A5042537374- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Viral gastroenteritis research and epidemiology
- Tracheal and airway disorders
- Virus-based gene therapy research
- Pediatric health and respiratory diseases
- Pneumonia and Respiratory Infections
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- interferon and immune responses
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Asthma and respiratory diseases
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Congenital Diaphragmatic Hernia Studies
- vaccines and immunoinformatics approaches
- Spectroscopy Techniques in Biomedical and Chemical Research
- Marine Bivalve and Aquaculture Studies
- Parvovirus B19 Infection Studies
- RNA and protein synthesis mechanisms
- COVID-19 Clinical Research Studies
Queen's University Belfast
2015-2024
Open Geospatial Consortium
2019
Queens University
2007-2015
Institute of Infection and Immunity
1985-2015
Center of Immunology Pierre Fabre
1995-2007
University of Geneva
1999
World Health Organization
1999
St. Jude Children's Research Hospital
1992-1994
University of Tennessee Health Science Center
1994
University College Cork
1989-1990
ABSTRACT Respiratory syncytial virus (RSV) is an important causative agent of lower respiratory tract infections in infants and elderly individuals. Its fusion (F) protein critical for infection. It targeted by several investigational antivirals palivizumab, a humanized monoclonal antibody used prophylactically considered at high risk severe RSV disease. ALX-0171 trimeric Nanobody that binds the antigenic site II F with subnanomolar affinity. demonstrated vitro neutralization superior to...
The efficacious delivery of antigens to antigen-presenting cells (APCs), in particular, dendritic (DCs), and their subsequent activation remains a significant challenge the development effective vaccines. This study highlights potential dissolving microneedle (MN) arrays laden with nanoencapsulated antigen increase vaccine immunogenicity by targeting specifically contiguous DC networks within skin. Following situ uptake, skin-resident DCs were able deliver antigen-encapsulated...
Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial extracellular environments and here we show that respiratory syncytial virus (RSV) herpes simplex type 1 (HSV-1) rich distinctive different fluids. Moreover, pre-coating differentially affects viral infectivity immune cell activation. In addition, demonstrate...
Respiratory syncytial virus (RSV) is the major viral cause of severe pulmonary disease in young infants worldwide. However, mechanisms by which RSV causes humans remain poorly understood. To help bridge this gap, we developed an ex vivo/in vitro model infection based on well-differentiated primary pediatric bronchial epithelial cells (WD-PBECs), targets vivo. Our RSV/WD-PBEC demonstrated remarkable similarities to hallmarks infant lungs. These included restriction noncontiguous or small...
ABSTRACT Respiratory syncytial virus (RSV) infection causes bronchiolitis and pneumonia in infants. RSV has a linear single-stranded RNA genome encoding 11 proteins, 2 of which are nonstructural (NS1 NS2). specifically downregulates STAT2 protein expression, thus enabling the to evade host type I interferon response. Degradation requires proteasomal activity is dependent on expression NS1 NS2 (NS1/2). Here we investigate whether NS proteins can assemble ubiquitin ligase (E3) enzymes target...
Receptors implicated in cough hypersensitivity are transient receptor potential vanilloid 1 (TRPV1), cation channel, Subfamily A, Member (TRPA1) and acid sensing ion channel 3 (ASIC3). Respiratory viruses, such as respiratory syncytial virus (RSV) measles (MV) may interact directly and/or indirectly with these receptors on sensory nerves epithelial cells the airways. We used vitro models of neurones (SHSY5Y or differentiated IMR-32 cells) human bronchial epithelium (BEAS-2B well primary...
Rationale: Respiratory syncytial virus (RSV) is a major pathogen that primarily infects airway epithelium. Most infants suffer mild upper respiratory tract (URT) symptoms, whereas approximately one-third progress to lower (LRT) involvement. Despite the ubiquity of URT infection, little known about relative cytopathogenesis RSV infection in infant and LRT.Objectives: This study aimed compare nasal- bronchial-derived epithelium from same individuals using novel models derived...
Respiratory syncytial virus (RSV) is the major cause of viral lower respiratory tract illness in children. In contrast to RSV prototypic strain A2, clinical isolate 2-20 induces airway mucin expression mice, a clinically relevant phenotype dependent on fusion (F) protein strain. Epidermal growth factor receptor (EGFR) plays role other systems; therefore, we hypothesized that F stimulates EGFR signaling. Infection cells with chimeric strains A2-2-20F and A2-2-20GF or over-expression resulted...
The emergence of SARS-CoV-2 variants has exacerbated the COVID-19 global health crisis. Thus far, all carry mutations in spike glycoprotein, which is a critical determinant viral transmission being responsible for attachment, receptor engagement and membrane fusion, an important target immunity. Variants frequently bear truncations flexible loops N-terminal domain (NTD) spike; functional importance these modifications remained poorly characterised. We demonstrate that NTD deletions are...
A novel recombinant respiratory syncytial virus (RSV) subunit vaccine, designated BBG2Na, was administered to 108 healthy adults randomly assigned receive 10, 100, or 300 μg of BBG2Na in aluminum phosphate saline placebo. Each subject received 1, 2, 3 intramuscular injections the dose at monthly intervals. Local and systemic reactions were mild, no evidence harmful properties reported. The highest ELISA virus-neutralizing (VN) antibody responses evident 100- 300-μg groups; second third...
Abstract Background Human respiratory syncytial virus (RSV) causes severe disease in infants. Airway epithelial cells are the principle targets of RSV infection. However, mechanisms by which it poorly understood. Most pathogenesis data derived using laboratory-adapted prototypic strains. We hypothesized that such strains may be representative recent clinical isolates terms virus/host interactions primary human bronchial (PBECs). Methods To address this hypothesis, we isolated three from...
ABSTRACT Airway epithelium is the primary target of many respiratory viruses. However, virus induction and antagonism host responses by human airway remains poorly understood. To address this, we developed a model syncytial (RSV) infection based on well-differentiated pediatric bronchial epithelial cell cultures (WD-PBECs) that mimics hallmarks RSV disease in infants. most important viral pathogen young infants worldwide. We found induces potent antiviral state WD-PBECs was mediated part...
The elimination of sewage effluent-associated poliovirus, Escherichia coli, and a 22-nm icosahedral coliphage by the common mussel, Mytilus edulis, was studied. Both laboratory-and commercial-scale recirculating, UV depuration systems were used in this study. In laboratory system, logarithms E. levels reduced 1.86, 2.9, 2.16, respectively, within 52 h depuration. relative patterns rates three organisms suggest that they are eliminated from mussels different mechanisms during under suitable...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of disease-19 (COVID-19) pandemic, was identified in late 2019 and caused >5 million deaths by February 2022. To date, targeted antiviral interventions against COVID-19 are limited. The spectrum SARS-CoV-2 infection ranges from asymptomatic to fatal disease. However, reasons for varying outcomes yet be elucidated. Here we show that an endogenously activated interferon lambda (IFNλ1) pathway leads resistance infection....
Site-directed mutagenesis has been employed to engineer the hydrophobic properties of a 101-amino-acid fragment from human respiratory syncytial virus (RSV) major glycoprotein (G protein). When this protein was produced in Escherichia coli, more than 70% gene product found as inclusion bodies, and recovered soluble fraction severely degraded. Substitution two cysteine residues for serine residues, did not significantly change solubility or stability product. In contrast, dramatic increase...