A5030997767- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Malaria Research and Control
- Insect and Pesticide Research
- Research on Leishmaniasis Studies
- Insect behavior and control techniques
- Neurobiology and Insect Physiology Research
- Studies on Chitinases and Chitosanases
- Insect Resistance and Genetics
- Study of Mite Species
- Trypanosoma species research and implications
- Fossil Insects in Amber
- Mycorrhizal Fungi and Plant Interactions
- Cocoa and Sweet Potato Agronomy
- Plant Virus Research Studies
- Aquaculture disease management and microbiota
Autonomous Healthcare
2022
Michigan State University
2019-2021
Fundação Oswaldo Cruz
2013
Kansas State University
2013
University of Glasgow
2003-2012
Johns Hopkins University
2008-2009
University of London
2003
London School of Hygiene & Tropical Medicine
2003
Abstract Background The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm the innate immune response against both bacteria and malaria parasites. To further characterize role hemocytes in immunity, we undertook first genome-wide transcriptomic analyses adult female An. following infection by two species parasite. Results We identified 4047 genes expressed hemocytes, using microarrays. While 279...
Abstract Wolbachia are intracellular, maternally transmitted bacteria considered the most abundant endosymbionts found in arthropods. They reproductively manipulate their host order to increase chances of being offspring, and currently used as a tool control vector-borne diseases. Studies on distribution among its arthropod hosts important both for better understanding why this bacterium is so common, well potential use biological agent. Here, we studied incidence broad range insect species,...
There is currently considerable interest and practical progress in using the endosymbiotic bacteria Wolbachia as a vector control agent for human vector-borne diseases. Such strategies may require introduction of multiple, different strains into target populations, necessitating identification characterization appropriate endosymbiont variants. Here, we report preliminary wFlu, native from neotropical mosquito Aedes fluviatilis, evaluate its potential by confirming ability to cause...
Wolbachia is a bacterial endosymbiont that naturally infects wide range of insect species, and causes drastic changes to host biology. Stable infections in mosquitoes can inhibit infection with medically important pathogens such as dengue virus malaria-causing Plasmodium parasites. However, some native strains enhance certain pathogens, the case for mosquito Aedes fluviatilis, where gallinaceum enhanced by wFlu strain. To better understand biological interactions between infections,...
Plasmodium falciparum gametocytes grown in vitro were fed through membrane feeders to laboratory-reared Anopheles stephensi mosquitoes. Intact midguts, including entire bloodmeal contents, removed between 24 and 48 h post-bloodfeeding. Giemsa-stained histological sections prepared from the midguts examined by light microscopy. Contrary previous reports, ookinetes clearly visible within midgut epithelial cells, demonstrating intracellular migration across wall. Ookinetes entered cells lateral...
Diversity in the surface antigens of malaria parasites is generally assumed to be a mechanism for immune evasion, but there little direct evidence that this leads evasion protective immunity. Here we show alleles highly polymorphic merozoite protein 2 (MSP-2) can grouped (within known dimorphic families) into distinct serogroups; variants within serogroup extensive serological cross-reactivity. Cross-reactive epitopes are immunodominant, and responses them may boosted at expense novel...
American cutaneous leishmaniasis (ACL) is a re-emerging disease in the state of São Paulo, Brazil. It important to understand both vector and distribution help design control strategies. As an initial step applying geographic information systems (GIS) remote sensing (RS) tools map disease-risk, objectives present work were to: (i) produce single database species distributions sand fly vectors (ii) create combined distributional maps incidence ACL its vectors, (iii) thereby provide individual...
Previous studies have shown that the central American mosquito vector, Anopheles albimanus, is generally refractory to oocyst infection with allopatric isolates of human malaria parasite Plasmodium falciparum. However, reasons for refractoriness A. albimanus such P. falciparum are unknown. In current study, we investigated infectivity clone 3D7A laboratory-reared and another natural vector malaria, stephensi. gametocytes grown in vitro were simultaneously fed both species progress compared....
Abstract The increasing availability of genome sequences and the development high‐throughput techniques for gene expression profiling functional characterization are transforming study innate immunity other areas insect biology. Already, genomic approaches have enabled a quantum advance in mosquito immune responses to malaria parasite infection, similar studies vector‐pathogen interactions can be expected near future. application microarray‐based analyses provide genome‐wide transcriptional...
Abstract Sandfly specimens from the subgenus Evandromyia ( Aldamyia ) Galati, 2003 (Diptera: Psychodidae: Phlebotominae) were collected between 2012 and 2019 nine localities in seven Brazilian states, morphologically‐identified, then DNA barcoded by sequencing mitochondrial cytochrome c oxidase subunit I coi gene. Forty‐four new barcode sequences generated 10 morphospecies combined with 49 previously published same analysed using sequence‐similarity methods (best‐match criteria) to assess...
There is currently considerable interest and practical progress in using the endosymbiotic bacteria Wolbachia as a vector control agent for human vector-borne diseases.Such strategies may require introduction of multiple, different strains into target populations, necessitating identification characterization appropriate endosymbiont variants.Here, we report preliminary wFlu, native from neotropical mosquito Aedes fluviatilis, evaluate its potential by confirming ability to cause cytoplasmic...
Bacteria associated with the parthenogenetic troglobiont sand fly Deanemyia maruaga were characterized by sequencing cloned 16S rDNA PCR products. Eleven novel partial sequences, varying degrees of similarity to Actinobacteria, identified. None sequences identified had homology those known from parthenogenesis-inducing bacteria.
To contribute to knowledge of the epidemiology American cutaneous leishmaniasis (ACL) among indigenous people living in sylvatic regions, we studied sand fly fauna collected areas disease transmission Brazilian Amazon. Here report two datasets comprising occurrence data for flies from Suruwaha Indigenous Land state Amazonas 2012–2013, and Wajãpi Amapá 2013–2014. Sand were using unbaited CDC-like light traps at various sites within each study area identified either genus or species-level by...