A novel expression system for surface display of heterologous proteins on Staphylococcus carnosus cells has been developed. Taking advantage the promoter and secretion signals, including a propeptide region, from lipase gene hyicus cell wall-spanning membrane-binding region protein aureus, efficient an 80-amino-acid peptide malaria blood stage antigen could be achieved. serum albumin binding streptococcal G was used both as general reporter molecule to increase accessibility...

A novel recombinant respiratory syncytial virus (RSV) subunit vaccine, designated BBG2Na, was administered to 108 healthy adults randomly assigned receive 10, 100, or 300 μg of BBG2Na in aluminum phosphate saline placebo. Each subject received 1, 2, 3 intramuscular injections the dose at monthly intervals. Local and systemic reactions were mild, no evidence harmful properties reported. The highest ELISA virus-neutralizing (VN) antibody responses evident 100- 300-μg groups; second third...

Background: Atopic dermatitis (AD) is a common skin disease characterized by recurrent pruritic inflammatory lesions resulting from structural and immune defects of the barrier. Previous studies have shown clinical efficacy Avène thermal spring water in AD, new microorganism, Aquaphilus dolomiae was suspected to contribute these unique properties. The present study evaluated anti-inflammatory, antipruritic, immunomodulatory properties ES0, an original biological extract A. , cell models...

An expression system to allow targeting of heterologous proteins the cell surface Staphylococcus xylosus, a coagulase-negative gram-positive bacterium, is described. The recombinant gene fragments, fused between fragments encoding signal peptide and surface-binding regions staphylococcal protein A, targets resulting fusion outer bacterial via membrane-anchoring region highly charged wall-spanning A. was used secrete containing sequences from malaria blood-stage antigen streptococcal...

What's known on the subject? and What does study add? Pervasive inflammatory infiltrates, mainly composed of chronically activated T cells monocytes/macrophages, have been observed in benign prostatic hyperplasia (BPH). Permixon®, a hexanic lipidosterolic extract Serenoa repens (hexanic LSESr) used to treat urinary dysfunction BPH patients, has anti‐inflammatory activities. This paper provides new insights into properties Permixon®. We report that LSESr inhibits early steps leukocyte...

Site-directed mutagenesis has been employed to engineer the hydrophobic properties of a 101-amino-acid fragment from human respiratory syncytial virus (RSV) major glycoprotein (G protein). When this protein was produced in Escherichia coli, more than 70% gene product found as inclusion bodies, and recovered soluble fraction severely degraded. Substitution two cysteine residues for serine residues, did not significantly change solubility or stability product. In contrast, dramatic increase...

Skin microbiota takes part in the control of cutaneous inflammation. In skin diseases such as atopic dermatitis (AD) dysbiosis and emergence Staphylococcus aureus contribute to pathophysiology disease. New therapeutic approaches consist topical application natural products able counteract S. effects through activation resident immune cells producing anti-inflammatory cytokines IL-10.This study investigates potential immunosuppressive properties Aquaphilus dolomiae (Neisseriaceae), a...

Abstract Titanium dioxide (TiO 2 ) nanoparticles were successfully synthesized using titanium isopropoxide as a substrate and green tea extract reducing agent in the synthesis process. The structural characteristics of TiO analyzed advanced techniques such scanning electron microscope, energy dispersive X-ray, X-ray diffraction analysis, N adsorption/desorption isotherm. with crystal size 21.04 nm calculated Debye–Scherer equation, indicating dominant anatase structure. nanomaterial...

Alum-adsorbed BBG2Na, a recombinant vaccine derived in part from the respiratory syncytial virus (RSV) subgroup A G protein, induced moderate antibody titers after 1 immunization 1-week-old mice but conferred complete lung protection upon RSV challenge. The anti-BBG2Na IgG1-IgG2a neonatal isotype profile was suggestive of dominant Th2 responses compared with those adults. Formulation BBG2Na Th1-driving adjuvant efficiently shifted toward more balanced and adultlike without compromising its...

Maternal and neonatal immunization were evaluated for their capacity to induce protective immunity against respiratory syncytial virus (RSV) lower tract infections in early life. Murine models studied by use of a novel recombinant vaccine candidate, designated BBG2Na, which was derived part from the RSV (Long) G protein. resulted passive transfer high levels RSV-A antibodies offspring, protected them challenge up 14 weeks. Indeed, protection correlated with detection serum. Neonatal BBG2Na...

ABSTRACT A recombinant fusion protein (BBG2Na) comprising the central conserved domain of respiratory syncytial virus subgroup (RSV-A) (Long) G (residues 130 to 230) and an albumin binding streptococcal was shown previously protect mouse upper (URT) lower (LRT) tracts against intranasal RSV challenge (U. F. Power, H. Plotnicky-Gilquin, T. Huss, A. Robert, M. Trudel, S. Stahl, Uhlén, N. Nguyen, Binz, Virology 230:155–166, 1997). Panels monoclonal antibodies (MAbs) synthetic peptides were...

The heterologous surface expression of the cholera toxin B subunit (CTB) from Vibro cholerae in two staphylococcal species, Staphylococcus xylosus and carnosus, has been investigated. gene encoding native CTB (103 amino acids) was introduced into constructs chimeric receptors designed to be translocated anchored on outer cell staphylococci. Since functionality is correlated with its ability form pentamers capacity pentameric bind GM1 ganglioside, both accessibility surface-displayed were...

The polypeptide of aa 130-230 the G protein (G2Na) respiratory syncytial virus (RSV) was fused to BB, albumin-binding region streptococcal protein, producing BBG2Na, which induced protective immune responses in rodent models. Evaluation response mice immunized with BBG2Na adjuvant alhydrogel revealed high amounts interleukin (IL)-5 and some IL-4 splenocytes restimulated vitro. This is compatible a Th2 response. activation pathway such further supported by detection IL-5 G2Na-specific IgE...

Respiratory syncytial virus (RSV) is an important cause of severe upper and lower respiratory disease in infants the elderly. There are 2 main RSV subtypes A B. recombinant vaccine was designed based on central domain RSV-A attachment G protein which we had previously named G2Na (aa130-230). Here evaluated immunogenicity, persistence antibody (Ab) response protective efficacy induced rodents by: (i) fused to DT (Diphtheria toxin) fragments cotton rats. fusion did not potentiate neutralizing...