A5012707488- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
Because of its dimensions, the recently introduced micropillar array columns are most suited for high-efficiency liquid chromatography separations in proteomics. Unlike packed bed and capillary-based column formats, concept still has significant room to progress terms reduction characteristic size (i.e., pillar diameter interpillar distance) open road even higher-efficiency their applications. We report here on on-chip comparison between first-generation (Gen 1) second-generation 2) wherein...
In the quest for better performing separation media liquid chromatography, micropillar array columns have received great interest over past years. While previous research was mainly focused around (μPACs) filled with cylindrical pillars, this contribution discusses μPACs rectangular which, first time, been anodized and hence carry a mesoporous shell. We report on series of on-chip measurements band broadening flow permeability in μPAC very wide radially elongated pillars (3·75 μm) an...
Abstract Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-state heterogeneity and distinct cellular signaling patterns that remain obscured with population-based techniques. The limited amount material encapsulated in a single cell however, raises significant technical challenges molecular profiling. Due extensive optimization efforts, mass spectrometry-based single-cell proteomics (scp-MS) has emerged as powerful tool facilitate proteome profiling...
In the field of liquid chromatography–mass spectrometry (LC–MS)-based proteomics, increases in sampling depth and proteome coverage have mainly been accomplished by rapid advances mass spectrometer technology. The comprehensiveness quality data that can be generated do, however, also depend on performance provided nano-liquid chromatography (nanoLC) separations. Proper selection reversed-phase separation columns important to provide MS instrument with peptides at highest possible...
ABSTRACT In the field of LC-MS based proteomics, increases in sampling depth and proteome coverage have mainly been accomplished by rapid advances mass spectrometer technology. The comprehensiveness quality data that can be generated do however also depend on performance provided nano liquid chromatography (nanoLC) separations. Proper selection reversed-phase separation columns important to provide MS instrument with peptides at highest possible concentration separated resolution. current...