new substrate made material that reduces the influence backscattering attempted high density. As experimental result, 300 could

We realized an inorganic write-once disk using a Bi-Ge nitride alloy recording layer for optical system the Blu-ray disc format. developed system, which is composed of two types metal as material. One decomposed easily by heat laser beam during recording. The other not at that temperature. signal modulation depends mainly on reflectivity change caused decomposition nitride. non-decomposing controlled reaction and prevented deformation layer. Thus we could obtain well-formed minute mark....

We have developed an electron beam recorder and utilized it in high-density disk mastering. discussed scattering mastering that degrades the reproduction performance of disks. In order to improve performance, we applied a dry etching process process, confirmed its efficacy reducing effect improving noise by calculation experiments. fabricated `Blu-ray Disc'-type read-only memory disks with without noticed advantage from jitter comparison. Finally, 4.7% was achieved 25 GB capacity disk, while...

Puf5, a Puf-family RNA-binding protein, binds to 3´ untranslated region of target mRNAs and negatively regulates their expression in Saccharomyces cerevisiae. The puf5Δ mutant shows pleiotropic phenotypes including weakened cell wall, temperature-sensitive growth, shorter lifespan. To further analyze role Puf5 we searched for multicopy suppressor the growth this study. We found that overexpression CLB2 encoding B-type cyclin suppressed mutant. clb2Δ double displayed severe defect, suggesting...

Thin layers of silica-surfactant hybrids with the thickness several tens nm formed on flat substrate from basic aqueous methanol solution containing tetraethoxysilane, behenyltrimethylammonium chloride, and ammonia. From same solution, micrometer sized spherical particles were obtained. Both film transformed to nanoporous silicas by calcination in air. The synthetic variables have been examined find following dominant experimental parameters; pore size silica can be controlled temperature...

Amorphous film of NdDyFeCo alloy has been investigated for high performance magneto-optical disk. Studies on magneto-optic effect and magnetic properties found that a square hysteresis loop coercivity (Nd 13 Dy 87 ) 30 (Fe 84 Co 16 70 were significantly improved, large Kerr-rotation 0.4 degree Curie temperature 150°C obtained. Dynamic characteristics have revealed disk polycarbonate substrate(5.25''-diam. with track pitch 1.6µm) had C/N ratio 58dB 49 dB at 2MHz 5MHz, respectively. This media...

RNA stability control contributes to the proper expression of gene products. Messenger RNAs (mRNAs) in eukaryotic cells possess a 5’ cap structure and 3’ poly(A) tail which are important for mRNA efficient translation. The Ccr4-Not complex is major cytoplasmic deadenylase functions degradation. CLB1-6 genes Saccharomyces cerevisiae encode B-type cyclins involved cell cycle progression together with cyclin-dependent kinase Cdc28. CLB consist CLB1/2 , CLB3/4 CLB5/6 whose products accumulate at...

This paper proposes a software testing support system with the hardware failure simulation functions. In recent years, high integration of semiconductor device is advanced by development smartphone and tablet device. By such reason, CD-SEM which tester using an electron beam needs precisely functionality. Software concurrent are adopted to promote CD-SEM. However, delay have been becoming big issue. One factors that test environment large-scale comprised plural computers insufficient. The...

In this paper, outline of EBR and the results circle patterning were reported. Electron beam recorder with /spl theta/-stage had a promising ability for high accuracy recording.

A digitized phase sensitive detecting system(DPD)have been developed for precision measurement of voltage ratio. The system is composed a personal computer, an analog-to-digital converter(ADC)and software mesurement and data treatment. Two sinusoidal voltages frequency applied to the DPD are converted into discrete data, expressed as vectors by treatment following principle usual detector. Then amplitude ratio difference two input calculated. Estimeted uncertainties measured values less than...

This paper deals with four processes for constructing mathematical models based on measurement data. The first process is smoothing data the hypothesis that a small area of curved surface can be approximated to an ellipsoid. second fitting into cross points between 3D lattice and filling by approximating ellipse surface. As information positions converted from coordinate value integral code, it easier find neighboring clear relations surfaces help prevent gap when surfaces. third recognizing...

Agglomerated anatase TiO2-oyster shell powders were sprayed by high-velocity oxy-fuel (HVOF) spraying on an A5052 (Al-Mg alloy) substrate. The prepared the spray dry method with various mixing ratios of 20%, 40%, and 60%. primary particle size TiO2 was 7 nm, 30 nm 200 nm. Phase transformation from to rutile occurred during HVOF process. A higher ratio could be obtained for nm-agglomerated powder. Therefore, powder used agglomerated mean diameters varying 35μm.Sprayed coatings consisted...

The shape of base plate used for welding test was changed in thickness, width and length independently. A single bead deposited on the center-line each over wholelength with covered electrode under uniform conditions.The thickness influences most severly cooling curve heat-affected zone near fusion line. has also certain effect curve, but scarcely concerns. curves are similar, when plate-size is 11mm larger than 150mm width. maximum hardness inclines to become constant this size.In...

To make clear the effects of welding conditions on micro structure and hardness in heat-affected zone high tensile Mn-Si steel, authors have studied cooling phenomena at that neighbourhood fusion line.According to first report, there was no variation curves hardnesses unless specimens were smaller than 150 mm width under reported conditions. Therefore, we continued investigation effect thickness using certain larger got some relations between curve microstructure part with maximum...

Authors made a series of investigation on structure and hardness heat-affected zone, welding with single-V-butt T-joint which are used generally in industries.In this report the softening effect caused by subsequent neighbourhood first pass was examined. On other hand, an for completely welded specimens case above mentioned joints.In joint, maximum zone observed neighbour-hood last pass, while root heat subsequently passes.In T type fillet little lowered slightly, when microstructure...

This paper deals with four processes for constructing mathematical models based on measurement data. The first process is smoothing data the hypothesis that a small area of curved surface can be approximated to an ellipsoid. second fitting into cross points between 3D lattice and fill by approximating ellipse surface. third recognizing surfaces boundary lines composing physical model curvature located variance coordinate values points. last approximately defining NURBS minimum square method...

This paper deals with two processes for constructing the mathematical models based on measurement data. The first process is to recognize surfaces composing a physical model by curvature measured points or variance of coordinate values points. second approximately define NURBS minimum square method average positional errors. In this study, primitive and complex shapes are used as evaluation model. results show usefulness system proposed methods.

A precise measuring system based on the least squares method is developed for audio frequency voltage. The consists of an analog-to-digital converter and a personal computer. An unkown voltage applied to converted into discrete data with usual sampling or compressed method, precisely expressed as polar vector by means method. Some basic properties system, such accidental errors parameters estimated effect asynchronous characteristics are studied. Thus, it that unknown about 5 Vms measured...