A5058816989- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Neuroscience and Neural Engineering
- Viral Infections and Immunology Research
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Receptor Mechanisms and Signaling
- Connexins and lens biology
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Neuropeptides and Animal Physiology
- Insect and Arachnid Ecology and Behavior
- Biocrusts and Microbial Ecology
- Advanced biosensing and bioanalysis techniques
- Adenosine and Purinergic Signaling
- bioluminescence and chemiluminescence research
Center for Environmental Health
2024
Helmholtz Zentrum München
2015-2024
Institute of Bioinformatics and Systems Biology
2021-2024
Technical University of Munich
2014-2024
Institute of Biological and Medical Imaging
2020
Center for Integrated Protein Science Munich
2014
Using CRISPR/Cas9, it is possible to target virtually any gene in organism. A major limitation its application therapy the size of Cas9 (>4 kb), impeding efficient delivery via recombinant adeno-associated virus (rAAV). Therefore, we developed a split–Cas9 system, bypassing packaging limit using split-inteins. Each half was fused corresponding split-intein moiety and, only upon co-expression, intein-mediated trans-splicing occurs and full protein reconstituted. We demonstrated that nuclease...
Abstract Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated reverse transcription and integration of the de novo synthesized single-stranded DNA. To circumvent one main bottlenecks method, competition reverse-transcribed 3′ flap with original 5′ DNA, we generated an enhanced fluorescence-activated cell sorting reporter line to develop exonuclease-enhanced PE strategy (‘Exo-PE’) composed improved complex aptamer-recruited DNA-exonuclease remove DNA flap....
Acute stimulation of cardiac β-adrenoceptors is crucial to increasing function under stress; however, sustained β-adrenergic has been implicated in pathological myocardial remodeling and heart failure. Here, we have demonstrated that export cAMP from myocytes an intrinsic cardioprotective mechanism response stress. We report infusion into mice averted hypertrophy fibrosis a disease model pressure overload. The protective effect exogenous required adenosine receptor signaling. This...
Engineering functional 3D tissue constructs is essential for developing advanced organlike systems, with applications ranging from fundamental biological research to drug testing. The generation of complex multicellular structures requires the integration external geometric and mechanical cues ability activate genetic programs that regulate stimulate cellular self-organization. Here, we demonstrate gelatin methacryloyl (GelMA) hydrogels serve as effective matrices cell culture, supporting...
Abstract Background Many long noncoding RNAs (lncRNAs) have been implicated in general and cell type-specific molecular regulation. Here, we asked what underlies the fundamental basis for seemingly random appearance of nuclear lncRNA condensates cells, sought compounds that can promote disintegration vivo. Results As a comparing lncRNAs cellular properties among different types, screened human pluripotent stem cells (hPSCs) were differentiated to an atlas lineages. We found paraspeckles,...
Expression of exon-specific isoforms from alternatively spliced mRNA is a fundamental mechanism that substantially expands the proteome cell. However, conventional methods to assess alternative splicing are either consumptive and work-intensive or do not quantify isoform expression longitudinally at protein level. Here, we therefore developed an reporter system (EXSISERS), which non-invasively reports translation exon-containing endogenous genes by scarlessly excising proteins nascent...
<title>Abstract</title> Evolution guides biological systems to populate ecological niches, with viruses being one of the most successful examples that principle. Viruses evolved over billions years for efficient transfer nucleic acids. Although highly diverse, converged toward a remarkable similarity in size and shape their capsids. In contrast, generative models protein design enable creation architectures are absent nature. Here, we investigate whether AI-designed assemblies can be...
Heterologous enzymes and binding proteins were secreted by the moss Physcomitrella patens or anchored extracellularly on its cell membrane in order to functionalize apoplast as a biochemical reaction compartment. This modular anchoring system utilizes signal peptide transmembrane segment of somatic embryogenesis receptor-like kinase (SERK), which identified comprehensive bioinformatic analysis P. genome. By fusing soluble enzyme NanoLuc luciferase peptide, secretion capability was confirmed...
Despite their fundamental role in assessing (patho)physiological cell states, conventional gene reporters can follow expression but leave scars on the proteins or substantially alter mature messenger RNA. Multi-time-point measurements of non-coding RNAs are currently impossible without modifying nucleotide sequence, which native function, half-life and localization. Thus, we developed intron-encoded scarless programmable extranuclear cistronic transcript (INSPECT) as a minimally invasive...
Abstract Despite their pivotal role in evaluating (patho)physiological cell states, traditional gene reporters can follow expression but leave marks on proteins or significantly modify mature mRNA. Multi-time point readouts of non-coding RNAs are, to date, not applicable without changing nucleotide sequence, which may affect natural function, half-life, and localization. We thus created INSPECT (Intron-encoded Scarless Programmable Extranuclear Cistronic Transcripts) as a minimally invasive...
Background Studies aiming at the targeted activation of tumor-suppressive p53 isoforms have revealed promising and novel therapeutic approaches. Besides pro-apoptotic full-length (FL)p53 Δ40p53, Δ133p53 Δ160p53 act as negative regulators inducing anti-apoptotic effects. We established a exon-specific luciferase-based reporter system (EXISERS) that allows for multiplexed quantification differentiation target-protein in real-time living cells. Its high-throughput capability systematic...
Transcriptomics is a valuable technique for multiplexed monitoring of cellular states. However, its application has been predominantly limited to assays that necessitate cell fixation or lysis, which hinders real-time, longitudinal tracing RNA expression in live cells. Here, we present non-destructive transcriptomics by vesicular export (NTVE) enable multi-time-point dynamics living inducible reporter lines. Stabilized barcodes can be selectively packaged and exported from cells via...
Abstract Exon-specific isoform expression from alternatively spliced mRNA is a fundamental mechanism that substantially enriches cell’s proteome. However, conventional methods to assess alternative splicing are either consumptive and work-intensive or do not quantify over multiple time points at the protein level. We thus developed an exon-specific reporter system (EXSISERS), which non-invasively reports translation of exon-containing isoforms by scarlessly excising proteins nascent...