- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Horticultural and Viticultural Research
- Plant and Fungal Interactions Research
- Plant Disease Management Techniques
- Plant Pathogens and Fungal Diseases
- Nuts composition and effects
- Air Quality and Health Impacts
- Postharvest Quality and Shelf Life Management
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Atmospheric chemistry and aerosols
- Air Quality Monitoring and Forecasting
- Fungal Plant Pathogen Control
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Phytoplasmas and Hemiptera pathogens
- Vehicle emissions and performance
- Genomics and Phylogenetic Studies
- Plant pathogens and resistance mechanisms
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Photochemistry and Electron Transfer Studies
Centro de Edafología y Biología Aplicada del Segura
2015-2024
Consejo Superior de Investigaciones Científicas
2010-2023
Universidad de Santiago de Chile
1999-2023
Centre d'Etudes Biologiques de Chizé
2006-2015
Universidad Central "Marta Abreu" de las Villas (UCLV)
2013
Unidades Centrales Científico-Técnicas
2011
Institut National de la Recherche Agronomique
2009
University of O'Higgins
2009
Universidad Bernardo O'Higgins
2009
Genetics and Improvement of Fruit and Vegetables
2005-2008
Abstract. The oxidation capacity of the highly polluted urban area Santiago, Chile has been evaluated during a summer measurement campaign carried out from 8–20 March 2005. hydroxyl (OH) radical budget was employing simple quasi-photostationary-state model (PSS) constrained with simultaneous measurements HONO, HCHO, O3, NO, NO2, j(O1D), j(NO2), 13 alkenes and meteorological parameters. In addition, zero dimensional photochemical box based on Master Chemical Mechanism (MCMv3.1) used to...
In this work, a recombinant plum pox virus (PPV, Sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and expression at the subcellular level in pea plants (cv. Alaska). PPV had produced chlorotic spots as well necrotic oldest leaves 13–15 d post-inoculation. At 15 post-inoculation, was present areas, shown by fluorescence signal presence of protein. same an accumulation reactive oxygen species noticed. Studies with laser confocal electron microscopy...
This work describes, for the first time, changes taking place in antioxidative system of leaf apoplast response to plum pox virus (PPV) different Prunus species showing susceptibilities PPV. The presence p-hydroxymercuribenzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (class I APX) and pHMB-insensitive APX III APX), superoxide dismutase (SOD), (POX), NADH-POX, polyphenoloxidase (PPO) was described from both peach apricot leaves. PPV infection produced antioxidant species, depending...
Generating chromosome-level, haplotype-resolved assemblies of heterozygous genomes remains challenging. To address this, we developed gamete binning, a method based on single-cell sequencing haploid gametes enabling separation the whole-genome reads into haplotype-specific sets. After assembling each haplotype, contigs are scaffolded to chromosome level using genetic map derived from gametes. We assemble two diploid apricot tree 445 individual pollen grains. The haplotype (N50: 25.5 and 25.8...
In this study, the effect of long‐term plum pox virus (PPV) infection on response certain antioxidant enzymes at subcellular level was studied in peach plants ( Prunus persica (L.) Batch) (cv. GF305), which are characterized by great susceptibility to virus. infected plants, a decrease efficiency excitation energy capture PSII F v ′/ m ′) observed, accompanied non‐photochemical quenching (NPQ). p ‐Hydroxy‐mercury benzoic acid (pHMB)‐insensitive ascorbate peroxidase (APX) activity (class III...
Differences in gene expression were studied after Plum pox virus (PPV, sharka disease) infection peach GF305 leaves with and without symptoms using RNA-Seq. For each sample, more than 80% of 100-nucleotide paired-end (PE) Illumina reads aligned on the reference genome. In symptomatic a significant proportion mapped to PPV genomes (1.04% compared 0.00002% non-symptomatic leaves), allowing for ultra-deep assembly complete genome isolate used (9775 nucleotides, missing only 11 nucleotides at 5'...
Abstract The stone fruit genus Prunus , within the family Rosaceae comprises more than 230 species, some of which have great importance or value as ornamental crops. are affected by numerous viruses and viroids linked to vegetative propagation practices in many cultivated species. To date, 44 three been described 9 main Seven these one viroid identified hosts last 5 years. This work addresses recent advances prospects study affecting mostly concerning detection characterisation agents...
The effect of plum pox virus (PPV) infection on the response some antioxidant enzymes was studied in two apricot cultivars, which behaved differently against PPV infection: cultivar Real Fino (susceptible) and Stark Early Orange (cv. SEO, resistant). In susceptible cultivar, produced a decrease Φ PSII , F ′ v / m Q p . drop ‐hydroxy mercury benzoic acid (pHMB)‐sensitive ascorbate peroxidase, dehydroascorbate reductase peroxidase soluble fraction from plants, whereas resistant...
Sharka, a disease caused by plum pox virus (PPV), has significant economic impact on fruit tree production. In this work, we analysed the effect of (2,1,3)-benzothiadiazole (BTH) and L-2-oxo-4-thiazolidine-carboxylic acid (OTC) plant growth content. OTC reduced sharka symptom, stimulated alleviated PPV-induced oxidative stress, indicated lack changes in some stress parameters. PPV infection chloroplast electron transport efficiency. However, presence BTH or OTC, no chlorophyll fluorescence...
Throughout history, new strategies and technologies have played a key role in promoting the development of agriculture. New led to substantial improvements crop productivity fruit quality. The cultivation peach apricot controlled greenhouse conditions is one such strategy. aim protected these species greenhouses grow trees by modifying natural environment tree, including harvest duration, which can be extended. This should improve yield, thus increasing benefit–cost ratio, especially case...
High-throughput phenotyping is addressing the current bottleneck in within breeding programs. Imaging tools are becoming primary resource for improving efficiency of processes and providing large datasets genomic selection approaches. The advent AI brings new advantages by enhancing methods using imaging, making them more accessible to In this context, we have developed an open Python workflow analyzing morphology heritable morphometric traits AI, which can be applied fruits other plant...