A5003361470- Animal Genetics and Reproduction
- Mesenchymal stem cell research
- Genetic and phenotypic traits in livestock
- Veterinary Oncology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Virus-based gene therapy research
- Genetic Mapping and Diversity in Plants and Animals
- Science and Education Research
- Tissue Engineering and Regenerative Medicine
- Chromosomal and Genetic Variations
- Periodontal Regeneration and Treatments
- Congenital Anomalies and Fetal Surgery
- Plant-Derived Bioactive Compounds
- Science and Science Education
- Sperm and Testicular Function
- Genetic diversity and population structure
- Urological Disorders and Treatments
- Mosquito-borne diseases and control
- Oral and Maxillofacial Pathology
- Fish biology, ecology, and behavior
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Sexual Differentiation and Disorders
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
Universidade Estadual Paulista (Unesp)
2013-2024
Universidade Federal Rural da Amazônia
2024
Amazon (United States)
2024
University of Guayaquil
2021
Universidade de São Paulo
2016
Reproductive Science Center
2012
National Institute of Environmental Health Sciences
2012
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2012
National Institutes of Health
2012
Center for Scientific Review
2012
Bone loss is a common problem after accidental traumas, cancers, congenital defects, and surgical procedures. The techniques normally used in large bone restoration involve complex invasive procedures such as grafting. Thus, it of interest to develop alternatives bioactive materials induce accelerated regeneration. Natural rubber (NR) membranes are potential candidates due their characteristics biocompatibility, angiogenic potential, flexibility, mechanical stability, surface porosity,...
ABSTRACT The aim of this study was to isolate, culture, and characterize mesenchymal stem cells (MSCs) from horse bone marrow (BM) using the techniques flow cytometry, immunocytochemistry, cytogenetics, electron microscopy. Immunophenotypic analysis revealed presence MSCs with high expression CD90 marker, lower CD44 absent CD34 marker. In assays differentiation, positive response osteogenic (OST), chondrogenic (CDG), adipogenic (ADP) differentiation signals observed characterized by...
Abstract Endometrial mesenchymal stem/progenitor cells (eMSCs) are multipotent known to modulate the immune system and have clinical application for human animal health. This makes these bovines attractive dual use as cellular therapy experimental model. The aim of this study was isolate, evaluate differentiation potential, immunophenotypic immunocytochemistry characteristics, chromosomal stability, cloning efficiency, cryopreservation response bovine eMSCs collected in two phases estrous...
Summary Objective: this study aimed to evaluate the LINE-1 transposon inserted in c-myc gene as a specific genetic alteration cells of spontaneous canine Transmissible Venereal Tumor (TVT) with either lymphocytoid or plasmacytoid phenotypes. Methods: tumoral biopsies from 35 dogs were collected by puncture exfoliation. Polymerase Chain Reaction (PCR) was carried out primers myc.s and LINE. A, segment detect presence LINE-1/c-myc molecular marker. Results: sequence alignment DNA samples TVT...
Cytotoxicity and subcutaneous tissue reaction of innovative blends composed by polyvinylidene fluoride fluoride-trifluoroethylene associated with natural polymers (natural rubber native starch) forming membranes were evaluated, aiming its applications bone regeneration. was evaluated in mouse fibroblasts culture cells (NIH3T3) using trypan blue staining. Tissue response vivo implantation materials rats, taking into account the presence necrosis connective capsule around implanted after 7,...
Abstract: Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples immunohistochemistry assays were taken from eight dogs cytological clinical diagnosis TVT. To validate immunochemical results TVT, a chromosome count was performed. For statistical analysis, Mann-Whitney test p<0.05 used. TVT tissues cells showed intense...
Due to numerous complications associated gastrointestinal augmented cystoplasty, this study aimed analyze the anatomic repair of bladder 10 female dogs using grafts porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them acellular SIS grafts. We assessed possible side effects each type graft by clinical examination, abdominal ultrasound laboratory findings. Anatomic neoformed was histological staining for H/E Masson's Trichrome, analyzed...
The aim of theis study was analyse different wild boars breeding in Sao Paulo state, entending to identify pure as well hybrid from mate with domestic swine.
Foram induzidas lesões no tendão flexor digital superficial (TFDS) de ambos os membros anteriores seis equinos, seguidas implante autólogo células da fração mononuclear medula óssea em apenas um membro cada animal. Os animais foram avaliados por parâmetros clínicos, ultrassonográficos, histopatológico e imunoistoquímico. Paralelamente, realizou-se o cultivo novas amostras para a caracterização das utilizando-se marcadores CD34 CD45 meio citometria fluxo, confirmando presença mesenquimais na...
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples, and implementation new in vitro studies. This work parts from the hypothesis that medium cryopreservation composed by 90% conditioned (CM) supports equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing maintenance biological properties establishment banks intended use Thus, we evaluated viability,...
Nine phenotypically normal Saanen goats (6 males and 3 females) belonging to the goat house of Instituto de Biociências, UNESP, Botucatu Campus, were analyzed cytogenetically. The objective present study was determine whether or not chromosomes 5 15 are involved in a Robertsonian translocation previously observed sample Brazilian herd using chromosome pattern described by ISCNDA (1989). results suggest involvement 6 fusion demonstrated G-banding prometaphase cells. animals carrying...
Ileocolonic aganglionosis (ICA) is the congenital and hereditary absence of neurons that constitute enteric nervous system has been described in various species including humans - Hirschsprung's disease horses overo lethal white syndrome (OLWS). affects circa 1 5,000 live births. At best, this means an inability to absorb nutrients from food (humans). worse, horses, it always death. Despite our general understanding functional mechanisms underlying ICA, there a paucity reliable quantitative...
Within about 30 years the Brazilian buffalo (Bubalus bubalis) herd will reach approximately 50 million head as a result of great adaptive capacity these animals to tropical climates, together with good productive and reproductive potential which make an important animal protein source for poor developing countries. The myostatin gene (GDF8) is in physiology stock because its product produces direct effect on muscle development consequently also meat production. sequence known several...
Babesiosis is one of the most important diseases affecting livestock agriculture worldwide. Animals from subspecies Bos taurus indicus are more resistant to babesiosis than those taurus. The genera Babesia and Plasmodium Apicomplexa hemoparasites share features such as invasion red blood cells (RBC). glycoprotein Duffy only human erythrocyte receptor for Pasmodium vivax a mutation which abolishes expression this on surfaces responsible making majority people originating indigenous...
The bush dog (Speothos venaticus) is a South American canid, included in the IBAMA (Brazilian Institute of Environment and Renewable Natural Resources) official list animals threatened with extinction, vulnerable category. As preservation conservation strategy, specimens kept captivity by Brazilian Institutions are monitored management plan. In order to characterize analyze genetic variability specimens, cytogenetic analysis was carried out, microsatellite data were also obtained through use...
Investigações sobre a sanidade e inter-relação homem-animal-meio ambiente possibilitam o registro de agentes etiológicos caráter zoonótico, como é caso dos arbovírus e, em relação aos primatas não-humanos (PNH), Amazônia se destaca por possuir uma rica diversidade desses animais que podem ser hospedeiros potencial para arbovírus. Nesse aspecto, trabalho objetivou avaliar ocorrência arboviroses PNH cativos no Estado do Pará. Para tanto, foram utilizadas amostras sangue 43 espécimes oriundos...