A5067267335- Asthma and respiratory diseases
- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Receptor Mechanisms and Signaling
- Urticaria and Related Conditions
- Pulmonary Hypertension Research and Treatments
- Supramolecular Self-Assembly in Materials
- Sinusitis and nasal conditions
- Phytochemical compounds biological activities
- Sesquiterpenes and Asteraceae Studies
- Eosinophilic Disorders and Syndromes
- Respiratory and Cough-Related Research
- Mesenchymal stem cell research
- Monoclonal and Polyclonal Antibodies Research
- Natural product bioactivities and synthesis
- Adenosine and Purinergic Signaling
- Wound Healing and Treatments
- Silk-based biomaterials and applications
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Peroxisome Proliferator-Activated Receptors
- Insects and Parasite Interactions
National Institute for Nanotechnology
2016-2023
National Research Council Canada
2016-2023
University of Alberta
2011-2021
Mexican Social Security Institute
2001
We have shown that proteinase-activated receptor-2 (PAR(2)) activation in the airways leads to allergic sensitization concomitantly inhaled Ags, thus implicating PAR(2) pathogenesis of asthma. Many aeroallergens with proteinase activity activate PAR(2). To study role aeroallergens, we developed a murine model mucosal cockroach proteins. hypothesized by natural allergens serine plays an important sensitization. Cockroach extract (CE) was administered BALB/c mice intranasally on five...
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Summary Background Proteinase‐activated receptor 2 ( PAR ) is a G protein‐coupled activated by trypsin‐like serine proteinases. activation has been associated with inflammation including allergic airway inflammation. We have also shown that in the airways leads to sensitization. The exact contribution of development eosinophilic and hyperresponsiveness AHR sensitized individuals not clear. Objective To investigate whether functional inhibition during allergen challenge mice would inhibit...
Background Many aeroallergens contain proteinase activity and are able to induce allergic sensitization when presented mucosal surfaces. Some of these allergens activate proteinase-activated receptor-2 (PAR2). Objective To determine the role PAR2 activation in a murine house dust mite (HDM) allergy model. Methods We sensitized challenged PAR2-deficient mice with HDM, examined outcomes compared wild-type animals. focus on sensitization, we administered blocking antibody animals during phase...
Pulmonary fibrotic diseases induce significant morbidity and mortality, for which there are limited therapeutic options available. Rac2, a ras-related guanosine triphosphatase expressed mainly in hematopoietic cells, is crucial molecule regulating diversity of mast cell, macrophage, neutrophil functions. All these cell types have been implicated the development pulmonary fibrosis variety animal models. For studies described here we hypothesized that Rac2 deficiency protects mice from...
Mast cells play an important role in disease pathogenesis by secreting immunomodulatory molecules. are primarily activated the crosslinking of their high affinity IgE receptors (FcεRI) antigen bound immunoglobulin (Ig)E antibody complexes. However, mast can also be mas related G protein-coupled receptor X2 (MRGPRX2), response to a range cationic secretagogues, such as substance P (SP), which is associated with pseudo-allergic reactions. We have previously reported that vitro activation mouse...
Allergic sensitization to aeroallergens develops in response mucosal exposure these allergens. may lead the development of asthma, which is characterized by chronic airway inflammation. The objective this study describe detail a model cockroach allergens absence an exogenous adjuvant.Cockroach extract (CE) was administered mice intranasally (i.n.) daily for 5 days, and days later were challenged with CE 4 consecutive days. A second group received i.n. 3 weeks. Airway hyperresponsiveness...
Mast cells play a distinct role in the innate immune response. Engineered microenvironments for express purpose of influencing mast cell activity will provide novel means designing biomaterials, as well to systematically investigate biology 3D setting. Here, effect nanoscaffolds composed self‐assembling peptides, namely (RADA) 4 , on bone‐marrow‐derived murine (BMMC) is reported. Unlike most studies that stimulate induce adhesion, this results show BMMCs spontaneously adhere artificial...
In this work we analyzed by RT-PCR, the mRNA changes for IL-4, IL-10, TNF and IFN ( induced TSL-1 antigens in a rat mast cell line (HRMC) with mucosal characteristics. The data obtained showed an increase of 65 52 % expression IL-4 respectively decrease 59 55 mRNAs IFNγ IL-10. Our results suggest that induce release from MC regulatory molecules, such as IgE independent mechanism. also provides important information related to ability participate not only effector phase against infectious...
Biodegradable polymeric nanoparticles (NPs) such as poly(lactic-co-glycolic acid) (PLGA) and polyvinyl alcohol (PVA) have been used drug delivery systems for natural synthetic compounds are designed to control the loading release of biodegradable materials target cells, tissues, organs. Eremophilane-type sesquiterpenes anti-inflammatory properties but lipophilic, cytotoxic, not biocompatible with many cells. To determine whether PLGA/PVA could improve biocompatibility sesquiterpenes,...
Dendritic cell (DC) activation induces expression of co-stimulatory surface molecules, as well migration into secondary lymphoid organs, where they activate naïve T-cells. A family plant derivatives, eremophilane-type petasite sesquiterpenes, can regulate the immune system through DC targeting due to their anti-inflammatory effects. Peroxisome proliferator-activated receptor gamma (PPARγ) is involved in inhibition inflammatory responses and induction DCs acquire a mucosal phenotype. Since...
Background A number of common aeroallergens have serine proteinase activity, which is important for allergic sensitization. House dust mite (HDM), and other allergens with activity activate Protease-Activated Receptor-2 (PAR-2). We shown that PAR-2 activation in the airways leads to sensitization concomitantly inhaled antigens, implicating pathogenesis asthma. hypothesized by HDM development Methods extract was administered mice intranasally 5 consecutive days induce One group received a...