A5032063058- Pancreatic function and diabetes
- Connexins and lens biology
- Metabolism, Diabetes, and Cancer
- Adenosine and Purinergic Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Diabetes and associated disorders
- Cellular Mechanics and Interactions
- Regulation of Appetite and Obesity
- Erythrocyte Function and Pathophysiology
- Chronic Kidney Disease and Diabetes
- Protein Kinase Regulation and GTPase Signaling
- Advanced Glycation End Products research
- Diet, Metabolism, and Disease
- Force Microscopy Techniques and Applications
- Receptor Mechanisms and Signaling
- Calcium signaling and nucleotide metabolism
- Neuroscience of respiration and sleep
- TGF-β signaling in diseases
- Microfluidic and Bio-sensing Technologies
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- Cardiovascular Effects of Exercise
- Wnt/β-catenin signaling in development and cancer
University of Lincoln
2014-2025
Manchester City Football Club
2020-2022
Lincoln University - Pennsylvania
2022
University of Warwick
2005-2014
Coventry (United Kingdom)
2012
Biomedical Research Institute
2006-2008
King's College London
1999-2002
Guy's Hospital
2000
University of British Columbia
1996-1999
University of Sheffield
1992-1997
The effect of cell-to-cell contact on Ca2+ influx and secretory responses in the beta-cell line MIN6 was studied using pseudoislets, which are three-dimensional islet-like cell aggregates that develop when cells cultured for 6-8 days gelatin. formation pseudoislets is dependent Ca2+-dependent adhesion molecule E-cadherin (E-CAD), since process can be inhibited by incubation absence or presence an anti-E-CAD antibody. Glucose alpha-ketoisocaproic acid (KIC) evoked a only small fraction...
Abstract: Melatonin is known to inhibit insulin secretion from rodent β ‐cells through interactions with cell‐surface MT 1 and/or 2 receptors, but the function of this hormone in human islets Langerhans not known. In current study, melatonin receptor expression by was examined reverse transcription‐polymerase chain reaction (RT‐PCR) and effects exogenous on intracellular calcium ([Ca 2+ ] i ) levels islet were determined single cell microfluorimetry radioimmunoassay, respectively. RT‐PCR...
The presence of the extracellular calcium-sensing receptor on human antral gastrin cells was investigated. Reverse transcription PCR using mRNA isolated from cell- enriched cell cultures identified a product with sequence identical to part parathyroid-secreting receptor. Immunocytochemistry an antibody region immunostained all (but not mucin or somatostatin cells), and detected appropriate-sized bands in Western blots whole lysates. Increasing calcium levels 0.5 9 mM stimulated release...
Background/Aims: The efficient secretion of insulin from β-cells requires extensive intra-islet communication. cell surface adhesion protein epithelial (E)-cadherin (ECAD) establishes and maintains tissues such as the islets Langerhans. In this study, role ECAD in regulating pseudoislets was investigated. Methods: effect an immuno-neutralising on gross morphology, cytosolic calcium signalling, direct cell-to-cell communication assessed by fura-2 microfluorimetry, Lucifer Yellow dye injection...
Diabetes mellitus remains a prevalent condition worldwide and significant risk factor for atherosclerotic cardiovascular disease. Recent evidence suggests the use of glucose-lowering therapies with benefit in optimising cardiometabolic profile patients type 2 diabetes mellitus. However, uptake low. This study was carried out to assess impact novel cardiodiabetes service management presenting acute coronary syndromes. A retrospective, observational, registry-based analysis performed among an...
The presence and functional significance of the extracellular calcium-sensing receptor (CaR) on human pancreatic beta-cells were investigated. Reverse transcriptase-polymerase chain reaction with primers for domain CaR expressed in parathyroid-secreting cells identified a product expected size mRNA. Immunocytochemistry using an antibody against region showed extensive immunoreactivity insulin- glucagon-containing but not somatostatin-containing cells. In perifusion experiments, elevations...
A preterm female infant presented with intractable hypoglycaemia within 10 minutes of delivery. Normoglycaemia could be maintained only by the intravenous infusion glucose at a rate 20-22 mg/kg/min. Persistent hyperinsulinaemic infancy was diagnosed from an inappropriately raised plasma insulin concentration (33 mU/l) time (blood < 0.5 mmol/l). Medical treatment glucagon, somatostatin, and diazoxide led to modest reduction in requirement; 95% pancreatectomy performed histological...
The extracellular calcium-sensing receptor (CaR) is usually associated with systemic Ca 2+ homeostasis, but the CaR also expressed in many other tissues, including pancreatic islets of Langerhans. In present study, we have used human and an insulin-secreting cell line (MIN6) to investigate effects activation using calcimimetic R-568, a agonist that activates at physiological concentrations . initiated marked transient insulin secretory response from both MIN6 cells sub-stimulatory...
The effect of short-chain fatty acids (SCFAs) on the contractile activity and fluid output large bowel rat was studied using an isolated segment cecum colon, mounted in vitro. rate per minute proximal, mid, distal regions colon depressed by luminal infusion associated SCFAs either as a mixture (acetic, propionic, butyric) or individually (100 mM/pH = 4.1, each case). Dose responses were observed for individual acids, with 100 mM solutions eliciting more prominent reduction colonic motor than...
Background/Aims: Changes in cell-to-cell communication have been linked to several secondary complications of diabetes, but the mechanism by which connexins affect disease progression kidney is poorly understood. This study examines a role for glucose-evoked changes beta1 isoform transforming growth factor (TGFβ1), on connexin expression, gap-junction mediated intercellular (GJIC) and hemi-channel ATP release from tubular epithelial cells proximal renal nephron. Methods: Biopsy material...
Tubulointerstitial fibrosis represents the key underlying pathology of Chronic Kidney Disease (CKD), yet treatment options remain limited. In this study, we investigated role connexin43 (Cx43) hemichannel-mediated adenosine triphosphate (ATP) release in purinergic-mediated disassembly adherens and tight junction complexes early tubular injury.Human primary proximal tubule epithelial cells (hPTECs) clonal (HK2) were treated with Transforming Growth Factor Beta1 (TGF-β1) ± apyrase, or ATPγS...
Without a viable cure, chronic kidney disease is global health concern. Inflammatory damage in and around the renal tubules dictates severity contributed to by multiple cell types. Activated response danger associated molecular patterns (DAMPs) including ATP, NOD-like receptor protein-3 (NLRP3) inflammasome integral this inflammation. In vivo, we have previously observed that increased expression of Connexin 43 (Cx43) linked inflammation (CKD) whilst vitro studies human proximal tubule cells...
Background/Aim: Diabetic nephropathy is associated with progressive renal damage, leading to impaired function and end-stage failure. Secondary hypertension stems from a deranged ability of cells within the kidney resolve appropriately regulate sodium resorption in response hyperglycaemia. However, mechanisms by which glucose alters re-uptake have not been fully characterised. Methods: Here we present RT-PCR, western blot immunocytochemistry data confirming mRNA protein expression serum...