A5064118902- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Virus-based gene therapy research
- Metabolomics and Mass Spectrometry Studies
- Cancer Genomics and Diagnostics
- Cell Image Analysis Techniques
Novo Nordisk Foundation
2022-2025
University of Copenhagen
2022-2025
Technical University of Munich
2022-2023
The Ohio State University
2022-2023
Technical University of Denmark
2022-2023
Thermo Fisher Scientific (Germany)
2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2023
Helmholtz Zentrum München
2021-2022
Max Planck Institute of Biochemistry
2022
Institute of Bioinformatics and Systems Biology
2021
Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-state heterogeneity and distinct cellular signaling patterns that remain obscured with population-based techniques. The limited amount material encapsulated in a single cell however, raises significant technical challenges molecular profiling. Due extensive optimization efforts, single-cell proteomics by Mass Spectrometry (scp-MS) has emerged as powerful tool facilitate proteome profiling from...
Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow named Chip-Tip, identifying >5,000 proteins HeLa It also facilitated direct detection post-translational modifications single cells, making need for specific modification-enrichment unnecessary. Our study demonstrates feasibility processing up 120 label-free samples per day. An optimized tissue dissociation...
Abstract Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated reverse transcription and integration of the de novo synthesized single-stranded DNA. To circumvent one main bottlenecks method, competition reverse-transcribed 3′ flap with original 5′ DNA, we generated an enhanced fluorescence-activated cell sorting reporter line to develop exonuclease-enhanced PE strategy (‘Exo-PE’) composed improved complex aptamer-recruited DNA-exonuclease remove DNA flap....
Abstract The emergence of mass spectrometry (MS)-based single-cell proteomics (SCP) promise to revolutionize the study cellular biology and biomedicine by providing an unparalleled view proteome in individual cells. Despite its groundbreaking potential, SCP is nascent faces challenges including limited sequence depth, throughput, reproducibility, which have constrained broader utility. This introduces key methodological advances, considerably improve sensitivity, coverage dependability...
A linear ion trap (LIT) is an affordable, robust mass spectrometer that provides fast scanning speed and high sensitivity, where its primary disadvantage inferior accuracy compared to more commonly used time-of-flight or orbitrap (OT) analyzers. Previous efforts utilize the LIT for low-input proteomics analysis still rely on either built-in OTs collecting precursor data OT-based library generation. Here, we demonstrate potential versatility of as a stand-alone analyzer all spectrometry (MS)...
In recent years, the concept of cell heterogeneity in biology has gained increasing attention, concomitant with a push toward technologies capable resolving such biological complexity at molecular level. For single-cell proteomics using Mass Spectrometry (scMS) and low-input experiments, sensitivity an orbitrap mass analyzer can sometimes be limiting. Therefore, scMS could benefit from linear ion traps, which provide faster scanning speeds higher than analyzer, however cost resolution. We...
Abstract Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-state heterogeneity and distinct cellular signaling patterns that remain obscured with population-based techniques. The limited amount material encapsulated in a single cell however, raises significant technical challenges molecular profiling. Due extensive optimization efforts, mass spectrometry-based single-cell proteomics (scp-MS) has emerged as powerful tool facilitate proteome profiling...
Expression of exon-specific isoforms from alternatively spliced mRNA is a fundamental mechanism that substantially expands the proteome cell. However, conventional methods to assess alternative splicing are either consumptive and work-intensive or do not quantify isoform expression longitudinally at protein level. Here, we therefore developed an reporter system (EXSISERS), which non-invasively reports translation exon-containing endogenous genes by scarlessly excising proteins nascent...
Despite their fundamental role in assessing (patho)physiological cell states, conventional gene reporters can follow expression but leave scars on the proteins or substantially alter mature messenger RNA. Multi-time-point measurements of non-coding RNAs are currently impossible without modifying nucleotide sequence, which native function, half-life and localization. Thus, we developed intron-encoded scarless programmable extranuclear cistronic transcript (INSPECT) as a minimally invasive...
ABSTRACT In recent years, the concept of cell heterogeneity in biology has gained increasing attention, concomitant with a push towards technologies capable resolving such biological complexity at molecular level. For single-cell proteomics using Mass Spectrometry (scMS) and low-input experiments, sensitivity an orbitrap mass analyzer can sometimes be limiting. Therefore, scMS could benefit from linear ion traps, which provide faster scanning speeds higher than analyzer, however cost...
A linear ion trap (LIT) is an affordable, robust mass spectrometer that proves fast scanning speed and high sensitivity, where its primary disadvantage inferior accuracy compared to more commonly used time-of-flight (TOF) or orbitrap (OT) analyzers. Previous efforts utilize the LIT for low-input proteomics analysis still rely on either built-in OTs collecting precursor data OT-based library generation. Here, we demonstrate potential versatility of as a stand-alone analyzer all spectrometry...