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A5093842311
Research Areas
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
Abstract Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated reverse transcription and integration of the de novo synthesized single-stranded DNA. To circumvent one main bottlenecks method, competition reverse-transcribed 3′ flap with original 5′ DNA, we generated an enhanced fluorescence-activated cell sorting reporter line to develop exonuclease-enhanced PE strategy (‘Exo-PE’) composed improved complex aptamer-recruited DNA-exonuclease remove DNA flap....
10.1038/s41592-023-02162-w
article
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Nature Methods
2024-02-01
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