A5007689920- Enzyme Catalysis and Immobilization
- Nuclear physics research studies
- Astronomical and nuclear sciences
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Acoustic Wave Resonator Technologies
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Nuclear Physics and Applications
- Quantum Chromodynamics and Particle Interactions
- Atomic and Molecular Physics
- HVDC Systems and Fault Protection
- Chemical Synthesis and Analysis
- Biofuel production and bioconversion
- Ecology and Conservation Studies
- Plant Parasitism and Resistance
- Allelopathy and phytotoxic interactions
- Biochemical and Molecular Research
- Pancreatic function and diabetes
- Protein Hydrolysis and Bioactive Peptides
- Innovative Microfluidic and Catalytic Techniques Innovation
- High-pressure geophysics and materials
- High-Voltage Power Transmission Systems
- Advanced Chemical Physics Studies
East China University of Science and Technology
2015-2025
Guizhou University
2008-2024
Zhengzhou University
2011-2024
China National Research Institute of Food and Fermentation
2024
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences
2022-2023
University of Electronic Science and Technology of China
2008-2023
Changsha University of Science and Technology
2021-2022
Czech Academy of Sciences, Institute of Biotechnology
2017-2021
State Council of the People's Republic of China
2020-2021
Xidian University
2018
Marine mud is an abundant and largely unexplored source of enzymes with unique properties that may be useful for industrial biotechnological purposes. However, since most microbes cannot cultured in the laboratory, a cultivation-independent metagenomic approach would advantageous identification novel enzymes. Therefore, objective screening lipolytic enzymes, library was constructed using total genomic DNA extracted from marine mud. Based on functional heterologous expression, 34 clones...
α-Amylase plays a pivotal role in broad range of industrial processes. To meet increasing demands biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. However, the relevant data α-amylases from cold-adapted fungi are still insufficient. In addition, bread quality presents particular interest due its high consummation. Thus developing amylases improve textural properties could combine health benefits with good sensory properties. Furthermore,...
Abstract BACKGROUND In our previous study, we successfully identified five peptides from wheat gluten: Ala‐Pro‐Ser‐Tyr (APSY), Leu‐Tyr (LY), Pro‐Tyr (PY), Arg‐Gly‐Gly‐Tyr (RGGY) and Tyr‐Gln (YQ). Molecular docking molecular dynamics simulation methods were employed to investigate the interaction between these antioxidant Kelch‐like ECH‐associated protein 1 (Keap1 protein), revealing mechanism of their non‐competitive binding. addition, total capacity was determined using...
Enzymatic asymmetric synthesis of l-phenylglycine by amino acid dehydrogenases has potential for industrial applications; however, this is hindered their low catalytic efficiency toward high-concentration substrates. We identified and characterized a novel leucine dehydrogenase (MsLeuDH) with high benzoylformic via directed metagenomic approaches. Further, we obtained triple-point mutant MsLeuDH-EER (D332E/G333E/L334R) improved stability through the rational design distal loop 13. A...
Abstract Background A nitrilase-mediated pathway has significant advantages in the production of optically pure (R)-(−)-mandelic acid. However, unwanted byproduct, low enantioselectivity, and specific activity reduce its value practical applications. An ideal nitrilase that can efficiently hydrolyze mandelonitrile to acid without byproduct is needed. Results novel (BCJ2315) was discovered from Burkholderia cenocepacia J2315 through phylogeny-based enzymatic substrate specificity prediction...
Abstract ω‐Transaminases (ω‐TAs) show considerable potential for the synthesis of chiral amines. However, their low catalytic efficiency towards bulky substrates limits application, and complicated mechanisms prevent precise enzyme design. Herein, we address this challenge using a mechanism‐guided computational design strategy by reprograming transition ground states in key reaction steps. The common features among three high‐energy‐barrier steps responsible were revealed quantum mechanics...
(R)-(−)-Mandelic acid is an important chiral building block that widely used in pharmacy and the production of fine chemicals. A more advanced method for obtaining (R)-(−)-mandelic direct hydrolysis corresponding racemic mandelonitrile. In order to develop a cost-effective process, highly efficient enantioselective nitrilase BCJ2315 from Burkholderia cenocepacia J2315 was biotransformation mandelonitrile acid. The recombinant Escherichia coli M15/BCJ2315 showed high substrate tolerance could...
High-accuracy mass measurements of neutron-deficient Yb isotopes have been performed at TRIUMF using TITAN's multiple-reflection time-of-flight spectrometer (MR-TOF-MS). For the first time, an MR-TOF-MS was used on line simultaneously as isobar separator and a spectrometer, extending to two further away from stability than otherwise possible. The ground state masses $^{150,153}\mathrm{Yb}$ excitation energy $^{151}{\mathrm{Yb}}^{m}$ were measured for time. As result, persistence $N=82$ shell...
The magnetic chitosan nanocomposites have been studied intensively and used practically in various biomedical biological applications including enzyme immobilization. However, the loading capacity remained activity of immobilized based on existing approaches are not satisfied. Simpler more effective immobilization strategies needed. Here we report a simple catechol modified protocol for preparing novel catechol-chitosan (CCS) - iron oxide nanoparticles (IONPs) composites carrying adhesive...
Quadrupole and octupole stiffnesses in the ground states of even-even $^{112\ensuremath{-}150}\mathrm{Ba}$ isotopes have been systematically investigated by means potential-energy-surface calculations. The calculations are carried out both $({\ensuremath{\beta}}_{2},\ensuremath{\gamma},{\ensuremath{\beta}}_{4})$...
Bioreductions catalyzed by alcohol dehydrogenases (ADHs) play an important role in the synthesis of chiral alcohols. However, ethyl (S)-4-chloro-3-hydroxybutyrate [(S)-CHBE], drug intermediate, has significant challenges concerning high substrate or product inhibition toward ADHs, which complicates its production. Herein, we evaluated a novel ADH, SmADH31, obtained from Stenotrophomonas maltophilia genome, can tolerate extremely concentrations (6 M) both and product. The coexpression SmADH31...
This study aimed to gain an understanding of the possible function NACs by examining their physicochemical properties, structure, chromosomal location, and expression. Being a family plant-specific transcription factors, NAC (petunia no apical meristem Arabidopsis thaliana ATAF1, ATAF2, CUC2) is involved in plant growth development. None genes has been reported Akebia trifoliata (Thunb.) Koidz (A. trifoliata). In this study, we identified 101 proteins (AktNACs) A. genome bioinformatic...
The young shoots of the tea plant Baiye No. 1 display an albino phenotype in early spring under low environmental temperatures, and leaves re-green like those common cultivars during warm season. Periodic albinism is precisely regulated by a complex gene network that leads to metabolic differences enhances nutritional value leaves. Here, we identified messenger RNAs (mRNAs), long noncoding (lncRNAs), circular (circRNAs), microRNAs (miRNAs) construct competing endogenous RNA (ceRNA)...
The nitrilase-mediated pathway has significant advantages in the production of optically pure aromatic α-hydroxy carboxylic acids. However, low enantioselectivity and activity are observed on hydrolyzing o-chloromandelonitrile to produce (R)-o-chloromandelic acid. In present study, a protein engineering approach was successfully used enhance performance nitrilase obtained from Burkholderia cenocepacia strain J2315 (BCJ2315) o-chloromandelonitrile. Four hot spots (T49, I113, Y199, T310)...
Abstract In order to explore the structures and combination mechanism of wheat gluten peptides-calcium chelate (WOP-Ca) in depth, WOP-Ca were prepared by chelating peptides (WOP) with calcium. The yield was determined be 51.2 ± 2.12 %, it exhibited a calcium-chelating rate 58.96 1.38 %. structural differences between WOP characterized using various analytical techniques, results revealed that differed their microstructure, characteristic group absorption peaks, changes electron cloud...
Elicitation is a well-known strategy for increasing the accumulation of secondary metabolites in callus cultures. In this study, induction system Saxifraga stolonifera Meerb. was developed, and elicitors were used to enhance metabolite production. Tender leaves S. as explants vitro culture. We examined characteristics its response various by assessing sterilization conditions explants, varying hormone ratios, determining optimal concentrations salicylic acid (SA), Sodium nitroprusside (SNP)...
A solvent engineering approach and an extended fed-batch reaction mode were introduced to increase the activity enantioselectivity alleviate substrate inhibition of nitrilase BCJ2315 from Burkholderia cenocepacia J2315 toward o-chloromandelonitrile. Among seven water-miscible organic solvents tested, ethanol (30%, v/v) demonstrated highest conversion (55.7%) (enantiomeric excess, 98.2% ee) compared with those control [which did not contain any (13% 89.2%, respectively)] was thus chosen as...
Static fission barriers for 95 even-even transuranium nuclei with charge number Z = 94–118 have been systematically investigated by means of pairing self-consistent Woods-Saxon-Strutinsky calculations using the potential energy surface approach in multidimensional (β2, γ, β4) deformation space. Taking heavier 252Cf nucleus (with available barrier from experiment) as an example, formation and influence macroscopic, shell correction energies on it are analyzed. The results present calculated...