A5014764028- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Plant responses to elevated CO2
- Hepatitis C virus research
- Air Quality and Health Impacts
- Ferroptosis and cancer prognosis
- Urban Green Space and Health
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
Southern Medical University
2022-2025
Cell Technology (China)
2023-2025
Abstract Hepatocellular carcinoma (HCC) is a major cause of cancer‐related mortality, while the hepatocyte mechanisms driving oncogenesis remains poorly understood. In this study, single‐nucleus RNA sequencing samples from 22 HCC patients revealed 10 distinct subtypes, including beneficial Hep0, predominantly malignant Hep2, and immunosuppressive Hep9. These subtypes were strongly associated with patient prognosis, confirmed in TCGA‐LIHC Fudan cohorts through composition deconvolution. A...
DNA methylation is essential for a wide variety of biological processes, yet the development highly efficient and robust technology remains challenge routine single-cell analysis. We developed multiplex scalable reduced representation bisulfite sequencing (msRRBS) technology. It allows cell-specific barcoded fragments individual cells to be pooled before conversion, free enzymatic modification or physical capture ends, achieves read mapping rates 62.5 ± 3.9%, covering 60.0 1.4% CpG islands...
ABSTRACT DNA methylation is essential for a wide variety of biological processes, yet the development highly efficient and robust technology remains challenge routine single-cell analysis. We developed multiplex scalable reduced representation bisulfite sequencing (msRRBS) with off-the-shelf reagents equipment. It allows cell-specific barcoded fragments individual cells to be pooled before conversion, free enzymatic modification or physical capture ends, achieves unparalleled read mapping...