Protein–RNA interactions are fundamentally important in understanding cellular processes. In particular, non-coding RNA–protein play an role to facilitate biological functions signalling, transcriptional regulation, and even the progression of complex diseases. However, experimental determination protein–RNA remains time-consuming labour-intensive. Here, we develop a novel extended naïve-Bayes-classifier for de novo prediction interactions, only using protein RNA sequence information....

Percutaneous endoscopic lumbar discectomy (PELD) is an increasingly applied minimally invasive procedure that has several advantages in the treatment of disc herniation (LDH). However, recurrent LDH (rLDH) become a concerning postoperative complication. It remains difficult to establish consensus and draw reliable conclusions regarding risk factors for rLDH.This retrospective study aimed investigate associated with rLDH at L4-5 level after percutaneous transforaminal (PETD).A total 654...

In spinal fusion surgery, total blood loss (TBL) is composed of visible from the surgical field and wound drainage, hidden (HBL). Until now, no published studies exist reporting effect topical use tranexamic acid (tTXA) on HBL in patients undergoing posterior lumbar surgery. This study aimed to explore tTXA during primary Between September 2014 2016, 100 adult (age > 18 years) with disc herniation or stenosis instrumented fusions at 1 institution were divided into control groups. The outcome...

Abstract Background: Posterior lumbar interbody fusion (PLIF) surgery is associated with significant blood loss; however, few studies have focused on hidden loss (HBL) in PLIF or its regulatory factors. The purpose of this study was to explore the HBL as well influence tranexamic acid (TXA) PLIF. Methods: We performed a randomized controlled trial (RCT) and recruited patients undergoing into from November 2013 April 2017. All participants were assigned one 2 groups according simple equal...

This is a randomized controlled trial research to assess the hemostatic efficacy of gelatin sponge, collagen and topical use tranexamic acid (TXA) on postoperative blood loss in posterior spinal fusion surgeries. We recruited patients with degenerative diseases into study from November 2013 October 2016. All participants were assigned 3 groups using simple, equal-probability randomization scheme: group A control utilizing while B C are experimental groups, applying sponge TXA respectively....

Study Design. A retrospective case-control study. Objective. To compare postoperative blood loss, amount of allogeneic transfusion, removal time drainage tube, length hospital stay, and complications associated with tranexamic acid (TXA). Summary Background Data. Spinal fusion surgery can be significant loss. the best our knowledge, very few published studies exist reporting effect topical use (tTXA) on decreasing loss in patients undergoing posterior lumbar spinal fusions. Methods. We...

Metagenomic next-generation sequencing (mNGS) is a powerful yet unbiased method to identify pathogens in suspected infections. However, little known about its clinical effectiveness. The present study aimed assess the efficacy of mNGS routine practice.In this single-center retrospective cohort study, 518 patients with infectious diseases were assessed for inclusion. Among them, each patient had undergone testing; 407 both microbial culture and testing. result testing was compared performed...

To investigate the preoperative radiological risk factors for recurrent lumbar disc herniation (rLDH) within 1 year after percutaneous endoscopic transforaminal discectomy (PETD).A retrospective case-control study was conducted. Between January 2013 and November 2019, a total of 1210 patients with single-level L4/5 LDH who underwent PETD were enrolled in present study. In total, 62 rLDH diagnosed collected based on clinical manifestations, 224 non-rLDH controls selected from remaining 1148...

Intervertebral disc degeneration (IDD) is a chronic degenerative and age-dependent process characterized by aberrant apoptosis, proliferation, synthesis, catabolism of the extracellular matrix nucleus pulposus (NP) cells. Recently, studies showed that circular RNAs play important roles in development many diseases. However, role circRNAs IDD remains unknown. We circ_0134111 level was overexpressed tissue samples as compar-ed to control tissues. The upregulation more drastic moderate severe...

Optimal surgical technique to treat lumbar disc herniation (LDH) remains controversial. We described a small incision discectomy (SID), and evaluate its safety efficacy. A retrospective study involving 98 consecutive patients with LDH managed by SID was conducted. All were followed up for 5 years. Outcomes included visual analogue scale (VAS), Japanese Orthopedic Association (JOA), operative time, length of incision, blood loss, hospital stay, hospitalization costs, x-ray exposure,...

Growing evidence indicates that Long noncoding RNAs contribute to cell differentiation, invasion, metabolism, proliferation and metastasis. However, the potential role of LINC01121 in progression intervertebral disc degeneration (IDD) remains unclear.LINC01121, matrix metalloprotease (MMP)-16 miR-150-5p expression was determined by a quantitative-reverse transcriptase-polymerase chain reaction assay. Inflammatory cytokines level measured an enzyme-linked immunosorbent assay counting kit-8...

Abstract Background Fungal spondylodiscitis is a rare infectious disease. The secondary lumbar spinal stenosis and postoperative discal pseudocyst were even rarer. surgical interventions disputed, yet endoscopic robot-assisted techniques may be helpful under different circumstances. Case presentation A 62-year-old female was diagnosed as at the L4/5 level posterolateral debridement performed after invalid conservative therapy. Causative organism culture revealed fungus, Candida tropicalis ....

Spinal fusion surgery is associated with significant blood loss, which may result in potential clinical complications, it necessary to take safe and effective measures reduce loss surgery. We perform this study assess the impact of three different hemostatic materials on perioperative loss. performed a Randomized Controlled Trial research recruited patients lumbar disease into between November 2013 March 2015. All participants were randomly assigned 3 groups using simple equal probability...