Mammary gland tissue inflammation (mastitis) is the most frequent disease in dairy cattle world, and negatively influences milk quality having consequences for processing industry. The aim of study was analyze designed preventive control methods focused to reduction mastitis herd Slovak pied east Slovakia during two years experiment. From 180 cows at quarterly intervals 3rd, 6th, 9th 12th month performed a complex examination health udder including an assessment clinical signs mammary gland,...

Early identification of mastitis is a serious challenge for dairy farmers and veterinarians in ensuring the health an animal hygienic quality produced milk. The purpose this study was to detect occurrence aetiology herd 153 milked cows localised farm west Slovakia. During complex investigation, 606 quarter milk samples were examined (6 quarters discarded) classified based on clinical status, presence abnormal udder secretions, result California test (CMT), somatic cell count (SCC)...

Abstract An effect of mammary gland infection caused by Streptococcus uberis on the changes in cows’ milk composition and its physicochemical properties was examined. The study conducted herd Slovak Pied breed cattle (with a share HF blood), 2 nd 3rd lactation, after 4 th month milking. Milk samples were collected from quarter subjected to microbiological analysis, basic composition, total bacteria count, somatic cell count Also analyses protein fractions fatty acids profile conducted....

The Aerococcus viridans isolates from bovine mastitis in Slovakia were isolated and characterized by classical microbiological biochemical, molecular techniques including IGS-PCR rep-PCR, ARDRA 16S rDNA gene sequencing. substantial variability of antibiotic resistance patterns was observed. majority strains resistant to beta-lactam antibiotics, the tetracycline observed 3 tested strains, lincomycin found 4 practically all sensitive neomycin ciprofloxacin. While variable at a phenotypic...

Experiment we carried out on the herd of 115 Slovak Pied dairy cows. Were realized three collections individual milk samples for isolation and identification coagulase-negative staphylococci (CNS). We collected 341 samples. Coagulase - negative were isolated in 18.8 % (64). Highest number CNS from acute, subclinical, subacute latent forms mastitis, respectively. S. warneri (2.88 %), chromogenes (17.19 cohnii (15.63 %) xylosus (14.06 was highest percentage. Was detected presence 7 virulence...

In our study was followed occurrence of staphylococcal mastitis in herd 350 sheep during one lactation season. We found, the bacteria S. schleiferi identified 88 from all 204 isolates. high number were also caprae (33), chromogenes (21), aureus (19), epidermidis (17), respectively. Important intermedius, simulans, xylosus a warneri, too. The Staphylococcuss spp. caused latent and subclinical forms predominantly, showed into subacute (26.5%). Acute determined 7.8 %. Eight aureus, two...

The aim of this study was an evaluation the effects two species coagulase negative staphylococci (CNS), Staphylococcus xylosus and warneri, on changes in technological parameters cows' milk. conducted a herd Slovak Pied cattle breed (with share HF blood). Based performance results from three subsequent months, cows 2nd 3rd lactation with SCC up to 200 thousand/ml (8 heads, 32 quarters), above 800 after 4th month lactation, were selected. samples subjected microbiological analysis, total...

In this study we investigated 24 strains of Staphylococcus aureus and 33 epidermidis isolated from milk sheep with clinical mastitis, for their ability to form biofilms. Three methods the determination a biofilm were used. When evaluating growth on Congo Red agar (CRA), 79.2% S. 72.7% positive formation. The quantitative method detection Microtitre Plate (MTP) revealed results 75.0% samples 75.8% samples. Using PCR presence genes that affect formation biofilms, most frequently determined eno...

The aim of the experiment was to study selenium and vitamin E sources in diet dairy cows late phase pregnancy their effects on udder health milk quality during first two weeks after calving. included 48 Holstein breed divided into four equal groups (n = 12). experimental group (D1) fed with addition total dose 1020 dl-a-tocopherol acetate Se/cow per day. second (D2) added at a 0.3 mg.kg-1 DM form sodium selenite. third (D3) supplemented combination selenite day 5.0 mg day, respectively....

In our study was followed occurrence of mastitis in herd 430 sheep breed zoslachtena valaska with hand milking technology examined two times during one lactation season. Individual examination consisted from clinical udder and microbiological milk samples. By PCR determined presence genes coding production enterotoxins, by ELISA methods individual types enterotoxins. From forms were frequently detected subacute (6.7%), subclinical (5.7%) acute (2.9%). The coagulase-negative staphylococci...

Acute phase proteins have been used as biomarkers of inflammation. Their concentrations increase in milk from cows with latent and subclinical mastitis. The aim our study was to evaluate amyloid A (MAA) indicator udder We 24 dairy a herd 120 Slovak Pied cattle. In addition bacteriological examination, the following indicators were determined all quarter samples. On basis results clinical Californian mastitis test (CMT), number Somatic cell count (SCC), four groups samples formed. levels MAA...

During the last three years (2015 to 2017), frequency of occurrence bacteria Staphylococcus spp. were examined in total 3466 individual and 12 pool milk samples. Experiment was carried out two herds breed sheep Improved Valaska region East Slovakia. Were isolated taxonomically identified 15 species genus (n = 444). From coagulase positive staphylococci (CPS), S. aureus during reporting period, however, most often first year (45). The incidence intermedius hyicus has been irregular. negative...

The occurrence of bacteria Staphylococcus spp. was examined in a total 3466 individuals and 12 pool milk samples from 2017 to 2019. experiment carried out two herds the breed sheep, Improved Valaska, Slovakia region. Eleven species genus (n = 431) were isolated taxonomically identified. From coagulase-positive staphylococci (CPS), S. aureus during reporting period, however, most often third year (50). incidence intermedius hyicus irregular. schleiferi highest at end follow-up duration....

Selenium and vitamin E ranks among very important antioxidant agents protecting the organism from effect of reactive oxygen forms. The deficiency both nutrients during pregnancy in cows often result metabolic disorders increased cases related diseases (mastitis, retained placenta other reproductive disorders). aim present work was to study influence different dose parenteral administration selenium dairy prior parturition on selected parameters, udder health milk quality. A total herd 270...

Marginal regions are relatively large part of the area Slovakia which in terms economy breeding ruminants can efficiently produce animal commodities only occasionally. Geographic, social and economic stability these is strongly influenced by with market milk production. Mastitis a disease complex that assumes highest clinical significance animals particularly medium to high yielding dairy cattle, usually around periparturient period. The objective this study was evaluate effectiveness...

Abstract The aim of this study was to evaluate the occurrence mastitis and its impact on reproductive parameters in a herd 180 dairy cows. Based records 127 cows Slovak spotted cattle their crosses with red Holstein were selected for between 1—2 months after calving. examination health status mammary glands consisted of: clinical udder, California test (CMT) supplemented by collection mixed milk samples, laboratory bacterial pathogens causing mastitis. In addition mam­mary investigation,...

The aim of the study was to evaluate electrical properties mammary gland dairy cow during manual and mechanical milking adapt electrographic method for analysis udder physiology. Udder myoelectrical activity measured on 10 healthy highly productive cows Slovak Pied cattle breed. four quarters recorded simultaneously using bipolar electrodes. stimulated by delicate two-finger massage, milking. During experiment duration reaction stimulation amplitude spike dischargers were listed. through...

Abstract Relatively large parts of the Slovak territory are marginal regions, which in terms economy ruminant rearing can effectively produce animal commodities only occasionally. The aim this study was to evaluate occurrence and aetiology mastitis during one milking season two sheep dairy herds 224 (A) 270 (B) ewes situated Slovakia. A complex examination health status based on: clinical udder, macroscopic evaluation milk with California test (CMT) bacteriological analysis raw samples from...

Abstract A relatively large part of the Slovak territory consists “marginal regions”, which in terms economy ruminants keeping can efficiently produce animal commodities only occasionally. The geographic, social and economic stability these regions is strongly influenced by rearing associated market milk production. aim this study was to evaluate prevalence aetiological agents mastitis two herds dairy cows situated marginal parts Slovakia. In total, 530 spotted breed Holstein were involved...

Mastitis is still one of the major causes economic losses in dairy sector. The routine application bacteriologic examination milk samples often insufficient and for this reason, alternative parameters are used to identify trends development udder health. Therefore, objectives study were determine relationship oxidative product levels, using malondialdehyde (MDA) as a marker on occurrence mastitis its causing pathogens. Dairy herd 223 Slovak spotted cattle tested etiology based assessment...