A5021699214- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Neuroblastoma Research and Treatments
- Alzheimer's disease research and treatments
- Metabolism, Diabetes, and Cancer
- Axon Guidance and Neuronal Signaling
- Peptidase Inhibition and Analysis
- Microtubule and mitosis dynamics
- Mass Spectrometry Techniques and Applications
- Protein Degradation and Inhibitors
- Cholinesterase and Neurodegenerative Diseases
- Cell death mechanisms and regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- Cancer Mechanisms and Therapy
- Melanoma and MAPK Pathways
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Hippo pathway signaling and YAP/TAZ
- Biosensors and Analytical Detection
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
Novo Nordisk Foundation
2015-2024
University of Copenhagen
1997-2024
A DNA-encoded small-molecule library was prepared using yoctoReactor technology followed by binder trap enrichment to identify selective inhibitors with nanomolar potencies against p38α MAP kinase.
Proteomic analysis of neurotrophin signaling identifies an inhibitory E3 ubiquitin ligase.
Tousled-like kinases (TLKs) are required for genome stability and normal development in numerous organisms have been implicated breast cancer intellectual disability. In humans, the similar TLK1 TLK2 interact with each other TLK activity enhances ASF1 histone binding is inhibited by DNA damage response, although molecular mechanisms of regulation remain unclear. Here we describe crystal structure kinase domain. We show that coiled-coil domains mediate dimerization essential activation...
An adaptor protein in the insulin signaling pathway enables survival of aggressive neuroblastoma cells.
Abstract Alzheimer’s disease (AD) is a progressive and irreversible neurodegenerative characterized by the accumulation of aggregated amyloid beta (Aβ) hyperphosphorylated tau along with slow decline in cognitive functions. Unlike advanced AD, initial steps AD pathophysiology have been poorly investigated, partially due to limited availability animal models focused on early, plaque-free stages disease. The aim this study was evaluate early behavioral, anatomical molecular alterations...
A linear ion trap (LIT) is an affordable, robust mass spectrometer that provides fast scanning speed and high sensitivity, where its primary disadvantage inferior accuracy compared to more commonly used time-of-flight or orbitrap (OT) analyzers. Previous efforts utilize the LIT for low-input proteomics analysis still rely on either built-in OTs collecting precursor data OT-based library generation. Here, we demonstrate potential versatility of as a stand-alone analyzer all spectrometry (MS)...
Synovial fibroblasts (SFs) are key pathogenic drivers in rheumatoid arthritis (RA). Their vivo activation by TNF is sufficient to orchestrate full arthritic pathogenesis animal models, and blockade proved efficacious for a high percentage of patients with RA albeit coinducing rare but serious side effects. Aiming find new potent therapeutics, we applied the L1000CDS2 search engine, repurpose drugs that could reverse expression signature arthritogenic human TNF-transgenic (hTNFtg) SFs. We...
Neuroblastoma is a highly heterogeneous embryonal solid tumor of the sympathetic nervous system. As some tumors can be treated to undergo differentiation, investigating this process guide differentiation-based therapies neuroblastoma. Here, we studied role E3 ubiquitin ligases Cbl and Cbl-b in regulation long-term signaling responses associated with extracellular signal-regulated kinase phosphorylation neurite outgrowth, morphological marker neuroblastoma cell differentiation. Using...
The human MASTL (Microtubule-associated serine/threonine kinase-like) gene encodes an essential protein in the cell cycle. is a key factor preventing early dephosphorylation of M-phase targets Cdk1/CycB. Little known about mechanism activation and regulation. contains non-conserved insertion 550 residues within its loop, splitting kinase domain, making it unique. Here, we show that this middle region (NCMR) crucial for target specificity activity. We performed phosphoproteomic assay with...
A linear ion trap (LIT) is an affordable, robust mass spectrometer that proves fast scanning speed and high sensitivity, where its primary disadvantage inferior accuracy compared to more commonly used time-of-flight (TOF) or orbitrap (OT) analyzers. Previous efforts utilize the LIT for low-input proteomics analysis still rely on either built-in OTs collecting precursor data OT-based library generation. Here, we demonstrate potential versatility of as a stand-alone analyzer all spectrometry...
Insulin resistance impairs the cellular insulin response, and often precedes metabolic disorders, like type 2 diabetes, impacting an increasing number of people globally. Understanding molecular mechanisms in hepatic is essential for early preventive treatments. To elucidate changes signal transduction associated with hepatocellular resistance, we employed a multi-layered mass spectrometry-based proteomics approach focused on receptor (IR) signaling at interactome, phosphoproteome, proteome...
Abstract Insulin resistance impairs the cellular insulin response and frequently precedes metabolic disorders, like type 2 diabetes, which are affecting an increasing number of people globally. Given critical role liver in glucose lipid metabolism, understanding molecular mechanisms hepatic is essential for early preventive treatments. To elucidate changes signal transduction associated with hepatocellular resistance, we employed a multi-layered mass spectrometry-based proteomics approach...