A5102825842- Solidification and crystal growth phenomena
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- High Temperature Alloys and Creep
- Chemical Synthesis and Analysis
- nanoparticles nucleation surface interactions
- Aluminum Alloy Microstructure Properties
- Amino Acid Enzymes and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Advanced Nanomaterials in Catalysis
- Crystallization and Solubility Studies
- GABA and Rice Research
- Advanced Photocatalysis Techniques
- Carbohydrate Chemistry and Synthesis
- Metallurgy and Material Forming
- Electrochemical sensors and biosensors
- Biochemical and Molecular Research
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
East China University of Science and Technology
2020-2023
Shandong Academy of Sciences
2021
Qilu University of Technology
2021
Czech Academy of Sciences, Institute of Biotechnology
2020
Chinese Academy of Sciences
2012
Abstract ω‐Transaminases (ω‐TAs) show considerable potential for the synthesis of chiral amines. However, their low catalytic efficiency towards bulky substrates limits application, and complicated mechanisms prevent precise enzyme design. Herein, we address this challenge using a mechanism‐guided computational design strategy by reprograming transition ground states in key reaction steps. The common features among three high‐energy‐barrier steps responsible were revealed quantum mechanics...
Abstract The asymmetric synthesis of chiral amino acids by leucine dehydrogenases has great potential for industrialization; however, the inhibitory effect high‐concentration substrates limits its large‐scale application. Herein, using structure‐guided genome mining based on sequence‐structure prediction substrate tolerance and specificity, a novel dehydrogenase ( La LeuDH) from Labrenzia aggregate was identified characterized, which exhibited highest excellent activity to trimethyl...
L-2-aminobutyric acid (L-2-ABA) is a chiral precursor for the synthesis of anti-epileptic drug levetiracetam and anti-tuberculosis ethambutol. Asymmetric L-2-ABA by leucine dehydrogenases has been widely developed. However, limitations natural enzymes, such as poor stability, low catalytic efficiency, inhibition high-concentration substrates, limit large-scale applications. Herein, directed screening metagenomic library from unnatural amino acid-enriched environments, robust dehydrogenase,...
Enzymatic asymmetric synthesis of chiral amino acids has great industrial potential. However, the low catalytic efficiency high-concentration substrates limits their application. Herein, using a combination substrate prediction based on "open to closed" conformational change and specificity prediction, novel leucine dehydrogenase (TsLeuDH), with high toward benzoylformic acid (BFA) for producing l-phenylglycine (l-Phg), was directly identified from 4695 putative dehydrogenases in public...
CdS net framework (CdS-NF) nanoparticles were synthesized under mild reaction conditions and used to construct an <italic>Escherichia coli</italic>–CdS-NF hybrid system which NADH regeneration promote the redox reaction.
Abstract ω‐Transaminases (ω‐TAs) show considerable potential for the synthesis of chiral amines. However, their low catalytic efficiency towards bulky substrates limits application, and complicated mechanisms prevent precise enzyme design. Herein, we address this challenge using a mechanism‐guided computational design strategy by reprograming transition ground states in key reaction steps. The common features among three high‐energy‐barrier steps responsible were revealed quantum mechanics...