A5103260963- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Tea Polyphenols and Effects
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- DNA Repair Mechanisms
- Renal and related cancers
- Microtubule and mitosis dynamics
- Plant Molecular Biology Research
- Mitochondrial Function and Pathology
- Phytochemicals and Antioxidant Activities
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Plant Stress Responses and Tolerance
- Metabolomics and Mass Spectrometry Studies
- Machine Learning in Bioinformatics
- Tissue Engineering and Regenerative Medicine
- Ovarian function and disorders
- Plant tissue culture and regeneration
- Plant Virus Research Studies
Nanjing Agricultural University
2021-2025
Xiamen University
2024
Shenzhen Children's Hospital
2024
Fujian Medical University
2024
Shanghai Jiao Tong University
2014-2023
Xuzhou Medical College
2023
Henan Institute of Science and Technology
2016-2021
Fujian Agriculture and Forestry University
2019-2021
Istituto Tecnico Industriale Alessandro Volta
2021
Weatherford College
2021
Tea plants (Camellia sinensis) are commercially cultivated in >60 countries, and their fresh leaves processed into tea, which is the most widely consumed beverage world. Although several chromosome-level tea plant genomes have been published, they collapsed two haplotypes ignored a large number of allelic variations that may underlie important biological functions this species. Here, we present phased chromosome-scale assembly for an elite oolong cultivar, "Huangdan", well known its high...
Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue quantity (fluence rate) is important for plant life, few studies have focused on the effects different intensity regulation, including tea Here, we performed transcriptomic metabolomic analyses young shoots (one bud two leaves) under three levels supplemental light, low-intensity (LBL, 50 μmol m–2 s–1), medium-intensity (MBL, 100 high-intensity (HBL, 200 s–1). The total number...
The phenylalanine ammonia-lyase (PAL) gene family in tea plants (Camellia sinensis L.) encodes the enzyme that catalyzes first reaction of phenylpropane metabolic pathway. present study aimed to characterize PAL genes plants, and get better insights on CsPALs anthocyanins accumulation. Seven CsPAL were identified characterized by bioinformatics analysis. Systematic analysis was conducted for its phylogenetic relationship, structure, chromosomal location, protein conserved motifs based plant...
It has been demonstrated that through the formation of embryoid bodies (EBs) germ cells can be derived from embryonic stem (ES) cells. Here, we describe a transgene expression approach to derive directly ES in vitro without EB formation. Through ectopic Deleted Azoospermia-Like (Dazl), cell-specific RNA-binding protein, both motile tailed-sperm and oocytes were induced mouse (mES) culture. Furthermore, transient overexpression Dazl led suppression Nanog but cell nuclear antigen mES knockdown...
Garlic has the charisma of a potent remedy and holds its repute therapeutic panacea since dawn civilization. An integrated approach was adopted to evaluate genetic diversity among Chinese garlic cultivars for their antifungal potency as well allicin content distribution and, furthermore; bioassay performed study bio-stimulation mechanism aqueous extracts (AGE) in growth physiology cucumber (Cucumis sativus). Initially, 28 were evaluated against four kinds phytopathogenic fungi; Fusarium...
Abstract Plants have evolved regulatory mechanisms at multiple levels to regulate gene expression in order improve their cold adaptability. However, limited information is available regarding the stress response chromatin and translational levels. Here, we characterize accessibility, transcriptional, landscapes of tea plants vivo under chilling for first time. Chilling significantly affected both transcription translation as well efficiency plants. A total 3010 genes that underwent rapid...
Purple-leaf tea is a phenotype with unique color because of its high anthocyanin content. The special flavor purple-leaf highly different from that green-leaf tea, and main ingredient also economic value. To probe the genetic mechanism phenotypic characteristics leaf color, we conducted widely targeted metabolic transcriptomic profiling. metabolites in flavonoid biosynthetic pathway purple- were compared, results showed phenolic compounds, including acids, flavonoids, tannins, accumulated...
Tea aroma is a key indicator for evaluating tea quality. Although notable success in improvement has been achieved with heterosis breeding technology, the molecular basis underlying remains largely unexplored. Thus, present report studies plant volatile using high-throughput next-generation RNA-seq strategy and gas chromatography–mass spectrometry. Phenotypically, we found higher terpenoid green leaf contents by spectrometry F1 hybrids than their parental lines. Volatile was obvious both...
Pluripotent stem cells derived from neonatal or adult testes are a useful tool to examine the mechanisms of pluripotency and resource for cell-based therapies. However, therapies using these will only benefit males but not females. Recently, female germline (FGSCs) were discovered in ovaries. Whether FGSCs can be converted into pluripotent cells, similar spermatogonial is unknown. Here, we demonstrate that embryonic stem-like (fESLCs) generated within 1 month stably proliferating cultured...
C-repeat binding factors (CBFs) are key signaling genes that can be rapidly induced by cold and bind to the C-repeat/dehydration-responsive motif (CRT/DRE) in promoter region of downstream cold-responsive (COR) genes, which play a vital role plant response low temperature. However, CBF family tea plants has not yet been elucidated, possible target regulated this under temperature still unclear. In study, we identified five CsCBF genome analyzed their phylogenetic tree, conserved domains...
Lipids are one of the most important bioactive compounds, affecting character and quality tea. However, contribution lipids to tea productions is still elusive. Here, we systematically identified lipid profiles green, oolong, black teas in purple-leaf (Jinmingzao, JMZ) green-leaf (Huangdan, HD), respectively.The analysis showed regular accumulation products with different manufacturing processes, among which fatty acids, glycerolipids, glycerophospholipids, sphingolipids contribute...
BackgroundMany studies have demonstrated that microRNAs, a class of small and non-coding RNA molecules, play an important role in the regulation glucose lipid homeostasis. In present study, we sought to investigate function miR-592 development obesity-associated metabolic disorders, including hyperglycemia andinsulin resistance.MethodsThe expression levels were measured liver obese mice humans by quantitative reverse transcription PCR. Loss- gain-of experiments employed explore using locked...
The JASMONATE-ZIM DOMAIN (JAZ) family genes are key repressors in the jasmonic acid signal transduction pathway. Recently, JAZ gene has been systematically characterized many plants. However, this not explored tea plant. In study, 13 CsJAZ were identified plant genome. Phylogenetic analysis showed that proteins from and other plants clustered into 11 sub-groups. transcriptional regulatory network predictive expression pattern analyses suggest these play vital roles abiotic stress responses,...
Albino tea mutants with specific shoot colors (white or yellow) have received increasing attention from researchers due to their unique phenotypes, beneficial metabolites, and special flavor. In this study, novel natural yellow leaf of the same genetic background “Rougui” were obtained, transcriptome metabolite profiles mutant (YR) original green cultivar (GR) investigated. A total 130 significantly changed metabolites (SCMs) 55 differentially expressed genes (DEGs) identified in YR compared...
Abstract Plant resistance inducers represent an alternative strategy that mitigate stress-induced damage in plants. Previously, 2-amino-3-methylhexanoic acid (AMHA), a novel natural plant inducer, was shown to significantly bolster cold tolerance, thermotolerance, and pathogen However, the intricate mechanisms underlying AMHA’s response stress remain elusive. Thus, we investigated physiological transcriptomic analyses of AMHA pretreatment on tea determine its substantial role under stress....
Somatic cell nuclear transfer offers new opportunities for genetic engineering and genome preservation in mammalian animal species. We show that, addition to cumulus cells, cultured adult rabbit fibroblasts are also capable of supporting full-term development after transfer. Nuclear embryos constructed using serum-starved showed a significantly higher developmental rate than non-starved through preimplantation stages. A total 467 were transferred into the oviducts pseudo pregnant mothers....