A5001112094- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Magnetic and Electromagnetic Effects
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Characterization and Applications of Magnetic Nanoparticles
- Electrowetting and Microfluidic Technologies
- Nanoparticle-Based Drug Delivery
- Minerals Flotation and Separation Techniques
- Electrohydrodynamics and Fluid Dynamics
- Geomagnetism and Paleomagnetism Studies
- Modular Robots and Swarm Intelligence
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Material Dynamics and Properties
- Respiratory viral infections research
- nanoparticles nucleation surface interactions
- Microfluidic and Capillary Electrophoresis Applications
Inserm
2017-2023
Centre National de la Recherche Scientifique
2013-2023
Université Grenoble Alpes
2013-2023
Institut pour l'avancée des biosciences
2017-2023
Institut polytechnique de Grenoble
2017-2023
Laboratoire de Génie Électrique de Grenoble
2015-2018
This article presents a magnetically actuated two-way, three-position (+, 0, -), paper-based microfluidic valve that includes neutral position (0)-the first of its kind. The system is highly robust, customizable, and fully automated. advent the ability to precisely control switching frequencies establish new platform for controlled fluid flows in wicking devices. potential utility these valves demonstrated automated, programmed, patterning dyed liquids device akin colorimetric assay but with...
Quantitative nucleic acid detection is widely used in molecular diagnostics for infectious diseases. Here, we demonstrate that the previously developed MLFIA (magnetically localized fluorescent immunoassay) has potential to detect Polymerase Chain Reaction (PCR) and loop-mediated isothermal amplification (LAMP) products using biotinylated primers streptavidin-coated magnetic nanoparticles. The functionalized nanoparticles separate amplified DNA from non-incorporated situ, allowing...
Micro-magnets producing magnetic field gradients as high 106 T m-1 have been used to efficiently trap nanoparticles with a core of just 12 nm in diameter. Particle capture efficiency increases increasing particle concentration. Comparison measured kinetics numerical modelling reveals that threshold concentration exists below which is diffusion-driven and above it convectively-driven. This comparison also shows two-way fluid-particle coupling responsible for the formation convective cells,...
The magnetically localized and wash-free fluorescence immunoassay (MLFIA) is a no-wash assay for directly measuring biomolecule (antigen antibody) concentration, without mixing nor washing steps, from body fluid (serum plasma).
Sexually transmitted infections (STI) remain one of the world's public health priorities: Nearly 400 million people are infected not only in emerging, but also western countries. HIV, HBV and HCV share common infection pathways; thus these 3 diseases recommended to be tested at same time. However, this combined approach is currently mainly available laboratories, seldomly Point-of-care (POC). Consequently, there a need for STI screening POC platform with laboratory-like performance. Such...
We present a fast magnetic immunoassay, combining nanoparticles and micromagnets. High field gradients from micromagnets are used to develop new approach the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed compared with an After optimization, immunoassay exhibits limit detection (40 ng/ml) dynamic range (40-2500 similar that ELISAs developed using same biochemical tools.Micromagnets can be...