A5069325199- RNA Research and Splicing
- Nuclear Structure and Function
- Lipid Membrane Structure and Behavior
- Advancements in Transdermal Drug Delivery
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Organic Electronics and Photovoltaics
- Viral gastroenteritis research and epidemiology
- Cell Image Analysis Techniques
- Respiratory viral infections research
- DNA Repair Mechanisms
- Force Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Skin and Cellular Biology Research
- Conducting polymers and applications
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Microtubule and mitosis dynamics
- Autoimmune Bullous Skin Diseases
- Surfactants and Colloidal Systems
- Genomics and Chromatin Dynamics
- Sphingolipid Metabolism and Signaling
- Advancements in Battery Materials
Karolinska Institutet
2010-2022
Max Planck Institute of Molecular Physiology
2020
Karolinska University Hospital
2007-2015
Linköping University
2009
The skin barrier is fundamental to terrestrial life and its evolution; it upholds homeostasis protects against the environment. Skin capacity controlled by lipids that fill extracellular space of skin's surface layer--the stratum corneum. Here we report on determination molecular organization lipid matrix in situ, near-native state, using a methodological approach combining very high magnification cryo-electron microscopy (EM) vitreous section defocus series, modeling, EM simulation. are...
Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial extracellular environments and here we show that respiratory syncytial virus (RSV) herpes simplex type 1 (HSV-1) rich distinctive different fluids. Moreover, pre-coating differentially affects viral infectivity immune cell activation. In addition, demonstrate...
Electron tomography has been used for analyzing the active layer in a polymer solar cell, bulk heterojunction of an alternating copolymer fluorene and derivative fullerene. The method supplies three-dimensional representation morphology film, where domains with different scattering properties may be distinguished. reconstruction shows good contrast between two phases included film demonstrates that electron is adequate tool investigations nanostructure amorphous materials cells.
We present a new approach to simulate electron cryo-microscope images of biological specimens. The framework for simulation consists two parts; the first is phantom generator that generates model specimen suitable simulation, second transmission microscope simulator. calculates scattering potential an atomic structure in aqueous buffer and allows user define distribution molecules simulated image. simulator includes well defined electron–specimen interaction based on scalar Schrödinger...
The synaptonemal complex transiently stabilizes pairing interactions between homologous chromosomes during meiosis. Assembly of the is mediated through integration opposing transverse filaments into a central element, process that poorly understood. We have, here, analyzed localization filament protein SYCP1 and element proteins SYCE1, SYCE2 SYCE3 within region in mouse spermatocytes using immunoelectron microscopy. Distribution immuno-gold particles lateral view complex, supported by...
Control of the nanoscale morphology donor−acceptor material blends in organic solar cells is critical for optimizing photovoltaic performances. The influence intrinsic (acceptor materials) and extrinsic (donor:acceptor weight ratio, substrate, solvent) parameters was investigated, by atomic force microscopy (AFM) electron tomography (ET), on phase separation a low-band-gap alternating polyfluorene copolymers (APFO-Green9) with [6,6]-phenyl-C71-butyric acid methyl ester ([70]PCBM)....
In organized tissues, the precise geometry and overall shape are critical for specialized functions that cells carry out. Odontoblasts major matrix-producing of tooth have also been suggested to participate in sensory transmission. However, refined morphologic data on these important limited, which hampers analysis understanding their cellular functions. We took advantage fluorescent color-coding genetic tracing visualize reconstruct 3 dimensions single odontoblasts, pulp cells, assemblages....
In vertebrates, skin upholds homeostasis by preventing body water loss. The skin's permeability barrier is located intercellularly in the stratum corneum and consists of stacked lipid lamellae composed ceramides, cholesterol, free fatty acids. We have combined cryo-electron microscopy with molecular dynamics modeling electron simulation our analysis lamellae's formation, a maturation process beginning granulosum ending corneum. Previously, we revealed initial- end-stage organizations. this...
A specific messenger ribonucleoprotein (RNP) particle, Balbiani ring (BR) granules in the dipteran Chironomus tentans, can be visualized during passage through nuclear pore complex (NPC). We have now examined transport basket preceding actual translocation NPC. The consists of eight fibrils anchored to NPC core by nucleoprotein Nup153. On injection anti-Nup153, BR is blocked. Many are retained on top basket, whereas no seen transit Interestingly, effect Nup153 seems distant from...
Improved knowledge of the topology lamellar bodies is a prerequisite for molecular-level understanding skin barrier formation, which in turn may provide clues as to underlying causes barrier-deficient disease. The aim this study was examine key question continuity vs. discreteness body system using 3 highly specialized and complementary 3-dimensional (3D) electron microscopy methodologies; tomography vitreous sections (TOVIS), freeze-substitution serial section (FS-SET), focused ion beam...
The skin's permeability barrier consists of stacked lipid sheets splayed ceramides, cholesterol and free fatty acids, positioned intercellularly in the stratum corneum. We report here on early stage skin formation taking place inside tubuloreticular system secretory cells topmost viable epidermis intercellular space between process was analysed situ its near-native state, using cryo-EM combined with molecular dynamics modeling EM simulation. Stacks lamellae appear towards periphery they are...
In a synaptic active zone, vesicles aggregate around densely staining structure called the presynaptic density. We focus on its three-dimensional architecture and major molecular component in locust. used electron tomography to study density synapses made brain by identified second-order neuron of ocelli. Here, close zone are organized two rows either side density, level organization not previously reported insect central synapses. The row that is closest density's base includes docked with...
Organic electronic materials and nanostructures have been studied with the use of electron tomography. Nanostructured including contrast enhancing features double tilt data collection has employed to improve reconstructions. Tomography reconstructions active layers organic solar cells, where various preparation techniques used, analysed compared device behaviour. Small changes in procedures may lead large differences morphology performance, results also indicate a complex relation between these.
Abstract Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial extracellular environments and here we show that respiratory syncytial virus (RSV) herpes simplex 1 (HSV-1) rich distinctive different fluids. Moreover, pre-coating differentially affects viral infectivity immune cell activation. Additionally,...
Y‐box proteins constitute an evolutionarily conserved family of DNA‐ and RNA‐binding involved in the regulation transcription translation. In dipteran Chironomus tentans , a homologue to vertebrate protein YB‐1 was recently characterized designated ctYB‐1. It is transferred from nucleus cytoplasm bound mRNA likely affect appears two size variants, p40 p50. We further analysed variants their interaction with mRNA. Southern blot analysis, situ hybridization RT‐PCR analysis suggested that there...
A green, though black, sustainable and low‐cost carbon material—charcoal produced from wood—is developed for electricity storage. Charcoal electrodes are fabricated by ball‐milling charcoal adding protein nanofibril binders. The electrode presents a capacitance of 360 F g −1 conductivity 0.2 S m . pair redox peaks is observed in the cyclic voltammetry assigned to originate quinone groups. Compared with other wooden electrodes, these display better cycling stability 88% capacity retention...
Visual understanding is a key aspect when studying electron tomography data-sets, aside quantitative assessments such as registration of high-resolution structures. We here present the free software tool ProViz (Protein Visualization) for visualisation and template matching in tomograms biological samples. The contains methods tools which we have developed, adapted computationally optimised easy intuitive analysis with low signal-to-noise ratio. complements existing application field serves...