A5044506384- Microbial metabolism and enzyme function
- Methane Hydrates and Related Phenomena
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Biofuel production and bioconversion
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Climate change and permafrost
- Atmospheric and Environmental Gas Dynamics
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Protist diversity and phylogeny
- Carbon Dioxide Capture Technologies
- Fluid Dynamics and Heat Transfer
- Alcohol Consumption and Health Effects
- Advanced Research in Science and Engineering
- Plant Pathogens and Fungal Diseases
- Advanced Data Processing Techniques
- Fungal and yeast genetics research
- Peatlands and Wetlands Ecology
- Heme Oxygenase-1 and Carbon Monoxide
- Infectious Diseases and Mycology
- Geological Studies and Exploration
- Enzyme-mediated dye degradation
Russian Academy of Sciences
2013-2024
Czech Academy of Sciences, Institute of Microbiology
2023
Federal Center Research Fundamentals of Biotechnology
2021
University of Washington
2014-2015
The biotechnology of converting methane to single-cell protein (SCP) implies using fast-growing thermotolerant aerobic methanotrophic bacteria. Among the latter, members genus Methylococcus received significant research attention and are used in operating commercial plants. capsulatus MIR is a recently discovered member this with potential be for purpose SCP production. Like other species, bacterium stores carbon energy form glycogen, particularly when grown under nitrogen-limiting...
ABSTRACT The genomes of Methylosarcina lacus LW14 T (=ATCC BAA-1047 = JCM 13284 ), Methylobacter sp. strain 21/22, 31/32, Methylomonas LW13, MK1, and 11b were sequenced are reported here. All the strains obligately methanotrophic bacteria isolated from sediment Lake Washington.
Three strains of aerobic psychrotolerant methanotrophic bacteria Methylovulum psychrotolerans, isolated from geographically remote low-temperature environments in Northern Russia, were grown at three different growth temperatures, 20°C, 10°C and 4°C found to be capable oxidizing methane all temperatures. The M. psychrotolerans adapted their membranes decreasing temperature by increasing the percent unsaturated fatty acid (FAs), both for bulk intact polar lipid (IPL)-bound FAs. Furthermore,...
Two isolates of aerobic methanotrophic bacteria, strains Sph1T and Sph2, were obtained from cold methane seeps in a floodplain the river Mukhrinskaya, Irtysh basin, West Siberia. Another morphologically phenotypically similar methanotroph, strain OZ2, was isolated sediment subarctic freshwater lake, Archangelsk region, northern Russia. Cells these three Gram-stain-negative, light-pink-pigmented, non-motile, encapsulated, large cocci that contained an intracytoplasmic membrane system typical...
Rokubacteria is a phylogenetic clade of as-yet-uncultivated prokaryotes, which are detected in diverse terrestrial habitats and commonly addressed as members the rare biosphere. This was originally described candidate phylum; however, based on results comparative genome analysis, later defined order-level lineage, Rokubacteriales, within phylum Methylomirabilota. The physiology lifestyles these bacteria poorly understood. A dataset 16S rRNA gene reads retrieved from four boreal raised bogs...
ABSTRACT A complex system of muddy fluid-discharging and methane (CH 4 )-releasing seeps was discovered in a valley the river Mukhrinskaya, one small rivers Irtysh Basin, West Siberia. CH flux from most (90%) these gas ebullition sites did not exceed 1.45 g h −1 , while some emitted up to 5.54 . The δ 13 C value released varied between −71.1 −71.3‰, suggesting its biogenic origin. Although were characterized by low situ temperatures (3.5 5°C), relatively high rates oxidation (15.5 15.9 nmol...
The Methylocystis and Methylosinus are two of the five genera that were included in first taxonomic framework methanotrophic bacteria created half a century ago. Members both widely distributed various environments play key role reducing methane fluxes from soils wetlands. original separation these methanotrophs distinct was based mainly on their differences cell morphology. Further comparative studies explored single-gene-based phylogenies suggested monophyletic nature each genera. Current...
The bacterial genus Methylococcus , which comprises aerobic thermotolerant methanotrophic cocci, was described half-a-century ago. Over the years, a member of this genus, capsulatus Bath, has become major model organism to study genomic and metabolic basis obligate methanotrophy. High biotechnological potential fast-growing species, mainly as promising source feed protein, also been recognized. Despite big research attention, currently cultured diversity is represented by members two M....
Candidatus Methylospira mobilis is a recently described spiral-shaped, micro-aerobic methanotroph, which inhabits northern freshwater wetlands and sediments. Due to difficulties of cultivation, it could not be obtained in pure culture for long time. Here, we report on the successful isolation strain Shm1, first axenic this unique methanotroph. The complete genome sequence Shm1 was 4.7 Mb size contained over 4800 potential protein-coding genes. array genes encoding C1 metabolic capabilities...
Improvements in technology for the production of microbial biomass from natural gas, which have been gaining importance recent years, require development and optimization energy‐efficient bioreactor designs. The most advanced methods developing scaling up industrial biotechnological processes rely on detailed hydrodynamic modeling, addition to classical approaches, as main tool analysis large‐scale transfer experimental information. This paper presents results a simulation flows circuit...
Methylococcus capsulatus MIR is an aerobic methanotroph that was isolated from activated sludge sample and capable of growth on methanol. The finished genome strain 3.2 Mb in size. It encodes both MxaFI XoxF methanol dehydrogenases, as well three different isozymes formate dehydrogenase.
Methanotrophic bacteria that possess a unique ability of using methane as sole source carbon and energy have attracted considerable attention potential producers single-cell protein. So far, this biotechnology implied freshwater methanotrophs, although many regions the world limited resources. This study aimed at searching for novel methanotrophs capable fast growth in saltwater comparable composition with seawater. A methane-oxidizing microbial consortium containing Methylomarinum-...
An isolate of strictly aerobic, pale-pink pigmented bacteria, strain AF10T, was obtained from an organic soil layer in forested tundra, Nadym region, West Siberia. Cells AF10T were Gram-negative, non-motile rods that produced amorphous extracellular polysaccharide-like substance and formed large cell aggregates old cultures. These bacteria chemoorganotrophic, mildly acidophilic psychrotolerant, grew between pH 3.5 7.0 (optimum, 4.5-5.0) at temperatures 2 30 °C. The preferred growth...
Carotenoids are secondary metabolites that exhibit antioxidant properties and characterized by a striking range of colorations from red to yellow. These natural pigments synthesized wide eukaryotic prokaryotic organisms. Among the latter, carotenoid-producing methanotrophic bacteria, which display fast growth on methane or gas, particular interest as potential producers feed protein enriched with carotenoids. Until recently, Methylomonas strain 16a sp. ZR1 remained only representatives genus...
The family Gemmataceae accomodates aerobic, chemoorganotrophic planctomycetes with large genome sizes, is mostly distributed in freshwater and terrestrial environments. However, these bacteria have recently also been found locations relevant to human health. Since the antimicrobial resistance genes (AMR) from environmental resistome potential be transferred pathogens, it essential explore resistant capabilities of bacteria. In this study, reconstruction silico was performed for all nine...
The scavenging of atmospheric trace gases has been recognized as one the lifestyle-defining capabilities microorganisms in terrestrial polar ecosystems. Several metagenome-assembled genomes as-yet-uncultivated methanotrophic bacteria, which consume CH4 these ecosystems, have retrieved cultivation-independent studies. In this study, we isolated and characterized a representative methanotrophs, strain D3K7, from subarctic soil northern Russia. Strain D3K7 grows on methane methanol wide range...